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Prenatal allergen and diesel exhaust exposure and their effects on allergy in adult offspring mice.

Corson L, Zhu H, Quan C, Grunig G, Ballaney M, Jin X, Perera FP, Factor PH, Chen LC, Miller RL - Allergy Asthma Clin Immunol (2010)

Bottom Line: However, the effects of prenatal environmental exposures on adult offspring have not been well-studied.At age 9-10 weeks, their offspring were sensitized and challenged with A. fumigatus.These results suggest that, in this model, allergen and/or diesel administration during pregnancy may be associated with protection from developing systemic and airway allergic immune responses in the adult offspring.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA. rlm14@columbia.edu

ABSTRACT

Background: Multiple studies have suggested that prenatal exposure to either allergens or air pollution may increase the risk for the development of allergic immune responses in young offspring. However, the effects of prenatal environmental exposures on adult offspring have not been well-studied. We hypothesized that combined prenatal exposure to Aspergillus fumigatus (A. fumigatus) allergen and diesel exhaust particles will be associated with altered IgE production, airway inflammation, airway hyperreactivity (AHR), and airway remodeling of adult offspring.

Methods: Following sensitization via the airway route to A. fumigatus and mating, pregnant BALB/c mice were exposed to additional A. fumigatus and/or diesel exhaust particles. At age 9-10 weeks, their offspring were sensitized and challenged with A. fumigatus.

Results: We found that adult offspring from mice that were exposed to A. fumigatus or diesel exhaust particles during pregnancy experienced decreases in IgE production. Adult offspring of mice that were exposed to both A. fumigatus and diesel exhaust particles during pregnancy experienced decreases in airway eosinophilia.

Conclusion: These results suggest that, in this model, allergen and/or diesel administration during pregnancy may be associated with protection from developing systemic and airway allergic immune responses in the adult offspring.

No MeSH data available.


Related in: MedlinePlus

Perivascular, peribronchial airway inflammation and airway remodeling in offspring. a. Perivascular, peribronchial airway inflammation in offspring of A. fumigatus. Composite scores were obtained following 5 or 6 doses of A. fumigatus. Significant differences across groups were not detected. p = 0.11 on ANOVA. Perivascular and peribronchial inflammation were scored as follows [13]: 1 = normal with very few inflammatory cells bordering the arteries or airways; 2 = scattered inflammatory cells surrounding the artery or airway up to two rings in depth; 3 = cell cuffs or clusters of inflammatory cells surrounding the artery or airway three rings or more in depth. b. Arterial remodeling in offspring. Offspring from mothers treated during pregnancy as outlined in Fig. 1 were exposed to A. fumigatus intranasally with 5-6 doses starting at 9-10 weeks of age. Arterial remodeling was scored as described [13] and in Figure 3b. Significant differences across groups were not detected. p = 0.183 on ANOVA. Arterial remodeling was scored as follows [13]: 1 = normal; 2 = thickened vascular wall with intact lumen and circular media; 3 = obstructed lumen and thickened wall lined with disorganized layers of cells.
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Figure 6: Perivascular, peribronchial airway inflammation and airway remodeling in offspring. a. Perivascular, peribronchial airway inflammation in offspring of A. fumigatus. Composite scores were obtained following 5 or 6 doses of A. fumigatus. Significant differences across groups were not detected. p = 0.11 on ANOVA. Perivascular and peribronchial inflammation were scored as follows [13]: 1 = normal with very few inflammatory cells bordering the arteries or airways; 2 = scattered inflammatory cells surrounding the artery or airway up to two rings in depth; 3 = cell cuffs or clusters of inflammatory cells surrounding the artery or airway three rings or more in depth. b. Arterial remodeling in offspring. Offspring from mothers treated during pregnancy as outlined in Fig. 1 were exposed to A. fumigatus intranasally with 5-6 doses starting at 9-10 weeks of age. Arterial remodeling was scored as described [13] and in Figure 3b. Significant differences across groups were not detected. p = 0.183 on ANOVA. Arterial remodeling was scored as follows [13]: 1 = normal; 2 = thickened vascular wall with intact lumen and circular media; 3 = obstructed lumen and thickened wall lined with disorganized layers of cells.

Mentions: However, using an established semi-quantitative scoring system [13], only a nonsignificant trend in airway inflammation was observed among mice that were treated with A. fumigatus when compared to mice whose mothers were treated with saline alone (1.84 ± 3.74 saline vs. 2.05 ± 0.07 A. fumigatus mean airway inflammation score ± SE, p = 0.11 ANOVA)(Figure 6a). Also, we were unable to detect a correlation between total airway inflammation scores and IgE levels measured at any of the 3 time points (R-value = 0.096 after the third dose, 0.016 after the fifth dose and -0.077 after the sixth dose, p = NS for each) (Figure 6b). Correlations between inflammation score and IgG1 (R-value = 0.228 after the fifth dose, 0.222 after the sixth dose, p = NS for each) were not detected either.


Prenatal allergen and diesel exhaust exposure and their effects on allergy in adult offspring mice.

Corson L, Zhu H, Quan C, Grunig G, Ballaney M, Jin X, Perera FP, Factor PH, Chen LC, Miller RL - Allergy Asthma Clin Immunol (2010)

Perivascular, peribronchial airway inflammation and airway remodeling in offspring. a. Perivascular, peribronchial airway inflammation in offspring of A. fumigatus. Composite scores were obtained following 5 or 6 doses of A. fumigatus. Significant differences across groups were not detected. p = 0.11 on ANOVA. Perivascular and peribronchial inflammation were scored as follows [13]: 1 = normal with very few inflammatory cells bordering the arteries or airways; 2 = scattered inflammatory cells surrounding the artery or airway up to two rings in depth; 3 = cell cuffs or clusters of inflammatory cells surrounding the artery or airway three rings or more in depth. b. Arterial remodeling in offspring. Offspring from mothers treated during pregnancy as outlined in Fig. 1 were exposed to A. fumigatus intranasally with 5-6 doses starting at 9-10 weeks of age. Arterial remodeling was scored as described [13] and in Figure 3b. Significant differences across groups were not detected. p = 0.183 on ANOVA. Arterial remodeling was scored as follows [13]: 1 = normal; 2 = thickened vascular wall with intact lumen and circular media; 3 = obstructed lumen and thickened wall lined with disorganized layers of cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2875211&req=5

Figure 6: Perivascular, peribronchial airway inflammation and airway remodeling in offspring. a. Perivascular, peribronchial airway inflammation in offspring of A. fumigatus. Composite scores were obtained following 5 or 6 doses of A. fumigatus. Significant differences across groups were not detected. p = 0.11 on ANOVA. Perivascular and peribronchial inflammation were scored as follows [13]: 1 = normal with very few inflammatory cells bordering the arteries or airways; 2 = scattered inflammatory cells surrounding the artery or airway up to two rings in depth; 3 = cell cuffs or clusters of inflammatory cells surrounding the artery or airway three rings or more in depth. b. Arterial remodeling in offspring. Offspring from mothers treated during pregnancy as outlined in Fig. 1 were exposed to A. fumigatus intranasally with 5-6 doses starting at 9-10 weeks of age. Arterial remodeling was scored as described [13] and in Figure 3b. Significant differences across groups were not detected. p = 0.183 on ANOVA. Arterial remodeling was scored as follows [13]: 1 = normal; 2 = thickened vascular wall with intact lumen and circular media; 3 = obstructed lumen and thickened wall lined with disorganized layers of cells.
Mentions: However, using an established semi-quantitative scoring system [13], only a nonsignificant trend in airway inflammation was observed among mice that were treated with A. fumigatus when compared to mice whose mothers were treated with saline alone (1.84 ± 3.74 saline vs. 2.05 ± 0.07 A. fumigatus mean airway inflammation score ± SE, p = 0.11 ANOVA)(Figure 6a). Also, we were unable to detect a correlation between total airway inflammation scores and IgE levels measured at any of the 3 time points (R-value = 0.096 after the third dose, 0.016 after the fifth dose and -0.077 after the sixth dose, p = NS for each) (Figure 6b). Correlations between inflammation score and IgG1 (R-value = 0.228 after the fifth dose, 0.222 after the sixth dose, p = NS for each) were not detected either.

Bottom Line: However, the effects of prenatal environmental exposures on adult offspring have not been well-studied.At age 9-10 weeks, their offspring were sensitized and challenged with A. fumigatus.These results suggest that, in this model, allergen and/or diesel administration during pregnancy may be associated with protection from developing systemic and airway allergic immune responses in the adult offspring.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA. rlm14@columbia.edu

ABSTRACT

Background: Multiple studies have suggested that prenatal exposure to either allergens or air pollution may increase the risk for the development of allergic immune responses in young offspring. However, the effects of prenatal environmental exposures on adult offspring have not been well-studied. We hypothesized that combined prenatal exposure to Aspergillus fumigatus (A. fumigatus) allergen and diesel exhaust particles will be associated with altered IgE production, airway inflammation, airway hyperreactivity (AHR), and airway remodeling of adult offspring.

Methods: Following sensitization via the airway route to A. fumigatus and mating, pregnant BALB/c mice were exposed to additional A. fumigatus and/or diesel exhaust particles. At age 9-10 weeks, their offspring were sensitized and challenged with A. fumigatus.

Results: We found that adult offspring from mice that were exposed to A. fumigatus or diesel exhaust particles during pregnancy experienced decreases in IgE production. Adult offspring of mice that were exposed to both A. fumigatus and diesel exhaust particles during pregnancy experienced decreases in airway eosinophilia.

Conclusion: These results suggest that, in this model, allergen and/or diesel administration during pregnancy may be associated with protection from developing systemic and airway allergic immune responses in the adult offspring.

No MeSH data available.


Related in: MedlinePlus