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The human nasal microbiota and Staphylococcus aureus carriage.

Frank DN, Feazel LM, Bessesen MT, Price CS, Janoff EN, Pace NR - PLoS ONE (2010)

Bottom Line: Moreover, within the inpatient population S. aureus colonization was negatively correlated with the abundances of several microbial groups, including S. epidermidis (p = 0.004).The nares environment is colonized by a temporally stable microbiota that is distinct from other regions of the integument.Negative association between S. aureus, S. epidermidis, and other groups suggests microbial competition during colonization of the nares, a finding that could be exploited to limit S. aureus colonization.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular, Cellular, and Developmental Biology, University of Colorado at Boulder, Boulder, Colorado, United States of America.

ABSTRACT

Background: Colonization of humans with Staphylococcus aureus is a critical prerequisite of subsequent clinical infection of the skin, blood, lung, heart and other deep tissues. S. aureus persistently or intermittently colonizes the nares of approximately 50% of healthy adults, whereas approximately 50% of the general population is rarely or never colonized by this pathogen. Because microbial consortia within the nasal cavity may be an important determinant of S. aureus colonization we determined the composition and dynamics of the nasal microbiota and correlated specific microorganisms with S. aureus colonization.

Methodology/principal findings: Nasal specimens were collected longitudinally from five healthy adults and a cross-section of hospitalized patients (26 S. aureus carriers and 16 non-carriers). Culture-independent analysis of 16S rRNA sequences revealed that the nasal microbiota of healthy subjects consists primarily of members of the phylum Actinobacteria (e.g., Propionibacterium spp. and Corynebacterium spp.), with proportionally less representation of other phyla, including Firmicutes (e.g., Staphylococcus spp.) and Proteobacteria (e.g. Enterobacter spp). In contrast, inpatient nasal microbiotas were enriched in S. aureus or Staphylococcus epidermidis and diminished in several actinobacterial groups, most notably Propionibacterium acnes. Moreover, within the inpatient population S. aureus colonization was negatively correlated with the abundances of several microbial groups, including S. epidermidis (p = 0.004).

Conclusions/significance: The nares environment is colonized by a temporally stable microbiota that is distinct from other regions of the integument. Negative association between S. aureus, S. epidermidis, and other groups suggests microbial competition during colonization of the nares, a finding that could be exploited to limit S. aureus colonization.

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Related in: MedlinePlus

Impact of S. aureus sequences on distributions of other nares bacteria.Panel A. Percent abundance of top twelve genera. Panel B. Percent abundance of top twelve genera adjusted by removal of S. aureus from total sequence counts.
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pone-0010598-g007: Impact of S. aureus sequences on distributions of other nares bacteria.Panel A. Percent abundance of top twelve genera. Panel B. Percent abundance of top twelve genera adjusted by removal of S. aureus from total sequence counts.

Mentions: In a zero-sum assay such as broad-range PCR, a bloom of one type of microbe would lower the proportions of all other microbes in a sequence library, regardless of whether the bloom actually affected the growth of the other microbes. However, removal of S. aureus sequences from the sequence datasets revealed different distributions of microbial groups in S. aureus colonized compared with non-colonized individuals (Fig. 7). Of most relevance, the abundance of S. epidermidis was greatly reduced relative to most other bacterial taxa in S. aureus colonized individuals. This suggests that S. aureus may alter the composition of the underlying nares bacterial communities, rather than simply grow without impacting or displacing other microbial communities.


The human nasal microbiota and Staphylococcus aureus carriage.

Frank DN, Feazel LM, Bessesen MT, Price CS, Janoff EN, Pace NR - PLoS ONE (2010)

Impact of S. aureus sequences on distributions of other nares bacteria.Panel A. Percent abundance of top twelve genera. Panel B. Percent abundance of top twelve genera adjusted by removal of S. aureus from total sequence counts.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2871794&req=5

pone-0010598-g007: Impact of S. aureus sequences on distributions of other nares bacteria.Panel A. Percent abundance of top twelve genera. Panel B. Percent abundance of top twelve genera adjusted by removal of S. aureus from total sequence counts.
Mentions: In a zero-sum assay such as broad-range PCR, a bloom of one type of microbe would lower the proportions of all other microbes in a sequence library, regardless of whether the bloom actually affected the growth of the other microbes. However, removal of S. aureus sequences from the sequence datasets revealed different distributions of microbial groups in S. aureus colonized compared with non-colonized individuals (Fig. 7). Of most relevance, the abundance of S. epidermidis was greatly reduced relative to most other bacterial taxa in S. aureus colonized individuals. This suggests that S. aureus may alter the composition of the underlying nares bacterial communities, rather than simply grow without impacting or displacing other microbial communities.

Bottom Line: Moreover, within the inpatient population S. aureus colonization was negatively correlated with the abundances of several microbial groups, including S. epidermidis (p = 0.004).The nares environment is colonized by a temporally stable microbiota that is distinct from other regions of the integument.Negative association between S. aureus, S. epidermidis, and other groups suggests microbial competition during colonization of the nares, a finding that could be exploited to limit S. aureus colonization.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular, Cellular, and Developmental Biology, University of Colorado at Boulder, Boulder, Colorado, United States of America.

ABSTRACT

Background: Colonization of humans with Staphylococcus aureus is a critical prerequisite of subsequent clinical infection of the skin, blood, lung, heart and other deep tissues. S. aureus persistently or intermittently colonizes the nares of approximately 50% of healthy adults, whereas approximately 50% of the general population is rarely or never colonized by this pathogen. Because microbial consortia within the nasal cavity may be an important determinant of S. aureus colonization we determined the composition and dynamics of the nasal microbiota and correlated specific microorganisms with S. aureus colonization.

Methodology/principal findings: Nasal specimens were collected longitudinally from five healthy adults and a cross-section of hospitalized patients (26 S. aureus carriers and 16 non-carriers). Culture-independent analysis of 16S rRNA sequences revealed that the nasal microbiota of healthy subjects consists primarily of members of the phylum Actinobacteria (e.g., Propionibacterium spp. and Corynebacterium spp.), with proportionally less representation of other phyla, including Firmicutes (e.g., Staphylococcus spp.) and Proteobacteria (e.g. Enterobacter spp). In contrast, inpatient nasal microbiotas were enriched in S. aureus or Staphylococcus epidermidis and diminished in several actinobacterial groups, most notably Propionibacterium acnes. Moreover, within the inpatient population S. aureus colonization was negatively correlated with the abundances of several microbial groups, including S. epidermidis (p = 0.004).

Conclusions/significance: The nares environment is colonized by a temporally stable microbiota that is distinct from other regions of the integument. Negative association between S. aureus, S. epidermidis, and other groups suggests microbial competition during colonization of the nares, a finding that could be exploited to limit S. aureus colonization.

Show MeSH
Related in: MedlinePlus