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Physiological response to lipid peroxidation in ischemia and reperfusion during carotid endarterectomy.

Banni S, Montisci R, Sanfilippo R, Finco G, Sanna D, Giordano E, Murru E, Cordeddu L, Carta G, Banni D, Marchi A - Lipids Health Dis (2010)

Bottom Line: The results showed that PUFAHP increased significantly after 30 min of reperfusion in patients with controlateral stenosis > 50%, and steeply decreased after 2 hour of reperfusion.Interestingly, IP increased in a similar fashion of PUFAHP but never significantly.Both ratios CD16:2/CLA and DIN/IP also increased significantly after 30 min of reperfusion to decrease thereafter.

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Affiliation: Università degli Studi di Cagliari - Dipartimento di Biologia Sperimentale - Cittadella, Universitaria - 09042 Monserrato, Cagliari, Italy. banni@unica.it

ABSTRACT

Background: In this study we aimed to assess lipid peroxidation during carotid endarterectomy by the formation of PUFA hydroperoxides (PUFAHP) and isoprostanes (IP) and concomitant peroxisomal beta-oxidation as a physiological mechanism to limit their concentration. Two markers of peroxisomal beta oxidation have been evaluated, formation of 2,3 dinor from IP and conjugated esadecadienoic acid (CD 16:2) from peroxisomal beta-oxidation of conjugated linoleic acid (CLA), an unusual fatty acid present in small concentration in our diet and preferentially beta-oxidised in peroxisomes.The study was conducted on 30 patients undergoing carotid endarterectomy. Blood samplings were performed before, during endarterectomy in the "ischemic phase", and 30 seconds, 30 minutes and 2 hours after reperfusion.

Results: The results showed that PUFAHP increased significantly after 30 min of reperfusion in patients with controlateral stenosis > 50%, and steeply decreased after 2 hour of reperfusion. Interestingly, IP increased in a similar fashion of PUFAHP but never significantly. Both ratios CD16:2/CLA and DIN/IP also increased significantly after 30 min of reperfusion to decrease thereafter.

Conclusions: Our data show that lipid peroxidation takes place only in patients with high controlateral stenosis and within 2 hours occurs a physiological response aimed to decrease IP and PUFAHP by increasing their catabolism in peroxisomes.

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Percentage of controlateral stenosis of the two groups of patients.
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Figure 1: Percentage of controlateral stenosis of the two groups of patients.

Mentions: The 30 surgical patients were divided into two distinct groups according to the degree of stenosis of the internal carotid artery controlateral to the one to be operated. The controlateral carotid was then distinguished into two groups according to the degree of stenosis: up to 40% and greater than 50% (figure 1).


Physiological response to lipid peroxidation in ischemia and reperfusion during carotid endarterectomy.

Banni S, Montisci R, Sanfilippo R, Finco G, Sanna D, Giordano E, Murru E, Cordeddu L, Carta G, Banni D, Marchi A - Lipids Health Dis (2010)

Percentage of controlateral stenosis of the two groups of patients.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2874547&req=5

Figure 1: Percentage of controlateral stenosis of the two groups of patients.
Mentions: The 30 surgical patients were divided into two distinct groups according to the degree of stenosis of the internal carotid artery controlateral to the one to be operated. The controlateral carotid was then distinguished into two groups according to the degree of stenosis: up to 40% and greater than 50% (figure 1).

Bottom Line: The results showed that PUFAHP increased significantly after 30 min of reperfusion in patients with controlateral stenosis > 50%, and steeply decreased after 2 hour of reperfusion.Interestingly, IP increased in a similar fashion of PUFAHP but never significantly.Both ratios CD16:2/CLA and DIN/IP also increased significantly after 30 min of reperfusion to decrease thereafter.

View Article: PubMed Central - HTML - PubMed

Affiliation: Università degli Studi di Cagliari - Dipartimento di Biologia Sperimentale - Cittadella, Universitaria - 09042 Monserrato, Cagliari, Italy. banni@unica.it

ABSTRACT

Background: In this study we aimed to assess lipid peroxidation during carotid endarterectomy by the formation of PUFA hydroperoxides (PUFAHP) and isoprostanes (IP) and concomitant peroxisomal beta-oxidation as a physiological mechanism to limit their concentration. Two markers of peroxisomal beta oxidation have been evaluated, formation of 2,3 dinor from IP and conjugated esadecadienoic acid (CD 16:2) from peroxisomal beta-oxidation of conjugated linoleic acid (CLA), an unusual fatty acid present in small concentration in our diet and preferentially beta-oxidised in peroxisomes.The study was conducted on 30 patients undergoing carotid endarterectomy. Blood samplings were performed before, during endarterectomy in the "ischemic phase", and 30 seconds, 30 minutes and 2 hours after reperfusion.

Results: The results showed that PUFAHP increased significantly after 30 min of reperfusion in patients with controlateral stenosis > 50%, and steeply decreased after 2 hour of reperfusion. Interestingly, IP increased in a similar fashion of PUFAHP but never significantly. Both ratios CD16:2/CLA and DIN/IP also increased significantly after 30 min of reperfusion to decrease thereafter.

Conclusions: Our data show that lipid peroxidation takes place only in patients with high controlateral stenosis and within 2 hours occurs a physiological response aimed to decrease IP and PUFAHP by increasing their catabolism in peroxisomes.

Show MeSH
Related in: MedlinePlus