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Optimization of the fermentation process of actinomycete strain hhs.015.

Wang X, Huang L, Kang Z, Buchenauer H, Gao X - J. Biomed. Biotechnol. (2010)

Bottom Line: The results showed that the alternative medium contained 15 g apple pomace, 4 g rapeseed meal, 0.1 g KH(2)PO(4), and 0.6 g MgSO(4).7H(2)O in 1 L distilled water.This medium reduced the material costs by 91.5% compared to SSY medium.The antimicrobial activity was increased by 20% by optimizing the environmental parameters.

View Article: PubMed Central - PubMed

Affiliation: College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100, China.

ABSTRACT
Strain Hhs.015(T) (Saccharothrix yanglingensis sp. nov.), an antagonistic endophytic Saccharothrix actinomycete isolated from roots of cucumber plants, exhibited a broad antimicrobial spectrum in vitro and was active as a biocontrol against plant diseases in field trials. The SSY medium was used for production of antimicrobial metabolites by strain Hhs.015(T). However, this medium is too expensive for large-scale production. In this study, an alternative culture medium, based on agricultural waste products (e.g., apple pomace), was optimized. The results showed that the alternative medium contained 15 g apple pomace, 4 g rapeseed meal, 0.1 g KH(2)PO(4), and 0.6 g MgSO(4).7H(2)O in 1 L distilled water. This medium reduced the material costs by 91.5% compared to SSY medium. Response surface methodology (RSM) was used to investigate the influence of environmental variables on production of compounds of antimicrobial metabolites. The optimal conditions achieved were initial pH 7.0, medium volume of 90 mL in 250 mL flasks, rotary speed of 100 rpm, temperature 25 degrees C, and inoculation volume of 15.8%. The antimicrobial activity was increased by 20% by optimizing the environmental parameters. The results obtained allow an efficient production of components with antimicrobial activity by strain Hhs.015(T) on a large scale at low costs.

No MeSH data available.


Related in: MedlinePlus

The antimicrobial activity in fermentation supernatants of strain Hhs.015T to Candida vulgaris on PDA medium in 9-cm-diameter Petri dish. The antimicrobial activity was determined by measuring the translucent inhibition zones after incubating the dish 24 hours at 30°C. A: alternative culture medium at basal fermentation condition; B: alternative culture medium at the optimal fermentation condition; CK: SSY medium at the basal fermentation condition.
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fig2: The antimicrobial activity in fermentation supernatants of strain Hhs.015T to Candida vulgaris on PDA medium in 9-cm-diameter Petri dish. The antimicrobial activity was determined by measuring the translucent inhibition zones after incubating the dish 24 hours at 30°C. A: alternative culture medium at basal fermentation condition; B: alternative culture medium at the optimal fermentation condition; CK: SSY medium at the basal fermentation condition.

Mentions: The effects of concentrations of the components in the medium on the antimicrobial activity were further investigated by tests of orthogonal design. From the range analysis, the apple pomace as the main variable affected the antimicrobial activity, indicating that the carbon source was essential for the production of compounds with antimicrobial activity by strain Hhs.015T. From the results of the analysis of variance of orthogonal design, (Table 7) showed that each single variable had no significant effect on the antimicrobial activity (F < F0.05), probably because in the experiments the concentration range of every variable was defined. Finally, the optimum values of apple pomace 15 g/L, rapeseed meal 4 g/L, KH2PO4 0.1 g/L, and MgSO4·7H2O 0.6 g/L were determined according to the range analysis (Table 6). The antimicrobial activity produced in the alternative culture medium was not significantly different from the SSY medium (Figure 2A, CK), while the price of the materials of 0.1836 RMB/L was drastically lower than the price of the SSY medium, 2.1624 RMB/L; thus, the price was reduced by 91.5%.


Optimization of the fermentation process of actinomycete strain hhs.015.

Wang X, Huang L, Kang Z, Buchenauer H, Gao X - J. Biomed. Biotechnol. (2010)

The antimicrobial activity in fermentation supernatants of strain Hhs.015T to Candida vulgaris on PDA medium in 9-cm-diameter Petri dish. The antimicrobial activity was determined by measuring the translucent inhibition zones after incubating the dish 24 hours at 30°C. A: alternative culture medium at basal fermentation condition; B: alternative culture medium at the optimal fermentation condition; CK: SSY medium at the basal fermentation condition.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2874929&req=5

fig2: The antimicrobial activity in fermentation supernatants of strain Hhs.015T to Candida vulgaris on PDA medium in 9-cm-diameter Petri dish. The antimicrobial activity was determined by measuring the translucent inhibition zones after incubating the dish 24 hours at 30°C. A: alternative culture medium at basal fermentation condition; B: alternative culture medium at the optimal fermentation condition; CK: SSY medium at the basal fermentation condition.
Mentions: The effects of concentrations of the components in the medium on the antimicrobial activity were further investigated by tests of orthogonal design. From the range analysis, the apple pomace as the main variable affected the antimicrobial activity, indicating that the carbon source was essential for the production of compounds with antimicrobial activity by strain Hhs.015T. From the results of the analysis of variance of orthogonal design, (Table 7) showed that each single variable had no significant effect on the antimicrobial activity (F < F0.05), probably because in the experiments the concentration range of every variable was defined. Finally, the optimum values of apple pomace 15 g/L, rapeseed meal 4 g/L, KH2PO4 0.1 g/L, and MgSO4·7H2O 0.6 g/L were determined according to the range analysis (Table 6). The antimicrobial activity produced in the alternative culture medium was not significantly different from the SSY medium (Figure 2A, CK), while the price of the materials of 0.1836 RMB/L was drastically lower than the price of the SSY medium, 2.1624 RMB/L; thus, the price was reduced by 91.5%.

Bottom Line: The results showed that the alternative medium contained 15 g apple pomace, 4 g rapeseed meal, 0.1 g KH(2)PO(4), and 0.6 g MgSO(4).7H(2)O in 1 L distilled water.This medium reduced the material costs by 91.5% compared to SSY medium.The antimicrobial activity was increased by 20% by optimizing the environmental parameters.

View Article: PubMed Central - PubMed

Affiliation: College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100, China.

ABSTRACT
Strain Hhs.015(T) (Saccharothrix yanglingensis sp. nov.), an antagonistic endophytic Saccharothrix actinomycete isolated from roots of cucumber plants, exhibited a broad antimicrobial spectrum in vitro and was active as a biocontrol against plant diseases in field trials. The SSY medium was used for production of antimicrobial metabolites by strain Hhs.015(T). However, this medium is too expensive for large-scale production. In this study, an alternative culture medium, based on agricultural waste products (e.g., apple pomace), was optimized. The results showed that the alternative medium contained 15 g apple pomace, 4 g rapeseed meal, 0.1 g KH(2)PO(4), and 0.6 g MgSO(4).7H(2)O in 1 L distilled water. This medium reduced the material costs by 91.5% compared to SSY medium. Response surface methodology (RSM) was used to investigate the influence of environmental variables on production of compounds of antimicrobial metabolites. The optimal conditions achieved were initial pH 7.0, medium volume of 90 mL in 250 mL flasks, rotary speed of 100 rpm, temperature 25 degrees C, and inoculation volume of 15.8%. The antimicrobial activity was increased by 20% by optimizing the environmental parameters. The results obtained allow an efficient production of components with antimicrobial activity by strain Hhs.015(T) on a large scale at low costs.

No MeSH data available.


Related in: MedlinePlus