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Characterization of microRNA expression in bovine adipose tissues: a potential regulatory mechanism of subcutaneous adipose tissue development.

Jin W, Dodson MV, Moore SS, Basarab JA, Guan LL - BMC Mol. Biol. (2010)

Bottom Line: The miRNA most differentially expressed and the most strongly associated with backfat thickness was miR-378, with a 1.99-fold increase in high backfat thickness tissues (r = 0.72).MiRNA expression patterns differed significantly in response to host genetic components.This result suggests that miRNAs may play a regulatory role in white adipose tissue development in beef animals.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G2P5, Canada.

ABSTRACT

Background: MicroRNAs (miRNAs), a family of small non-coding RNA molecules, appear to regulate animal lipid metabolism and preadipocyte conversion to form lipid-assimilating adipocytes (i.e. adipogenesis). However, no miRNA to date has been reported to modulate adipogenesis and lipid deposition in beef cattle.

Results: The expression patterns of 89 miRNAs including four bovine specific miRNAs in subcutaneous adipose tissues from three groups of crossbred steers differing in backfat thickness were compared using qRT-PCR analysis. Eighty-six miRNAs were detectable in all samples, with 42 miRNAs differing among crossbreds (P < 0.05) and 15 miRNAs differentially expressed between tissues with high and low backfat thickness (P < 0.05). The expression levels of 18 miRNAs were correlated with backfat thickness (P < 0.05). The miRNA most differentially expressed and the most strongly associated with backfat thickness was miR-378, with a 1.99-fold increase in high backfat thickness tissues (r = 0.72).

Conclusions: MiRNA expression patterns differed significantly in response to host genetic components. Approximately 20% of the miRNAs in this study were identified as being correlated with backfat thickness. This result suggests that miRNAs may play a regulatory role in white adipose tissue development in beef animals.

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Related in: MedlinePlus

Expression pattern of miRNAs in back subcutaneous adipose tissues. Heatmap is constructed based on the mean expression levels of miRNAs in each group (n = 4). As shown by the scale bar, increasing green and red signal intensities indicate miRNA with lower or higher expression level, respectively. A1, CHAM with high backfat; A2, CHAM with low backfat; B1, CHAR with high backfat; B2, CHAR with low backfat; C1, HEAN with high backfat; C2, HEAN with low backfat. '#' and '##' indicate that miRNA expression was significant or very significant difference between tissues with high and low backfat thickness, respectively.
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Figure 1: Expression pattern of miRNAs in back subcutaneous adipose tissues. Heatmap is constructed based on the mean expression levels of miRNAs in each group (n = 4). As shown by the scale bar, increasing green and red signal intensities indicate miRNA with lower or higher expression level, respectively. A1, CHAM with high backfat; A2, CHAM with low backfat; B1, CHAR with high backfat; B2, CHAR with low backfat; C1, HEAN with high backfat; C2, HEAN with low backfat. '#' and '##' indicate that miRNA expression was significant or very significant difference between tissues with high and low backfat thickness, respectively.

Mentions: Analysis of the expression of 89 miRNAs including four bovine specific miRNAs (Additional file 1) in beef cattle back subcutaneous adipose tissues confirmed the expression of 86 miRNAs. The animals were divided into 6 groups, according to breed and level of backfat thickness. Hierarchical clustering of the detectable miRNAs showed the expression of miRNAs was more similar in animals of the same breed composition than animals with the same level of backfat thickness (Figure 1). We also compared miRNA expression among three crossbred groups and found the expression of 42 miRNAs to be significantly different among these groups. These results suggest that the expression of miRNAs may differ with regard to host genetic components. CHAR animals have miRNA expression patterns more similar with those of HEAN animals (Figure 1), with 17 significantly different miRNAs. Twenty-nine and 27 miRNAs were found to be significantly different between CHAM and CHAR crossbreds, and between CHAM and HEAN crossbreds, respectively. However, the backfat thickness of CHAR animals was significantly different than that of HEAN animals and was similar with that of CHAM animals (Figure 2).


Characterization of microRNA expression in bovine adipose tissues: a potential regulatory mechanism of subcutaneous adipose tissue development.

Jin W, Dodson MV, Moore SS, Basarab JA, Guan LL - BMC Mol. Biol. (2010)

Expression pattern of miRNAs in back subcutaneous adipose tissues. Heatmap is constructed based on the mean expression levels of miRNAs in each group (n = 4). As shown by the scale bar, increasing green and red signal intensities indicate miRNA with lower or higher expression level, respectively. A1, CHAM with high backfat; A2, CHAM with low backfat; B1, CHAR with high backfat; B2, CHAR with low backfat; C1, HEAN with high backfat; C2, HEAN with low backfat. '#' and '##' indicate that miRNA expression was significant or very significant difference between tissues with high and low backfat thickness, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2874793&req=5

Figure 1: Expression pattern of miRNAs in back subcutaneous adipose tissues. Heatmap is constructed based on the mean expression levels of miRNAs in each group (n = 4). As shown by the scale bar, increasing green and red signal intensities indicate miRNA with lower or higher expression level, respectively. A1, CHAM with high backfat; A2, CHAM with low backfat; B1, CHAR with high backfat; B2, CHAR with low backfat; C1, HEAN with high backfat; C2, HEAN with low backfat. '#' and '##' indicate that miRNA expression was significant or very significant difference between tissues with high and low backfat thickness, respectively.
Mentions: Analysis of the expression of 89 miRNAs including four bovine specific miRNAs (Additional file 1) in beef cattle back subcutaneous adipose tissues confirmed the expression of 86 miRNAs. The animals were divided into 6 groups, according to breed and level of backfat thickness. Hierarchical clustering of the detectable miRNAs showed the expression of miRNAs was more similar in animals of the same breed composition than animals with the same level of backfat thickness (Figure 1). We also compared miRNA expression among three crossbred groups and found the expression of 42 miRNAs to be significantly different among these groups. These results suggest that the expression of miRNAs may differ with regard to host genetic components. CHAR animals have miRNA expression patterns more similar with those of HEAN animals (Figure 1), with 17 significantly different miRNAs. Twenty-nine and 27 miRNAs were found to be significantly different between CHAM and CHAR crossbreds, and between CHAM and HEAN crossbreds, respectively. However, the backfat thickness of CHAR animals was significantly different than that of HEAN animals and was similar with that of CHAM animals (Figure 2).

Bottom Line: The miRNA most differentially expressed and the most strongly associated with backfat thickness was miR-378, with a 1.99-fold increase in high backfat thickness tissues (r = 0.72).MiRNA expression patterns differed significantly in response to host genetic components.This result suggests that miRNAs may play a regulatory role in white adipose tissue development in beef animals.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G2P5, Canada.

ABSTRACT

Background: MicroRNAs (miRNAs), a family of small non-coding RNA molecules, appear to regulate animal lipid metabolism and preadipocyte conversion to form lipid-assimilating adipocytes (i.e. adipogenesis). However, no miRNA to date has been reported to modulate adipogenesis and lipid deposition in beef cattle.

Results: The expression patterns of 89 miRNAs including four bovine specific miRNAs in subcutaneous adipose tissues from three groups of crossbred steers differing in backfat thickness were compared using qRT-PCR analysis. Eighty-six miRNAs were detectable in all samples, with 42 miRNAs differing among crossbreds (P < 0.05) and 15 miRNAs differentially expressed between tissues with high and low backfat thickness (P < 0.05). The expression levels of 18 miRNAs were correlated with backfat thickness (P < 0.05). The miRNA most differentially expressed and the most strongly associated with backfat thickness was miR-378, with a 1.99-fold increase in high backfat thickness tissues (r = 0.72).

Conclusions: MiRNA expression patterns differed significantly in response to host genetic components. Approximately 20% of the miRNAs in this study were identified as being correlated with backfat thickness. This result suggests that miRNAs may play a regulatory role in white adipose tissue development in beef animals.

Show MeSH
Related in: MedlinePlus