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Enterotoxigenic Escherichia coli multilocus sequence types in Guatemala and Mexico.

Nicklasson M, Klena J, Rodas C, Bourgeois AL, Torres O, Svennerholm AM, Sjoling A - Emerging Infect. Dis. (2010)

Bottom Line: The genetic backgrounds of 24 enterotoxigenic Escherichia coli (ETEC) strains from Mexico and Guatemala expressing heat-stable toxin (ST) and coli surface antigen 6 (CS6) were analyzed.US travelers to these countries and resident children in Guatemala were infected by ETEC strains of sequence type 398, expressing STp and carrying genetically identical CS6 sequences.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biomedicine, Department of Microbiology and Immunology, University of Gothenburg, Box 435, 405 30 Göteborg, Sweden. matilda.nicklasson@microbio.gu.se

ABSTRACT
The genetic backgrounds of 24 enterotoxigenic Escherichia coli (ETEC) strains from Mexico and Guatemala expressing heat-stable toxin (ST) and coli surface antigen 6 (CS6) were analyzed. US travelers to these countries and resident children in Guatemala were infected by ETEC strains of sequence type 398, expressing STp and carrying genetically identical CS6 sequences.

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Related in: MedlinePlus

Dendrogram of the 24 enterotoxigenic Escherichia coli strains from Guatemala and Mexico included in the study, showing multilocus sequence type. Sequences were assembled with BioEdit and aligned using ClustalX within BioEdit (12). The dendogram represents the relationship of a concatenation of the sequences from each strain and was constructed by using MEGA 3.1 (13). Phylogenetic reconstructions were created by using the neighbor-joining method with the Kimura 2-parameter substitution model, using 1,000 bootstrap replicates. A similar arrangement of the strains was indicated by eBURST version 2 analysis (http://eburst.mlst.net). Scale bar indicates dissimilarity, where 0 is completely identical and 1 is completely dissimilar.
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Figure 1: Dendrogram of the 24 enterotoxigenic Escherichia coli strains from Guatemala and Mexico included in the study, showing multilocus sequence type. Sequences were assembled with BioEdit and aligned using ClustalX within BioEdit (12). The dendogram represents the relationship of a concatenation of the sequences from each strain and was constructed by using MEGA 3.1 (13). Phylogenetic reconstructions were created by using the neighbor-joining method with the Kimura 2-parameter substitution model, using 1,000 bootstrap replicates. A similar arrangement of the strains was indicated by eBURST version 2 analysis (http://eburst.mlst.net). Scale bar indicates dissimilarity, where 0 is completely identical and 1 is completely dissimilar.

Mentions: Seven MLST sequence types were observed among the 24 ST/CS6 ETEC isolates (Table). The most common were MLST sequence type 398 (ST-398) (n = 10 [all from Guatemala]), sequence type 182 (n = 6 [4 from Guatemala, 2 from Mexico]), and sequence type 278 (n = 4 [2 each from Guatemala and Mexico]). Three novel MLST sequence types (all from Guatemala) were identified and, upon submission to the E. coli MLST database, were designated as MLST sequence types 712, 726, and 727. Sequence type 726 clustered closely with the sequence type 182 isolates (Figure); these 2 sequence types are single locus variants, differing only in mdh (mdh-6 v mdh-1). Sequence type 727 is a single locus variant of sequence type 278, differing only in gyrB (gyrB-1 v gyrB-33). Sequence type 712 is a single locus variant of sequence type 398, differing only in fumC (fumC-23 v fumC-7).


Enterotoxigenic Escherichia coli multilocus sequence types in Guatemala and Mexico.

Nicklasson M, Klena J, Rodas C, Bourgeois AL, Torres O, Svennerholm AM, Sjoling A - Emerging Infect. Dis. (2010)

Dendrogram of the 24 enterotoxigenic Escherichia coli strains from Guatemala and Mexico included in the study, showing multilocus sequence type. Sequences were assembled with BioEdit and aligned using ClustalX within BioEdit (12). The dendogram represents the relationship of a concatenation of the sequences from each strain and was constructed by using MEGA 3.1 (13). Phylogenetic reconstructions were created by using the neighbor-joining method with the Kimura 2-parameter substitution model, using 1,000 bootstrap replicates. A similar arrangement of the strains was indicated by eBURST version 2 analysis (http://eburst.mlst.net). Scale bar indicates dissimilarity, where 0 is completely identical and 1 is completely dissimilar.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2874370&req=5

Figure 1: Dendrogram of the 24 enterotoxigenic Escherichia coli strains from Guatemala and Mexico included in the study, showing multilocus sequence type. Sequences were assembled with BioEdit and aligned using ClustalX within BioEdit (12). The dendogram represents the relationship of a concatenation of the sequences from each strain and was constructed by using MEGA 3.1 (13). Phylogenetic reconstructions were created by using the neighbor-joining method with the Kimura 2-parameter substitution model, using 1,000 bootstrap replicates. A similar arrangement of the strains was indicated by eBURST version 2 analysis (http://eburst.mlst.net). Scale bar indicates dissimilarity, where 0 is completely identical and 1 is completely dissimilar.
Mentions: Seven MLST sequence types were observed among the 24 ST/CS6 ETEC isolates (Table). The most common were MLST sequence type 398 (ST-398) (n = 10 [all from Guatemala]), sequence type 182 (n = 6 [4 from Guatemala, 2 from Mexico]), and sequence type 278 (n = 4 [2 each from Guatemala and Mexico]). Three novel MLST sequence types (all from Guatemala) were identified and, upon submission to the E. coli MLST database, were designated as MLST sequence types 712, 726, and 727. Sequence type 726 clustered closely with the sequence type 182 isolates (Figure); these 2 sequence types are single locus variants, differing only in mdh (mdh-6 v mdh-1). Sequence type 727 is a single locus variant of sequence type 278, differing only in gyrB (gyrB-1 v gyrB-33). Sequence type 712 is a single locus variant of sequence type 398, differing only in fumC (fumC-23 v fumC-7).

Bottom Line: The genetic backgrounds of 24 enterotoxigenic Escherichia coli (ETEC) strains from Mexico and Guatemala expressing heat-stable toxin (ST) and coli surface antigen 6 (CS6) were analyzed.US travelers to these countries and resident children in Guatemala were infected by ETEC strains of sequence type 398, expressing STp and carrying genetically identical CS6 sequences.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biomedicine, Department of Microbiology and Immunology, University of Gothenburg, Box 435, 405 30 Göteborg, Sweden. matilda.nicklasson@microbio.gu.se

ABSTRACT
The genetic backgrounds of 24 enterotoxigenic Escherichia coli (ETEC) strains from Mexico and Guatemala expressing heat-stable toxin (ST) and coli surface antigen 6 (CS6) were analyzed. US travelers to these countries and resident children in Guatemala were infected by ETEC strains of sequence type 398, expressing STp and carrying genetically identical CS6 sequences.

Show MeSH
Related in: MedlinePlus