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Prenatal allergen and diesel exhaust exposure and their effects on allergy in adult offspring mice.

Corson L, Zhu H, Quan C, Grunig G, Ballaney M, Jin X, Perera FP, Factor PH, Chen LC, Miller RL - Allergy Asthma Clin Immunol (2010)

Bottom Line: However, the effects of prenatal environmental exposures on adult offspring have not been well-studied.At age 9-10 weeks, their offspring were sensitized and challenged with A. fumigatus.These results suggest that, in this model, allergen and/or diesel administration during pregnancy may be associated with protection from developing systemic and airway allergic immune responses in the adult offspring.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA. rlm14@columbia.edu

ABSTRACT

Background: Multiple studies have suggested that prenatal exposure to either allergens or air pollution may increase the risk for the development of allergic immune responses in young offspring. However, the effects of prenatal environmental exposures on adult offspring have not been well-studied. We hypothesized that combined prenatal exposure to Aspergillus fumigatus (A. fumigatus) allergen and diesel exhaust particles will be associated with altered IgE production, airway inflammation, airway hyperreactivity (AHR), and airway remodeling of adult offspring.

Methods: Following sensitization via the airway route to A. fumigatus and mating, pregnant BALB/c mice were exposed to additional A. fumigatus and/or diesel exhaust particles. At age 9-10 weeks, their offspring were sensitized and challenged with A. fumigatus.

Results: We found that adult offspring from mice that were exposed to A. fumigatus or diesel exhaust particles during pregnancy experienced decreases in IgE production. Adult offspring of mice that were exposed to both A. fumigatus and diesel exhaust particles during pregnancy experienced decreases in airway eosinophilia.

Conclusion: These results suggest that, in this model, allergen and/or diesel administration during pregnancy may be associated with protection from developing systemic and airway allergic immune responses in the adult offspring.

No MeSH data available.


Related in: MedlinePlus

Ig induction in offspring after three, five and six doses of A. fumigatus. a) IgE was reduced after the fifth and sixth (p < 0.0001 on ANOVA), but not third (p = NS, ANOVA), doses among offspring mice whose mothers were exposed to either A. fumigatus or diesel exhaust particles or both. *p < 0.01, when compared to saline alone by Tukey HSD. † p < 0.05, when compared to A. fumigatus alone by Tukey HSD b) IgG1 was greater after the fifth, sixth (p < 0.0001 on ANOVA), but not third (p = NS, ANOVA), doses among mice whose mothers were exposed to either A. fumigatus or diesel exhaust particles or both. *p < 0.01, when compared to saline alone by Tukey HSD. †p < 0.05, when compared to saline alone by Tukey HSD. c) IgG2a was greater after the fifth, sixth (p < 0.0001 on ANOVA) dose among mice whose mothers were exposed to A. fumigatus or diesel exhaust particles plus A. fumigatus. *p < 0.01, when compared to saline alone by Tukey HSD. Levels also were greater among offspring of mothers that were exposed to both diesel exhaust particles and A. fumigatus when compared to offspring of mothers treated either with diesel exhaust or A. fumigatus alone, p < 0.01.†p < 0.01, when compared to diesel exhaust particles alone by Tukey HSD. ‡ p < 0.05 on ANOVA and when compared to saline alone by Tukey HSD. Sample sizes corresponding to the figures vary as follows: Saline 11-14; Diesel 11-15; A. fumigatus: 8-18; Diesel and A. fumigatus 13-25.
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Figure 3: Ig induction in offspring after three, five and six doses of A. fumigatus. a) IgE was reduced after the fifth and sixth (p < 0.0001 on ANOVA), but not third (p = NS, ANOVA), doses among offspring mice whose mothers were exposed to either A. fumigatus or diesel exhaust particles or both. *p < 0.01, when compared to saline alone by Tukey HSD. † p < 0.05, when compared to A. fumigatus alone by Tukey HSD b) IgG1 was greater after the fifth, sixth (p < 0.0001 on ANOVA), but not third (p = NS, ANOVA), doses among mice whose mothers were exposed to either A. fumigatus or diesel exhaust particles or both. *p < 0.01, when compared to saline alone by Tukey HSD. †p < 0.05, when compared to saline alone by Tukey HSD. c) IgG2a was greater after the fifth, sixth (p < 0.0001 on ANOVA) dose among mice whose mothers were exposed to A. fumigatus or diesel exhaust particles plus A. fumigatus. *p < 0.01, when compared to saline alone by Tukey HSD. Levels also were greater among offspring of mothers that were exposed to both diesel exhaust particles and A. fumigatus when compared to offspring of mothers treated either with diesel exhaust or A. fumigatus alone, p < 0.01.†p < 0.01, when compared to diesel exhaust particles alone by Tukey HSD. ‡ p < 0.05 on ANOVA and when compared to saline alone by Tukey HSD. Sample sizes corresponding to the figures vary as follows: Saline 11-14; Diesel 11-15; A. fumigatus: 8-18; Diesel and A. fumigatus 13-25.

Mentions: Adult offspring from mothers who received A. fumigatus or DEP alone, or A. fumigatus and DEP together, developed lower levels of total IgE when assessed after the fifth dose of A. fumigatus compared to offspring from mothers treated with saline only prior to mating (p < 0.0001 ANOVA, Figure 3a). In addition, IgE levels from offspring from mice exposed to DEP alone were lower than those from offspring from mice exposed to A. fumigatus alone (p < 0.05 Tukey HSD test). Adult offspring from mothers who received A. fumigatus or DEP alone, or DEP and A. fumigatus together, developed lower IgE levels compared with levels from offspring whose mothers received saline alone when assessed after the sixth dose of allergen treatment as well (p < 0.0001 ANOVA). In addition, IgE levels from adult offspring of mice that were treated with DEP and A. fumigatus were lower than those from offspring of mice that were treated with A. fumigatus alone (p < 0.05, Tukey HSD test). Significant differences in IgE levels were not apparent after the third dose of A. fumigatus


Prenatal allergen and diesel exhaust exposure and their effects on allergy in adult offspring mice.

Corson L, Zhu H, Quan C, Grunig G, Ballaney M, Jin X, Perera FP, Factor PH, Chen LC, Miller RL - Allergy Asthma Clin Immunol (2010)

Ig induction in offspring after three, five and six doses of A. fumigatus. a) IgE was reduced after the fifth and sixth (p < 0.0001 on ANOVA), but not third (p = NS, ANOVA), doses among offspring mice whose mothers were exposed to either A. fumigatus or diesel exhaust particles or both. *p < 0.01, when compared to saline alone by Tukey HSD. † p < 0.05, when compared to A. fumigatus alone by Tukey HSD b) IgG1 was greater after the fifth, sixth (p < 0.0001 on ANOVA), but not third (p = NS, ANOVA), doses among mice whose mothers were exposed to either A. fumigatus or diesel exhaust particles or both. *p < 0.01, when compared to saline alone by Tukey HSD. †p < 0.05, when compared to saline alone by Tukey HSD. c) IgG2a was greater after the fifth, sixth (p < 0.0001 on ANOVA) dose among mice whose mothers were exposed to A. fumigatus or diesel exhaust particles plus A. fumigatus. *p < 0.01, when compared to saline alone by Tukey HSD. Levels also were greater among offspring of mothers that were exposed to both diesel exhaust particles and A. fumigatus when compared to offspring of mothers treated either with diesel exhaust or A. fumigatus alone, p < 0.01.†p < 0.01, when compared to diesel exhaust particles alone by Tukey HSD. ‡ p < 0.05 on ANOVA and when compared to saline alone by Tukey HSD. Sample sizes corresponding to the figures vary as follows: Saline 11-14; Diesel 11-15; A. fumigatus: 8-18; Diesel and A. fumigatus 13-25.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
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getmorefigures.php?uid=PMC2875211&req=5

Figure 3: Ig induction in offspring after three, five and six doses of A. fumigatus. a) IgE was reduced after the fifth and sixth (p < 0.0001 on ANOVA), but not third (p = NS, ANOVA), doses among offspring mice whose mothers were exposed to either A. fumigatus or diesel exhaust particles or both. *p < 0.01, when compared to saline alone by Tukey HSD. † p < 0.05, when compared to A. fumigatus alone by Tukey HSD b) IgG1 was greater after the fifth, sixth (p < 0.0001 on ANOVA), but not third (p = NS, ANOVA), doses among mice whose mothers were exposed to either A. fumigatus or diesel exhaust particles or both. *p < 0.01, when compared to saline alone by Tukey HSD. †p < 0.05, when compared to saline alone by Tukey HSD. c) IgG2a was greater after the fifth, sixth (p < 0.0001 on ANOVA) dose among mice whose mothers were exposed to A. fumigatus or diesel exhaust particles plus A. fumigatus. *p < 0.01, when compared to saline alone by Tukey HSD. Levels also were greater among offspring of mothers that were exposed to both diesel exhaust particles and A. fumigatus when compared to offspring of mothers treated either with diesel exhaust or A. fumigatus alone, p < 0.01.†p < 0.01, when compared to diesel exhaust particles alone by Tukey HSD. ‡ p < 0.05 on ANOVA and when compared to saline alone by Tukey HSD. Sample sizes corresponding to the figures vary as follows: Saline 11-14; Diesel 11-15; A. fumigatus: 8-18; Diesel and A. fumigatus 13-25.
Mentions: Adult offspring from mothers who received A. fumigatus or DEP alone, or A. fumigatus and DEP together, developed lower levels of total IgE when assessed after the fifth dose of A. fumigatus compared to offspring from mothers treated with saline only prior to mating (p < 0.0001 ANOVA, Figure 3a). In addition, IgE levels from offspring from mice exposed to DEP alone were lower than those from offspring from mice exposed to A. fumigatus alone (p < 0.05 Tukey HSD test). Adult offspring from mothers who received A. fumigatus or DEP alone, or DEP and A. fumigatus together, developed lower IgE levels compared with levels from offspring whose mothers received saline alone when assessed after the sixth dose of allergen treatment as well (p < 0.0001 ANOVA). In addition, IgE levels from adult offspring of mice that were treated with DEP and A. fumigatus were lower than those from offspring of mice that were treated with A. fumigatus alone (p < 0.05, Tukey HSD test). Significant differences in IgE levels were not apparent after the third dose of A. fumigatus

Bottom Line: However, the effects of prenatal environmental exposures on adult offspring have not been well-studied.At age 9-10 weeks, their offspring were sensitized and challenged with A. fumigatus.These results suggest that, in this model, allergen and/or diesel administration during pregnancy may be associated with protection from developing systemic and airway allergic immune responses in the adult offspring.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA. rlm14@columbia.edu

ABSTRACT

Background: Multiple studies have suggested that prenatal exposure to either allergens or air pollution may increase the risk for the development of allergic immune responses in young offspring. However, the effects of prenatal environmental exposures on adult offspring have not been well-studied. We hypothesized that combined prenatal exposure to Aspergillus fumigatus (A. fumigatus) allergen and diesel exhaust particles will be associated with altered IgE production, airway inflammation, airway hyperreactivity (AHR), and airway remodeling of adult offspring.

Methods: Following sensitization via the airway route to A. fumigatus and mating, pregnant BALB/c mice were exposed to additional A. fumigatus and/or diesel exhaust particles. At age 9-10 weeks, their offspring were sensitized and challenged with A. fumigatus.

Results: We found that adult offspring from mice that were exposed to A. fumigatus or diesel exhaust particles during pregnancy experienced decreases in IgE production. Adult offspring of mice that were exposed to both A. fumigatus and diesel exhaust particles during pregnancy experienced decreases in airway eosinophilia.

Conclusion: These results suggest that, in this model, allergen and/or diesel administration during pregnancy may be associated with protection from developing systemic and airway allergic immune responses in the adult offspring.

No MeSH data available.


Related in: MedlinePlus