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Physiological response to lipid peroxidation in ischemia and reperfusion during carotid endarterectomy.

Banni S, Montisci R, Sanfilippo R, Finco G, Sanna D, Giordano E, Murru E, Cordeddu L, Carta G, Banni D, Marchi A - Lipids Health Dis (2010)

Bottom Line: The results showed that PUFAHP increased significantly after 30 min of reperfusion in patients with controlateral stenosis > 50%, and steeply decreased after 2 hour of reperfusion.Interestingly, IP increased in a similar fashion of PUFAHP but never significantly.Both ratios CD16:2/CLA and DIN/IP also increased significantly after 30 min of reperfusion to decrease thereafter.

View Article: PubMed Central - HTML - PubMed

Affiliation: Università degli Studi di Cagliari - Dipartimento di Biologia Sperimentale - Cittadella, Universitaria - 09042 Monserrato, Cagliari, Italy. banni@unica.it

ABSTRACT

Background: In this study we aimed to assess lipid peroxidation during carotid endarterectomy by the formation of PUFA hydroperoxides (PUFAHP) and isoprostanes (IP) and concomitant peroxisomal beta-oxidation as a physiological mechanism to limit their concentration. Two markers of peroxisomal beta oxidation have been evaluated, formation of 2,3 dinor from IP and conjugated esadecadienoic acid (CD 16:2) from peroxisomal beta-oxidation of conjugated linoleic acid (CLA), an unusual fatty acid present in small concentration in our diet and preferentially beta-oxidised in peroxisomes.The study was conducted on 30 patients undergoing carotid endarterectomy. Blood samplings were performed before, during endarterectomy in the "ischemic phase", and 30 seconds, 30 minutes and 2 hours after reperfusion.

Results: The results showed that PUFAHP increased significantly after 30 min of reperfusion in patients with controlateral stenosis > 50%, and steeply decreased after 2 hour of reperfusion. Interestingly, IP increased in a similar fashion of PUFAHP but never significantly. Both ratios CD16:2/CLA and DIN/IP also increased significantly after 30 min of reperfusion to decrease thereafter.

Conclusions: Our data show that lipid peroxidation takes place only in patients with high controlateral stenosis and within 2 hours occurs a physiological response aimed to decrease IP and PUFAHP by increasing their catabolism in peroxisomes.

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Related in: MedlinePlus

Changes as percentage of the values of ratios between conjugated hexadecadienoic acid (CD 16:2)and its precursor conjugated linoleic acid (CLA) and 2,3 dinor (DIN) and its precursor isoprostanes (IP) as a measure of peroxisomal beta oxidation, in plasma of patients with low controlateral stenosis (A) and high controlateral stenosis (B). Blood samplings were performed before (p1), during endarterectomy in the "ischemic phase" (p2), and 30 seconds (p3) 30 minutes (p4) and 2 hours (p5).
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Figure 3: Changes as percentage of the values of ratios between conjugated hexadecadienoic acid (CD 16:2)and its precursor conjugated linoleic acid (CLA) and 2,3 dinor (DIN) and its precursor isoprostanes (IP) as a measure of peroxisomal beta oxidation, in plasma of patients with low controlateral stenosis (A) and high controlateral stenosis (B). Blood samplings were performed before (p1), during endarterectomy in the "ischemic phase" (p2), and 30 seconds (p3) 30 minutes (p4) and 2 hours (p5).

Mentions: The ratios CD 16:2/CLA and DIN/IP (figure 3) increased significantly in a similar fashion as PUFAHP and IP. Peculiarly, the ratio DIN/IP increased more steeply which seems to prevent a significant increase of IP. In patients with low controlateral stenosis no changes of all parameters measured were detected. All the other plasma fatty acid measured didn't change significantly (data not shown).


Physiological response to lipid peroxidation in ischemia and reperfusion during carotid endarterectomy.

Banni S, Montisci R, Sanfilippo R, Finco G, Sanna D, Giordano E, Murru E, Cordeddu L, Carta G, Banni D, Marchi A - Lipids Health Dis (2010)

Changes as percentage of the values of ratios between conjugated hexadecadienoic acid (CD 16:2)and its precursor conjugated linoleic acid (CLA) and 2,3 dinor (DIN) and its precursor isoprostanes (IP) as a measure of peroxisomal beta oxidation, in plasma of patients with low controlateral stenosis (A) and high controlateral stenosis (B). Blood samplings were performed before (p1), during endarterectomy in the "ischemic phase" (p2), and 30 seconds (p3) 30 minutes (p4) and 2 hours (p5).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2874547&req=5

Figure 3: Changes as percentage of the values of ratios between conjugated hexadecadienoic acid (CD 16:2)and its precursor conjugated linoleic acid (CLA) and 2,3 dinor (DIN) and its precursor isoprostanes (IP) as a measure of peroxisomal beta oxidation, in plasma of patients with low controlateral stenosis (A) and high controlateral stenosis (B). Blood samplings were performed before (p1), during endarterectomy in the "ischemic phase" (p2), and 30 seconds (p3) 30 minutes (p4) and 2 hours (p5).
Mentions: The ratios CD 16:2/CLA and DIN/IP (figure 3) increased significantly in a similar fashion as PUFAHP and IP. Peculiarly, the ratio DIN/IP increased more steeply which seems to prevent a significant increase of IP. In patients with low controlateral stenosis no changes of all parameters measured were detected. All the other plasma fatty acid measured didn't change significantly (data not shown).

Bottom Line: The results showed that PUFAHP increased significantly after 30 min of reperfusion in patients with controlateral stenosis > 50%, and steeply decreased after 2 hour of reperfusion.Interestingly, IP increased in a similar fashion of PUFAHP but never significantly.Both ratios CD16:2/CLA and DIN/IP also increased significantly after 30 min of reperfusion to decrease thereafter.

View Article: PubMed Central - HTML - PubMed

Affiliation: Università degli Studi di Cagliari - Dipartimento di Biologia Sperimentale - Cittadella, Universitaria - 09042 Monserrato, Cagliari, Italy. banni@unica.it

ABSTRACT

Background: In this study we aimed to assess lipid peroxidation during carotid endarterectomy by the formation of PUFA hydroperoxides (PUFAHP) and isoprostanes (IP) and concomitant peroxisomal beta-oxidation as a physiological mechanism to limit their concentration. Two markers of peroxisomal beta oxidation have been evaluated, formation of 2,3 dinor from IP and conjugated esadecadienoic acid (CD 16:2) from peroxisomal beta-oxidation of conjugated linoleic acid (CLA), an unusual fatty acid present in small concentration in our diet and preferentially beta-oxidised in peroxisomes.The study was conducted on 30 patients undergoing carotid endarterectomy. Blood samplings were performed before, during endarterectomy in the "ischemic phase", and 30 seconds, 30 minutes and 2 hours after reperfusion.

Results: The results showed that PUFAHP increased significantly after 30 min of reperfusion in patients with controlateral stenosis > 50%, and steeply decreased after 2 hour of reperfusion. Interestingly, IP increased in a similar fashion of PUFAHP but never significantly. Both ratios CD16:2/CLA and DIN/IP also increased significantly after 30 min of reperfusion to decrease thereafter.

Conclusions: Our data show that lipid peroxidation takes place only in patients with high controlateral stenosis and within 2 hours occurs a physiological response aimed to decrease IP and PUFAHP by increasing their catabolism in peroxisomes.

Show MeSH
Related in: MedlinePlus