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Genome response to tissue plasminogen activator in experimental ischemic stroke.

Jickling GC, Zhan X, Ander BP, Turner RJ, Stamova B, Xu H, Tian Y, Liu D, Davis RR, Lapchak PA, Sharp FR - BMC Genomics (2010)

Bottom Line: Tissue plasminogen activator (tPA) is known to have functions beyond fibrinolysis in acute ischemic stroke, such as blood brain barrier disruption.Genes identified had functions related to modulation of immune cells. tPA gene expression was found to be dependent on the reperfusion status of cerebral vasculature.These non-fibrinolytic activities of tPA in the blood serve to better understand tPA-related complications.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Neurology and M,I,N,D, Institute, University of California at Davis, Sacramento, California 95817, USA. gcjickling@ucdavis.edu

ABSTRACT

Background: Tissue plasminogen activator (tPA) is known to have functions beyond fibrinolysis in acute ischemic stroke, such as blood brain barrier disruption. To further delineate tPA functions in the blood, we examined the gene expression profiles induced by tPA in a rat model of ischemic stroke.

Results: tPA differentially expressed 929 genes in the blood of rats (p or= /1.2/). Genes identified had functions related to modulation of immune cells. tPA gene expression was found to be dependent on the reperfusion status of cerebral vasculature. The majority of genes regulated by tPA were different from genes regulated by ischemic stroke.

Conclusions: tPA modulates gene expression in the blood of rats involving immune cells in a manner that is dependent on the status of vascular reperfusion. These non-fibrinolytic activities of tPA in the blood serve to better understand tPA-related complications.

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Venn diagram of numbers of gene probes differentially regulated by tPA, ischemic stroke, and tPA-ischemic stroke interaction from the ANOVA analysis (p ≤ 0.05, fold change ≥ /1.2/).
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Figure 1: Venn diagram of numbers of gene probes differentially regulated by tPA, ischemic stroke, and tPA-ischemic stroke interaction from the ANOVA analysis (p ≤ 0.05, fold change ≥ /1.2/).

Mentions: An interaction was identified between tPA and ischemic stroke. There were 2332 gene probes regulated by ischemic stroke and 983 gene probes regulated by the interaction of ischemic stroke with tPA (p ≤ 0.05, fold change ≥ /1.2/) (Figure 1). There were 130 genes regulated by ischemic stroke and tPA, 527 genes regulated by tPA and not by ischemia, and 1944 genes regulated by ischemic stroke that were not regulated by tPA (p ≤ 0.05, fold change ≥ /1.2/).


Genome response to tissue plasminogen activator in experimental ischemic stroke.

Jickling GC, Zhan X, Ander BP, Turner RJ, Stamova B, Xu H, Tian Y, Liu D, Davis RR, Lapchak PA, Sharp FR - BMC Genomics (2010)

Venn diagram of numbers of gene probes differentially regulated by tPA, ischemic stroke, and tPA-ischemic stroke interaction from the ANOVA analysis (p ≤ 0.05, fold change ≥ /1.2/).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2875237&req=5

Figure 1: Venn diagram of numbers of gene probes differentially regulated by tPA, ischemic stroke, and tPA-ischemic stroke interaction from the ANOVA analysis (p ≤ 0.05, fold change ≥ /1.2/).
Mentions: An interaction was identified between tPA and ischemic stroke. There were 2332 gene probes regulated by ischemic stroke and 983 gene probes regulated by the interaction of ischemic stroke with tPA (p ≤ 0.05, fold change ≥ /1.2/) (Figure 1). There were 130 genes regulated by ischemic stroke and tPA, 527 genes regulated by tPA and not by ischemia, and 1944 genes regulated by ischemic stroke that were not regulated by tPA (p ≤ 0.05, fold change ≥ /1.2/).

Bottom Line: Tissue plasminogen activator (tPA) is known to have functions beyond fibrinolysis in acute ischemic stroke, such as blood brain barrier disruption.Genes identified had functions related to modulation of immune cells. tPA gene expression was found to be dependent on the reperfusion status of cerebral vasculature.These non-fibrinolytic activities of tPA in the blood serve to better understand tPA-related complications.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Neurology and M,I,N,D, Institute, University of California at Davis, Sacramento, California 95817, USA. gcjickling@ucdavis.edu

ABSTRACT

Background: Tissue plasminogen activator (tPA) is known to have functions beyond fibrinolysis in acute ischemic stroke, such as blood brain barrier disruption. To further delineate tPA functions in the blood, we examined the gene expression profiles induced by tPA in a rat model of ischemic stroke.

Results: tPA differentially expressed 929 genes in the blood of rats (p or= /1.2/). Genes identified had functions related to modulation of immune cells. tPA gene expression was found to be dependent on the reperfusion status of cerebral vasculature. The majority of genes regulated by tPA were different from genes regulated by ischemic stroke.

Conclusions: tPA modulates gene expression in the blood of rats involving immune cells in a manner that is dependent on the status of vascular reperfusion. These non-fibrinolytic activities of tPA in the blood serve to better understand tPA-related complications.

Show MeSH
Related in: MedlinePlus