Limits...
Norovirus gastroenteritis outbreak with a secretor-independent susceptibility pattern, Sweden.

Nordgren J, Kindberg E, Lindgren PE, Matussek A, Svensson L - Emerging Infect. Dis. (2010)

Bottom Line: Susceptibility to disease has been associated with histo-blood group antigens and secretor status; nonsecretors are almost completely resistant to disease.Moreover, no statistical difference in susceptibility was found between persons of different Lewis or ABO phenotypes.This norovirus outbreak affected persons regardless of secretor status or Lewis or ABO phenotypes.

View Article: PubMed Central - PubMed

Affiliation: University of Linkoping, 581 85 Linkoping, Sweden.

ABSTRACT
Norovirus (NoV) is recognized as the commonest cause of acute gastroenteritis among adults. Susceptibility to disease has been associated with histo-blood group antigens and secretor status; nonsecretors are almost completely resistant to disease. We report a foodborne outbreak of GI.3 NoV gastroenteritis that affected 33/83 (40%) persons. Symptomatic disease was as likely to develop in nonsecretors as in secretors (odds ratio [OR] 1.41, 95% confidence interval [CI] 0.46-4.36 vs. OR 0.71, 95% CI 0.23-2.18, p = 0.57). Moreover, no statistical difference in susceptibility was found between persons of different Lewis or ABO phenotypes. The capsid gene of the outbreak strain shares high amino acid homology with the Kashiwa645 GI.3 strain, previously shown to recognize nonsecretor saliva, as well as synthetic Lewis a. This norovirus outbreak affected persons regardless of secretor status or Lewis or ABO phenotypes.

Show MeSH

Related in: MedlinePlus

Phylogenetic analysis of amino acids of the norovirus capsid gene from the gastroenteritis outbreak in Jönköping, Sweden (JKPG, •) and reference strains. The tree was constructed using the neighbor-joining and Poisson correction methods, with MEGA 4.0 software (www.megasoftware.net). Bootstrap values are shown at the branch nodes (values <70% are not shown). Reference sequences were collected from Genbank and represent the 8 genotypes of GI as described by Zheng et al. (26). Scale bar indicates nucleotide substitutions per site. NV-USA [M87661], Aich124-JPN [AB031013], SOV-GBR [L07418], C59-USA [AF435807], HLL219-USA [AF414403], Stav-Nor [AF145709], LR316-USA [AF414405], VA115-USA [AY038598], Kashiwa645-JAP [BD011871], PD196-DEU [AF439267], JKPG-SWE [FJ711163] DSV-USA [U04469], Chiba-JPN [AB042808], Valetta-MLT [AJ277616], Musgrove-GBR [AJ277614], SzUG1-JPN [AB039774], Hesse-DEU [AF093797], Wiscon-USA [AY502008], Wnchest-GBR [AJ277609], Boxer-USA [AF538679].
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2874438&req=5

Figure 2: Phylogenetic analysis of amino acids of the norovirus capsid gene from the gastroenteritis outbreak in Jönköping, Sweden (JKPG, •) and reference strains. The tree was constructed using the neighbor-joining and Poisson correction methods, with MEGA 4.0 software (www.megasoftware.net). Bootstrap values are shown at the branch nodes (values <70% are not shown). Reference sequences were collected from Genbank and represent the 8 genotypes of GI as described by Zheng et al. (26). Scale bar indicates nucleotide substitutions per site. NV-USA [M87661], Aich124-JPN [AB031013], SOV-GBR [L07418], C59-USA [AF435807], HLL219-USA [AF414403], Stav-Nor [AF145709], LR316-USA [AF414405], VA115-USA [AY038598], Kashiwa645-JAP [BD011871], PD196-DEU [AF439267], JKPG-SWE [FJ711163] DSV-USA [U04469], Chiba-JPN [AB042808], Valetta-MLT [AJ277616], Musgrove-GBR [AJ277614], SzUG1-JPN [AB039774], Hesse-DEU [AF093797], Wiscon-USA [AY502008], Wnchest-GBR [AJ277609], Boxer-USA [AF538679].

Mentions: NoV GI was detected by real-time PCR in all collected stool specimens (n = 4); three of these isolates (881–883) were subsequently genotyped by nucleotide sequencing of the N/S region. The fourth sample could not be genotyped because of low virus concentration in the stool sample. Phylogenetic analysis clustered the 3 isolates with NoV GI.3 strains (data not shown). The entire capsid gene was subsequently sequenced from 2 isolates and compared with reference strains (Figure 2). The closest amino acid similarity (98.0%) of the complete capsid gene was found with strain PD196-DEU (GI.3), isolated in Germany 2000, and with the Kashiwa645 (GI.3) strain (97.8%), used in an earlier VLP binding study (14).


Norovirus gastroenteritis outbreak with a secretor-independent susceptibility pattern, Sweden.

Nordgren J, Kindberg E, Lindgren PE, Matussek A, Svensson L - Emerging Infect. Dis. (2010)

Phylogenetic analysis of amino acids of the norovirus capsid gene from the gastroenteritis outbreak in Jönköping, Sweden (JKPG, •) and reference strains. The tree was constructed using the neighbor-joining and Poisson correction methods, with MEGA 4.0 software (www.megasoftware.net). Bootstrap values are shown at the branch nodes (values <70% are not shown). Reference sequences were collected from Genbank and represent the 8 genotypes of GI as described by Zheng et al. (26). Scale bar indicates nucleotide substitutions per site. NV-USA [M87661], Aich124-JPN [AB031013], SOV-GBR [L07418], C59-USA [AF435807], HLL219-USA [AF414403], Stav-Nor [AF145709], LR316-USA [AF414405], VA115-USA [AY038598], Kashiwa645-JAP [BD011871], PD196-DEU [AF439267], JKPG-SWE [FJ711163] DSV-USA [U04469], Chiba-JPN [AB042808], Valetta-MLT [AJ277616], Musgrove-GBR [AJ277614], SzUG1-JPN [AB039774], Hesse-DEU [AF093797], Wiscon-USA [AY502008], Wnchest-GBR [AJ277609], Boxer-USA [AF538679].
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2874438&req=5

Figure 2: Phylogenetic analysis of amino acids of the norovirus capsid gene from the gastroenteritis outbreak in Jönköping, Sweden (JKPG, •) and reference strains. The tree was constructed using the neighbor-joining and Poisson correction methods, with MEGA 4.0 software (www.megasoftware.net). Bootstrap values are shown at the branch nodes (values <70% are not shown). Reference sequences were collected from Genbank and represent the 8 genotypes of GI as described by Zheng et al. (26). Scale bar indicates nucleotide substitutions per site. NV-USA [M87661], Aich124-JPN [AB031013], SOV-GBR [L07418], C59-USA [AF435807], HLL219-USA [AF414403], Stav-Nor [AF145709], LR316-USA [AF414405], VA115-USA [AY038598], Kashiwa645-JAP [BD011871], PD196-DEU [AF439267], JKPG-SWE [FJ711163] DSV-USA [U04469], Chiba-JPN [AB042808], Valetta-MLT [AJ277616], Musgrove-GBR [AJ277614], SzUG1-JPN [AB039774], Hesse-DEU [AF093797], Wiscon-USA [AY502008], Wnchest-GBR [AJ277609], Boxer-USA [AF538679].
Mentions: NoV GI was detected by real-time PCR in all collected stool specimens (n = 4); three of these isolates (881–883) were subsequently genotyped by nucleotide sequencing of the N/S region. The fourth sample could not be genotyped because of low virus concentration in the stool sample. Phylogenetic analysis clustered the 3 isolates with NoV GI.3 strains (data not shown). The entire capsid gene was subsequently sequenced from 2 isolates and compared with reference strains (Figure 2). The closest amino acid similarity (98.0%) of the complete capsid gene was found with strain PD196-DEU (GI.3), isolated in Germany 2000, and with the Kashiwa645 (GI.3) strain (97.8%), used in an earlier VLP binding study (14).

Bottom Line: Susceptibility to disease has been associated with histo-blood group antigens and secretor status; nonsecretors are almost completely resistant to disease.Moreover, no statistical difference in susceptibility was found between persons of different Lewis or ABO phenotypes.This norovirus outbreak affected persons regardless of secretor status or Lewis or ABO phenotypes.

View Article: PubMed Central - PubMed

Affiliation: University of Linkoping, 581 85 Linkoping, Sweden.

ABSTRACT
Norovirus (NoV) is recognized as the commonest cause of acute gastroenteritis among adults. Susceptibility to disease has been associated with histo-blood group antigens and secretor status; nonsecretors are almost completely resistant to disease. We report a foodborne outbreak of GI.3 NoV gastroenteritis that affected 33/83 (40%) persons. Symptomatic disease was as likely to develop in nonsecretors as in secretors (odds ratio [OR] 1.41, 95% confidence interval [CI] 0.46-4.36 vs. OR 0.71, 95% CI 0.23-2.18, p = 0.57). Moreover, no statistical difference in susceptibility was found between persons of different Lewis or ABO phenotypes. The capsid gene of the outbreak strain shares high amino acid homology with the Kashiwa645 GI.3 strain, previously shown to recognize nonsecretor saliva, as well as synthetic Lewis a. This norovirus outbreak affected persons regardless of secretor status or Lewis or ABO phenotypes.

Show MeSH
Related in: MedlinePlus