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Increase in plasma concentrations of geranylgeranoic Acid after turmeric tablet intake by healthy volunteers.

Mitake M, Ogawa H, Uebaba K, Shidoji Y - J Clin Biochem Nutr (2010)

Bottom Line: By using liquid chromatography/mass spectrometry, authentic GGA was eluted at a retention time of around 18 min as a negative ion of m/z 303.4.These results indicated that GGA in the turmeric tablet was absorbed as an intact form from intestinal mucosa.The present study provides a clue to conduct a research for cancer preventive roles of GGA in a number of spices.

View Article: PubMed Central - PubMed

Affiliation: Molecular and Cellular Biology, Graduate School of Human Health Sciences, Siebold University of Nagasaki, Nagayo, Nagasaki 851-2195, Japan.

ABSTRACT
Geranylgeranoic acid (GGA) is one of the most potent cancer-preventive acyclic retinoids. GGA has been shown to induce cell death in human hepatoma-derived HuH-7 cells. We have recently reported the natural occurrence of GGA and its related compounds in several medicinal herbs such as turmeric, basil, rosehip, cinnamon and others [Shidoji and Ogawa, J. Lipid Res., 45: 1092-1103, 2004]. In the present study, we performed oral administration of turmeric tablets to healthy volunteers in order to investigate bioavailability of natural GGA. By using liquid chromatography/mass spectrometry, authentic GGA was eluted at a retention time of around 18 min as a negative ion of m/z 303.4. With healthy volunteers, plasma GGA was detected prior to the tablet intake and its concentrations were increased at 2 h after its intake and maintained at higher level until 4 h, suggesting an efficient bioavailability of preformed GGA in the turmeric tablets through oral administration. These results indicated that GGA in the turmeric tablet was absorbed as an intact form from intestinal mucosa. The present study provides a clue to conduct a research for cancer preventive roles of GGA in a number of spices.

No MeSH data available.


Related in: MedlinePlus

LC/MS elution profiles of selected ion chromatograph (negative ions at m/z 303.4) of turmeric tablets and cow milk extracts. A: Authentic GGA and ARA were eluted at the RT of 17.29 min and 13.78 min, respectively. B: A major component in the acidic lipid fraction of the commercial cow milk was eluted at the same RT as that of authentic ARA. C: The 5-fold expanded version (y-axis) of the chromatogram in panel B is shown to demonstrate no detectable GGA in the cow milk extracts. D: The acidic lipid fraction of the turmeric tablet was eluted to show no detectable ARA. E: The 5-fold expanded version (y-axis) of the chromatogram in panel D is to demonstrate a distinct peak of GGA. F: The cochromatogram of standard GGA with the acidic lipid fraction of the turmeric tablet is shown at the same scale as panel E. The down-pointing black arrows indicate the elution position of authentic GGA.
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Figure 1: LC/MS elution profiles of selected ion chromatograph (negative ions at m/z 303.4) of turmeric tablets and cow milk extracts. A: Authentic GGA and ARA were eluted at the RT of 17.29 min and 13.78 min, respectively. B: A major component in the acidic lipid fraction of the commercial cow milk was eluted at the same RT as that of authentic ARA. C: The 5-fold expanded version (y-axis) of the chromatogram in panel B is shown to demonstrate no detectable GGA in the cow milk extracts. D: The acidic lipid fraction of the turmeric tablet was eluted to show no detectable ARA. E: The 5-fold expanded version (y-axis) of the chromatogram in panel D is to demonstrate a distinct peak of GGA. F: The cochromatogram of standard GGA with the acidic lipid fraction of the turmeric tablet is shown at the same scale as panel E. The down-pointing black arrows indicate the elution position of authentic GGA.

Mentions: At the initial place of the present study, we measured GGA contents in commercially available turmeric tablets and cow milk. As shown in Fig. 1A, authentic GGA and ARA were eluted at around 18 min and 14 min, respectively, when mass ions of m/z(−) = 303.4 were traced on LC/MS. Fig. 1B clearly describes that cow milk contained free ARA, a concentration of which was calculated to be 0.3 µg/ml from its peak area. On the other hand, cow milk obtained from a supermarket contained no detectable amount of GGA in our assay system (Fig. 1C) so that cow milk was used as negative control food for the present human study.


Increase in plasma concentrations of geranylgeranoic Acid after turmeric tablet intake by healthy volunteers.

Mitake M, Ogawa H, Uebaba K, Shidoji Y - J Clin Biochem Nutr (2010)

LC/MS elution profiles of selected ion chromatograph (negative ions at m/z 303.4) of turmeric tablets and cow milk extracts. A: Authentic GGA and ARA were eluted at the RT of 17.29 min and 13.78 min, respectively. B: A major component in the acidic lipid fraction of the commercial cow milk was eluted at the same RT as that of authentic ARA. C: The 5-fold expanded version (y-axis) of the chromatogram in panel B is shown to demonstrate no detectable GGA in the cow milk extracts. D: The acidic lipid fraction of the turmeric tablet was eluted to show no detectable ARA. E: The 5-fold expanded version (y-axis) of the chromatogram in panel D is to demonstrate a distinct peak of GGA. F: The cochromatogram of standard GGA with the acidic lipid fraction of the turmeric tablet is shown at the same scale as panel E. The down-pointing black arrows indicate the elution position of authentic GGA.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2872231&req=5

Figure 1: LC/MS elution profiles of selected ion chromatograph (negative ions at m/z 303.4) of turmeric tablets and cow milk extracts. A: Authentic GGA and ARA were eluted at the RT of 17.29 min and 13.78 min, respectively. B: A major component in the acidic lipid fraction of the commercial cow milk was eluted at the same RT as that of authentic ARA. C: The 5-fold expanded version (y-axis) of the chromatogram in panel B is shown to demonstrate no detectable GGA in the cow milk extracts. D: The acidic lipid fraction of the turmeric tablet was eluted to show no detectable ARA. E: The 5-fold expanded version (y-axis) of the chromatogram in panel D is to demonstrate a distinct peak of GGA. F: The cochromatogram of standard GGA with the acidic lipid fraction of the turmeric tablet is shown at the same scale as panel E. The down-pointing black arrows indicate the elution position of authentic GGA.
Mentions: At the initial place of the present study, we measured GGA contents in commercially available turmeric tablets and cow milk. As shown in Fig. 1A, authentic GGA and ARA were eluted at around 18 min and 14 min, respectively, when mass ions of m/z(−) = 303.4 were traced on LC/MS. Fig. 1B clearly describes that cow milk contained free ARA, a concentration of which was calculated to be 0.3 µg/ml from its peak area. On the other hand, cow milk obtained from a supermarket contained no detectable amount of GGA in our assay system (Fig. 1C) so that cow milk was used as negative control food for the present human study.

Bottom Line: By using liquid chromatography/mass spectrometry, authentic GGA was eluted at a retention time of around 18 min as a negative ion of m/z 303.4.These results indicated that GGA in the turmeric tablet was absorbed as an intact form from intestinal mucosa.The present study provides a clue to conduct a research for cancer preventive roles of GGA in a number of spices.

View Article: PubMed Central - PubMed

Affiliation: Molecular and Cellular Biology, Graduate School of Human Health Sciences, Siebold University of Nagasaki, Nagayo, Nagasaki 851-2195, Japan.

ABSTRACT
Geranylgeranoic acid (GGA) is one of the most potent cancer-preventive acyclic retinoids. GGA has been shown to induce cell death in human hepatoma-derived HuH-7 cells. We have recently reported the natural occurrence of GGA and its related compounds in several medicinal herbs such as turmeric, basil, rosehip, cinnamon and others [Shidoji and Ogawa, J. Lipid Res., 45: 1092-1103, 2004]. In the present study, we performed oral administration of turmeric tablets to healthy volunteers in order to investigate bioavailability of natural GGA. By using liquid chromatography/mass spectrometry, authentic GGA was eluted at a retention time of around 18 min as a negative ion of m/z 303.4. With healthy volunteers, plasma GGA was detected prior to the tablet intake and its concentrations were increased at 2 h after its intake and maintained at higher level until 4 h, suggesting an efficient bioavailability of preformed GGA in the turmeric tablets through oral administration. These results indicated that GGA in the turmeric tablet was absorbed as an intact form from intestinal mucosa. The present study provides a clue to conduct a research for cancer preventive roles of GGA in a number of spices.

No MeSH data available.


Related in: MedlinePlus