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In silico identification of the sea squirt selenoproteome.

Jiang L, Liu Q, Ni J - BMC Genomics (2010)

Bottom Line: Among them a selenoprotein W gene was found to have two SECIS elements in the 3'-untranslated region.The method based on SelGenAmic algorithm is capable of identifying eukaryotic selenoprotein genes from their genomes.Application of this method to Ciona intestinalis proves its successes in finding Sec-decoding TGA from large-scale eukaryotic genome sequences, which fills the gap in our knowledge on the ancient chordate selenoproteins.

View Article: PubMed Central - HTML - PubMed

Affiliation: Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, PR China.

ABSTRACT

Background: Computational methods for identifying selenoproteins have been developed rapidly in recent years. However, it is still difficult to identify the open reading frame (ORF) of eukaryotic selenoprotein gene, because the TGA codon for a selenocysteine (Sec) residue in the active centre of selenoprotein is traditionally a terminal signal of protein translation. Although the identification of selenoproteins from genomes through bioinformatics methods has been conducted in bacteria, unicellular eukaryotes, insects and several vertebrates, only a few results have been reported on the ancient chordate selenoproteins.

Results: A gene assembly algorithm SelGenAmic has been constructed and presented in this study for identifying selenoprotein genes from eukaryotic genomes. A method based on this algorithm was developed to build an optimal TGA-containing-ORF for each TGA in a genome, followed by protein similarity analysis through conserved sequence alignments to screen out selenoprotein genes form these ORFs. This method improved the sensitivity of detecting selenoproteins from a genome due to the design that all TGAs in the genome were investigated for its possibility of decoding as a Sec residue. Using this method, eighteen selenoprotein genes were identified from the genome of Ciona intestinalis, leading to its member of selenoproteome up to 19. Among them a selenoprotein W gene was found to have two SECIS elements in the 3'-untranslated region. Additionally, the disulfide bond formation protein A (DsbA) was firstly identified as a selenoprotein in the ancient chordates of Ciona intestinalis, Ciona savignyi and Branchiostoma floridae, while selenoprotein DsbAs had only been found in bacteria and green algae before.

Conclusion: The method based on SelGenAmic algorithm is capable of identifying eukaryotic selenoprotein genes from their genomes. Application of this method to Ciona intestinalis proves its successes in finding Sec-decoding TGA from large-scale eukaryotic genome sequences, which fills the gap in our knowledge on the ancient chordate selenoproteins.

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Gene structure of Ciona intestinalisSelW1. The coding region is indicated by a green rectangle, the untranslated regions by blue rectangles, and the SECIS elements by orange rectangles. The intron is indicated by lines connecting the exons. The position of each site in the sequence of chromosome or scaffold is shown by numbers and the bottom coordinates.
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Figure 6: Gene structure of Ciona intestinalisSelW1. The coding region is indicated by a green rectangle, the untranslated regions by blue rectangles, and the SECIS elements by orange rectangles. The intron is indicated by lines connecting the exons. The position of each site in the sequence of chromosome or scaffold is shown by numbers and the bottom coordinates.

Mentions: Two SECIS elements were detected by SECISearch 2.19 in the 3'-UTR of the SelW1 gene of Ciona intestinalis. The gene structure is shown in Figure 6. All gene structures are schematically shown in this article as the 5'-end on the left. The complete ORF and two SECIS elements are located in the first exon. Two SECIS elements were named SECIS 1 and SECIS 2.


In silico identification of the sea squirt selenoproteome.

Jiang L, Liu Q, Ni J - BMC Genomics (2010)

Gene structure of Ciona intestinalisSelW1. The coding region is indicated by a green rectangle, the untranslated regions by blue rectangles, and the SECIS elements by orange rectangles. The intron is indicated by lines connecting the exons. The position of each site in the sequence of chromosome or scaffold is shown by numbers and the bottom coordinates.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2874816&req=5

Figure 6: Gene structure of Ciona intestinalisSelW1. The coding region is indicated by a green rectangle, the untranslated regions by blue rectangles, and the SECIS elements by orange rectangles. The intron is indicated by lines connecting the exons. The position of each site in the sequence of chromosome or scaffold is shown by numbers and the bottom coordinates.
Mentions: Two SECIS elements were detected by SECISearch 2.19 in the 3'-UTR of the SelW1 gene of Ciona intestinalis. The gene structure is shown in Figure 6. All gene structures are schematically shown in this article as the 5'-end on the left. The complete ORF and two SECIS elements are located in the first exon. Two SECIS elements were named SECIS 1 and SECIS 2.

Bottom Line: Among them a selenoprotein W gene was found to have two SECIS elements in the 3'-untranslated region.The method based on SelGenAmic algorithm is capable of identifying eukaryotic selenoprotein genes from their genomes.Application of this method to Ciona intestinalis proves its successes in finding Sec-decoding TGA from large-scale eukaryotic genome sequences, which fills the gap in our knowledge on the ancient chordate selenoproteins.

View Article: PubMed Central - HTML - PubMed

Affiliation: Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, PR China.

ABSTRACT

Background: Computational methods for identifying selenoproteins have been developed rapidly in recent years. However, it is still difficult to identify the open reading frame (ORF) of eukaryotic selenoprotein gene, because the TGA codon for a selenocysteine (Sec) residue in the active centre of selenoprotein is traditionally a terminal signal of protein translation. Although the identification of selenoproteins from genomes through bioinformatics methods has been conducted in bacteria, unicellular eukaryotes, insects and several vertebrates, only a few results have been reported on the ancient chordate selenoproteins.

Results: A gene assembly algorithm SelGenAmic has been constructed and presented in this study for identifying selenoprotein genes from eukaryotic genomes. A method based on this algorithm was developed to build an optimal TGA-containing-ORF for each TGA in a genome, followed by protein similarity analysis through conserved sequence alignments to screen out selenoprotein genes form these ORFs. This method improved the sensitivity of detecting selenoproteins from a genome due to the design that all TGAs in the genome were investigated for its possibility of decoding as a Sec residue. Using this method, eighteen selenoprotein genes were identified from the genome of Ciona intestinalis, leading to its member of selenoproteome up to 19. Among them a selenoprotein W gene was found to have two SECIS elements in the 3'-untranslated region. Additionally, the disulfide bond formation protein A (DsbA) was firstly identified as a selenoprotein in the ancient chordates of Ciona intestinalis, Ciona savignyi and Branchiostoma floridae, while selenoprotein DsbAs had only been found in bacteria and green algae before.

Conclusion: The method based on SelGenAmic algorithm is capable of identifying eukaryotic selenoprotein genes from their genomes. Application of this method to Ciona intestinalis proves its successes in finding Sec-decoding TGA from large-scale eukaryotic genome sequences, which fills the gap in our knowledge on the ancient chordate selenoproteins.

Show MeSH
Related in: MedlinePlus