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Physiological response to lipid peroxidation in ischemia and reperfusion during carotid endarterectomy.

Banni S, Montisci R, Sanfilippo R, Finco G, Sanna D, Giordano E, Murru E, Cordeddu L, Carta G, Banni D, Marchi A - Lipids Health Dis (2010)

Bottom Line: The results showed that PUFAHP increased significantly after 30 min of reperfusion in patients with controlateral stenosis > 50%, and steeply decreased after 2 hour of reperfusion.Interestingly, IP increased in a similar fashion of PUFAHP but never significantly.Both ratios CD16:2/CLA and DIN/IP also increased significantly after 30 min of reperfusion to decrease thereafter.

View Article: PubMed Central - HTML - PubMed

Affiliation: Università degli Studi di Cagliari - Dipartimento di Biologia Sperimentale - Cittadella, Universitaria - 09042 Monserrato, Cagliari, Italy. banni@unica.it

ABSTRACT

Background: In this study we aimed to assess lipid peroxidation during carotid endarterectomy by the formation of PUFA hydroperoxides (PUFAHP) and isoprostanes (IP) and concomitant peroxisomal beta-oxidation as a physiological mechanism to limit their concentration. Two markers of peroxisomal beta oxidation have been evaluated, formation of 2,3 dinor from IP and conjugated esadecadienoic acid (CD 16:2) from peroxisomal beta-oxidation of conjugated linoleic acid (CLA), an unusual fatty acid present in small concentration in our diet and preferentially beta-oxidised in peroxisomes.The study was conducted on 30 patients undergoing carotid endarterectomy. Blood samplings were performed before, during endarterectomy in the "ischemic phase", and 30 seconds, 30 minutes and 2 hours after reperfusion.

Results: The results showed that PUFAHP increased significantly after 30 min of reperfusion in patients with controlateral stenosis > 50%, and steeply decreased after 2 hour of reperfusion. Interestingly, IP increased in a similar fashion of PUFAHP but never significantly. Both ratios CD16:2/CLA and DIN/IP also increased significantly after 30 min of reperfusion to decrease thereafter.

Conclusions: Our data show that lipid peroxidation takes place only in patients with high controlateral stenosis and within 2 hours occurs a physiological response aimed to decrease IP and PUFAHP by increasing their catabolism in peroxisomes.

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Related in: MedlinePlus

Changes as percentage of the values of polyunsaturated fatty acids hydroperoxides and Isoprostanes (IP) in plasma of patients with low controlateral stenosis (A) and high controlateral stenosis (B). Blood samplings were performed before (p1), during endarterectomy in the "ischemic phase" (p2), and 30 seconds (p3) 30 minutes (p4) and 2 hours (p5). Different letters denote significant differences (p < 0.05).
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Figure 2: Changes as percentage of the values of polyunsaturated fatty acids hydroperoxides and Isoprostanes (IP) in plasma of patients with low controlateral stenosis (A) and high controlateral stenosis (B). Blood samplings were performed before (p1), during endarterectomy in the "ischemic phase" (p2), and 30 seconds (p3) 30 minutes (p4) and 2 hours (p5). Different letters denote significant differences (p < 0.05).

Mentions: As it can be seen from figure 2, in patients with high controlateral stenosis, plasma PUFAHP and IP increased after 30' of reperfusion while decreased thereafter, but only PUFAHP significantly.


Physiological response to lipid peroxidation in ischemia and reperfusion during carotid endarterectomy.

Banni S, Montisci R, Sanfilippo R, Finco G, Sanna D, Giordano E, Murru E, Cordeddu L, Carta G, Banni D, Marchi A - Lipids Health Dis (2010)

Changes as percentage of the values of polyunsaturated fatty acids hydroperoxides and Isoprostanes (IP) in plasma of patients with low controlateral stenosis (A) and high controlateral stenosis (B). Blood samplings were performed before (p1), during endarterectomy in the "ischemic phase" (p2), and 30 seconds (p3) 30 minutes (p4) and 2 hours (p5). Different letters denote significant differences (p < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2874547&req=5

Figure 2: Changes as percentage of the values of polyunsaturated fatty acids hydroperoxides and Isoprostanes (IP) in plasma of patients with low controlateral stenosis (A) and high controlateral stenosis (B). Blood samplings were performed before (p1), during endarterectomy in the "ischemic phase" (p2), and 30 seconds (p3) 30 minutes (p4) and 2 hours (p5). Different letters denote significant differences (p < 0.05).
Mentions: As it can be seen from figure 2, in patients with high controlateral stenosis, plasma PUFAHP and IP increased after 30' of reperfusion while decreased thereafter, but only PUFAHP significantly.

Bottom Line: The results showed that PUFAHP increased significantly after 30 min of reperfusion in patients with controlateral stenosis > 50%, and steeply decreased after 2 hour of reperfusion.Interestingly, IP increased in a similar fashion of PUFAHP but never significantly.Both ratios CD16:2/CLA and DIN/IP also increased significantly after 30 min of reperfusion to decrease thereafter.

View Article: PubMed Central - HTML - PubMed

Affiliation: Università degli Studi di Cagliari - Dipartimento di Biologia Sperimentale - Cittadella, Universitaria - 09042 Monserrato, Cagliari, Italy. banni@unica.it

ABSTRACT

Background: In this study we aimed to assess lipid peroxidation during carotid endarterectomy by the formation of PUFA hydroperoxides (PUFAHP) and isoprostanes (IP) and concomitant peroxisomal beta-oxidation as a physiological mechanism to limit their concentration. Two markers of peroxisomal beta oxidation have been evaluated, formation of 2,3 dinor from IP and conjugated esadecadienoic acid (CD 16:2) from peroxisomal beta-oxidation of conjugated linoleic acid (CLA), an unusual fatty acid present in small concentration in our diet and preferentially beta-oxidised in peroxisomes.The study was conducted on 30 patients undergoing carotid endarterectomy. Blood samplings were performed before, during endarterectomy in the "ischemic phase", and 30 seconds, 30 minutes and 2 hours after reperfusion.

Results: The results showed that PUFAHP increased significantly after 30 min of reperfusion in patients with controlateral stenosis > 50%, and steeply decreased after 2 hour of reperfusion. Interestingly, IP increased in a similar fashion of PUFAHP but never significantly. Both ratios CD16:2/CLA and DIN/IP also increased significantly after 30 min of reperfusion to decrease thereafter.

Conclusions: Our data show that lipid peroxidation takes place only in patients with high controlateral stenosis and within 2 hours occurs a physiological response aimed to decrease IP and PUFAHP by increasing their catabolism in peroxisomes.

Show MeSH
Related in: MedlinePlus