Limits...
Prenatal allergen and diesel exhaust exposure and their effects on allergy in adult offspring mice.

Corson L, Zhu H, Quan C, Grunig G, Ballaney M, Jin X, Perera FP, Factor PH, Chen LC, Miller RL - Allergy Asthma Clin Immunol (2010)

Bottom Line: However, the effects of prenatal environmental exposures on adult offspring have not been well-studied.At age 9-10 weeks, their offspring were sensitized and challenged with A. fumigatus.These results suggest that, in this model, allergen and/or diesel administration during pregnancy may be associated with protection from developing systemic and airway allergic immune responses in the adult offspring.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA. rlm14@columbia.edu

ABSTRACT

Background: Multiple studies have suggested that prenatal exposure to either allergens or air pollution may increase the risk for the development of allergic immune responses in young offspring. However, the effects of prenatal environmental exposures on adult offspring have not been well-studied. We hypothesized that combined prenatal exposure to Aspergillus fumigatus (A. fumigatus) allergen and diesel exhaust particles will be associated with altered IgE production, airway inflammation, airway hyperreactivity (AHR), and airway remodeling of adult offspring.

Methods: Following sensitization via the airway route to A. fumigatus and mating, pregnant BALB/c mice were exposed to additional A. fumigatus and/or diesel exhaust particles. At age 9-10 weeks, their offspring were sensitized and challenged with A. fumigatus.

Results: We found that adult offspring from mice that were exposed to A. fumigatus or diesel exhaust particles during pregnancy experienced decreases in IgE production. Adult offspring of mice that were exposed to both A. fumigatus and diesel exhaust particles during pregnancy experienced decreases in airway eosinophilia.

Conclusion: These results suggest that, in this model, allergen and/or diesel administration during pregnancy may be associated with protection from developing systemic and airway allergic immune responses in the adult offspring.

No MeSH data available.


Related in: MedlinePlus

Experimental protocol. Adult females received 5 dosages of A. fumigatus or saline, 20, 16, 12, 8, and 4 days prior to mating. During the second and third weeks of pregnancy, mothers received diesel exhaust particle exposure Monday through Friday plus A. fumigatus or saline on days 7 and 14. AHR: Airway hyperreactivity BAL: Bronchoalveolar lavage i.n: intranasal 3×: 3 doses of A. fumigatus 5×: 5 doses of A. fumigatus 6×: 6 doses of A. fumigatus
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2875211&req=5

Figure 1: Experimental protocol. Adult females received 5 dosages of A. fumigatus or saline, 20, 16, 12, 8, and 4 days prior to mating. During the second and third weeks of pregnancy, mothers received diesel exhaust particle exposure Monday through Friday plus A. fumigatus or saline on days 7 and 14. AHR: Airway hyperreactivity BAL: Bronchoalveolar lavage i.n: intranasal 3×: 3 doses of A. fumigatus 5×: 5 doses of A. fumigatus 6×: 6 doses of A. fumigatus

Mentions: Six week old wild-type female and male BALB/c mice were obtained from Jackson Laboratories (Bar Harbor, ME). Males and females were housed separately prior to mating. All animals were housed at New York University (NYU) animal facility (Tuxedo, NY) and fed a commercial pellet mouse feed. Mice were lightly anesthetized with isoflurane (2% inhaled). Intranasal application of A. fumigatus (62.5 ug) (Hollister-Stier Co., Spokane, WA; measured endotoxin dose < 0.16 EU/ml: Endotoxin Testing Service, Cambrex Bio Science Walkersville, Inc, MD) in 50 ul of saline or saline vehicle alone was administered five times, four days apart, beginning 20 days prior to mating. Pregnant mice were treated again with A. fumigatus or saline on day 7 and 14 after mating. Offspring were separated from their mothers at 21 days of age. At 9-10 weeks of age, all offspring were treated with either five or six dosages of A. fumigatus each dose four days apart (Figure 1). All experimental procedures were approved by IACUCs at Columbia University and New York University.


Prenatal allergen and diesel exhaust exposure and their effects on allergy in adult offspring mice.

Corson L, Zhu H, Quan C, Grunig G, Ballaney M, Jin X, Perera FP, Factor PH, Chen LC, Miller RL - Allergy Asthma Clin Immunol (2010)

Experimental protocol. Adult females received 5 dosages of A. fumigatus or saline, 20, 16, 12, 8, and 4 days prior to mating. During the second and third weeks of pregnancy, mothers received diesel exhaust particle exposure Monday through Friday plus A. fumigatus or saline on days 7 and 14. AHR: Airway hyperreactivity BAL: Bronchoalveolar lavage i.n: intranasal 3×: 3 doses of A. fumigatus 5×: 5 doses of A. fumigatus 6×: 6 doses of A. fumigatus
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2875211&req=5

Figure 1: Experimental protocol. Adult females received 5 dosages of A. fumigatus or saline, 20, 16, 12, 8, and 4 days prior to mating. During the second and third weeks of pregnancy, mothers received diesel exhaust particle exposure Monday through Friday plus A. fumigatus or saline on days 7 and 14. AHR: Airway hyperreactivity BAL: Bronchoalveolar lavage i.n: intranasal 3×: 3 doses of A. fumigatus 5×: 5 doses of A. fumigatus 6×: 6 doses of A. fumigatus
Mentions: Six week old wild-type female and male BALB/c mice were obtained from Jackson Laboratories (Bar Harbor, ME). Males and females were housed separately prior to mating. All animals were housed at New York University (NYU) animal facility (Tuxedo, NY) and fed a commercial pellet mouse feed. Mice were lightly anesthetized with isoflurane (2% inhaled). Intranasal application of A. fumigatus (62.5 ug) (Hollister-Stier Co., Spokane, WA; measured endotoxin dose < 0.16 EU/ml: Endotoxin Testing Service, Cambrex Bio Science Walkersville, Inc, MD) in 50 ul of saline or saline vehicle alone was administered five times, four days apart, beginning 20 days prior to mating. Pregnant mice were treated again with A. fumigatus or saline on day 7 and 14 after mating. Offspring were separated from their mothers at 21 days of age. At 9-10 weeks of age, all offspring were treated with either five or six dosages of A. fumigatus each dose four days apart (Figure 1). All experimental procedures were approved by IACUCs at Columbia University and New York University.

Bottom Line: However, the effects of prenatal environmental exposures on adult offspring have not been well-studied.At age 9-10 weeks, their offspring were sensitized and challenged with A. fumigatus.These results suggest that, in this model, allergen and/or diesel administration during pregnancy may be associated with protection from developing systemic and airway allergic immune responses in the adult offspring.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA. rlm14@columbia.edu

ABSTRACT

Background: Multiple studies have suggested that prenatal exposure to either allergens or air pollution may increase the risk for the development of allergic immune responses in young offspring. However, the effects of prenatal environmental exposures on adult offspring have not been well-studied. We hypothesized that combined prenatal exposure to Aspergillus fumigatus (A. fumigatus) allergen and diesel exhaust particles will be associated with altered IgE production, airway inflammation, airway hyperreactivity (AHR), and airway remodeling of adult offspring.

Methods: Following sensitization via the airway route to A. fumigatus and mating, pregnant BALB/c mice were exposed to additional A. fumigatus and/or diesel exhaust particles. At age 9-10 weeks, their offspring were sensitized and challenged with A. fumigatus.

Results: We found that adult offspring from mice that were exposed to A. fumigatus or diesel exhaust particles during pregnancy experienced decreases in IgE production. Adult offspring of mice that were exposed to both A. fumigatus and diesel exhaust particles during pregnancy experienced decreases in airway eosinophilia.

Conclusion: These results suggest that, in this model, allergen and/or diesel administration during pregnancy may be associated with protection from developing systemic and airway allergic immune responses in the adult offspring.

No MeSH data available.


Related in: MedlinePlus