Limits...
Prenatal allergen and diesel exhaust exposure and their effects on allergy in adult offspring mice.

Corson L, Zhu H, Quan C, Grunig G, Ballaney M, Jin X, Perera FP, Factor PH, Chen LC, Miller RL - Allergy Asthma Clin Immunol (2010)

Bottom Line: However, the effects of prenatal environmental exposures on adult offspring have not been well-studied.At age 9-10 weeks, their offspring were sensitized and challenged with A. fumigatus.These results suggest that, in this model, allergen and/or diesel administration during pregnancy may be associated with protection from developing systemic and airway allergic immune responses in the adult offspring.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA. rlm14@columbia.edu

ABSTRACT

Background: Multiple studies have suggested that prenatal exposure to either allergens or air pollution may increase the risk for the development of allergic immune responses in young offspring. However, the effects of prenatal environmental exposures on adult offspring have not been well-studied. We hypothesized that combined prenatal exposure to Aspergillus fumigatus (A. fumigatus) allergen and diesel exhaust particles will be associated with altered IgE production, airway inflammation, airway hyperreactivity (AHR), and airway remodeling of adult offspring.

Methods: Following sensitization via the airway route to A. fumigatus and mating, pregnant BALB/c mice were exposed to additional A. fumigatus and/or diesel exhaust particles. At age 9-10 weeks, their offspring were sensitized and challenged with A. fumigatus.

Results: We found that adult offspring from mice that were exposed to A. fumigatus or diesel exhaust particles during pregnancy experienced decreases in IgE production. Adult offspring of mice that were exposed to both A. fumigatus and diesel exhaust particles during pregnancy experienced decreases in airway eosinophilia.

Conclusion: These results suggest that, in this model, allergen and/or diesel administration during pregnancy may be associated with protection from developing systemic and airway allergic immune responses in the adult offspring.

No MeSH data available.


Related in: MedlinePlus

Differential airway cell counts in offspring after five and six doses of A. fumigatus. Eosinophil counts were significantly decreased (and macrophages significantly increased) among offspring from mothers following diesel exhaust and A. fumigatus compared to offspring of mothers treated with saline alone, * p < 0.0002 on ANOVA and p < 0.05 by Tukey HSD or with A. fumigatus alone, † p < 0.0003 on ANOVA and p < 0.01 by Tukey HSD.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2875211&req=5

Figure 4: Differential airway cell counts in offspring after five and six doses of A. fumigatus. Eosinophil counts were significantly decreased (and macrophages significantly increased) among offspring from mothers following diesel exhaust and A. fumigatus compared to offspring of mothers treated with saline alone, * p < 0.0002 on ANOVA and p < 0.05 by Tukey HSD or with A. fumigatus alone, † p < 0.0003 on ANOVA and p < 0.01 by Tukey HSD.

Mentions: Adult offspring from mothers that received both A. fumigatus and DEP developed significantly less airway eosinophilia (mean eosinophil count 13.24 ± 2.04%) compared to offspring from mothers that had received A. fumigatus (26.44 ± 2.89%, p = 0.01, Tukey HSD) or saline (23.83 ± 3.33%, p = 0.05, Tukey HSD) alone. The first result (A. fumigatus and DEP lower than A. fumigatus) was replicated when examining absolute numbers of eosinophils (p < 0.001 on ANOVA and p < 0.01 by Tukey HSD). Adult offspring from mothers that received both A. fumigatus and DEP also developed higher levels of macrophage counts compared to offspring of mothers that had received A. fumigatus (p = 0.01, Tukey HSD) or saline (p = 0.05, Tukey HSD) alone (Figure 4). Airway eosinophil counts did not correlate with IgE levels measured at any of the time points (Spearman rank correlation R-value = -0.055 after the third dose, 0.019 after the fifth dose and -0.082 after the sixth dose, p = nonsignificant (NS) for each). Airway eosinophil counts also did not correlate with IgG1 levels after the fifth (R-value = 0.216, p = NS) or sixth dose (R-value = -0.185, p = NS).


Prenatal allergen and diesel exhaust exposure and their effects on allergy in adult offspring mice.

Corson L, Zhu H, Quan C, Grunig G, Ballaney M, Jin X, Perera FP, Factor PH, Chen LC, Miller RL - Allergy Asthma Clin Immunol (2010)

Differential airway cell counts in offspring after five and six doses of A. fumigatus. Eosinophil counts were significantly decreased (and macrophages significantly increased) among offspring from mothers following diesel exhaust and A. fumigatus compared to offspring of mothers treated with saline alone, * p < 0.0002 on ANOVA and p < 0.05 by Tukey HSD or with A. fumigatus alone, † p < 0.0003 on ANOVA and p < 0.01 by Tukey HSD.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2875211&req=5

Figure 4: Differential airway cell counts in offspring after five and six doses of A. fumigatus. Eosinophil counts were significantly decreased (and macrophages significantly increased) among offspring from mothers following diesel exhaust and A. fumigatus compared to offspring of mothers treated with saline alone, * p < 0.0002 on ANOVA and p < 0.05 by Tukey HSD or with A. fumigatus alone, † p < 0.0003 on ANOVA and p < 0.01 by Tukey HSD.
Mentions: Adult offspring from mothers that received both A. fumigatus and DEP developed significantly less airway eosinophilia (mean eosinophil count 13.24 ± 2.04%) compared to offspring from mothers that had received A. fumigatus (26.44 ± 2.89%, p = 0.01, Tukey HSD) or saline (23.83 ± 3.33%, p = 0.05, Tukey HSD) alone. The first result (A. fumigatus and DEP lower than A. fumigatus) was replicated when examining absolute numbers of eosinophils (p < 0.001 on ANOVA and p < 0.01 by Tukey HSD). Adult offspring from mothers that received both A. fumigatus and DEP also developed higher levels of macrophage counts compared to offspring of mothers that had received A. fumigatus (p = 0.01, Tukey HSD) or saline (p = 0.05, Tukey HSD) alone (Figure 4). Airway eosinophil counts did not correlate with IgE levels measured at any of the time points (Spearman rank correlation R-value = -0.055 after the third dose, 0.019 after the fifth dose and -0.082 after the sixth dose, p = nonsignificant (NS) for each). Airway eosinophil counts also did not correlate with IgG1 levels after the fifth (R-value = 0.216, p = NS) or sixth dose (R-value = -0.185, p = NS).

Bottom Line: However, the effects of prenatal environmental exposures on adult offspring have not been well-studied.At age 9-10 weeks, their offspring were sensitized and challenged with A. fumigatus.These results suggest that, in this model, allergen and/or diesel administration during pregnancy may be associated with protection from developing systemic and airway allergic immune responses in the adult offspring.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA. rlm14@columbia.edu

ABSTRACT

Background: Multiple studies have suggested that prenatal exposure to either allergens or air pollution may increase the risk for the development of allergic immune responses in young offspring. However, the effects of prenatal environmental exposures on adult offspring have not been well-studied. We hypothesized that combined prenatal exposure to Aspergillus fumigatus (A. fumigatus) allergen and diesel exhaust particles will be associated with altered IgE production, airway inflammation, airway hyperreactivity (AHR), and airway remodeling of adult offspring.

Methods: Following sensitization via the airway route to A. fumigatus and mating, pregnant BALB/c mice were exposed to additional A. fumigatus and/or diesel exhaust particles. At age 9-10 weeks, their offspring were sensitized and challenged with A. fumigatus.

Results: We found that adult offspring from mice that were exposed to A. fumigatus or diesel exhaust particles during pregnancy experienced decreases in IgE production. Adult offspring of mice that were exposed to both A. fumigatus and diesel exhaust particles during pregnancy experienced decreases in airway eosinophilia.

Conclusion: These results suggest that, in this model, allergen and/or diesel administration during pregnancy may be associated with protection from developing systemic and airway allergic immune responses in the adult offspring.

No MeSH data available.


Related in: MedlinePlus