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The human nasal microbiota and Staphylococcus aureus carriage.

Frank DN, Feazel LM, Bessesen MT, Price CS, Janoff EN, Pace NR - PLoS ONE (2010)

Bottom Line: Moreover, within the inpatient population S. aureus colonization was negatively correlated with the abundances of several microbial groups, including S. epidermidis (p = 0.004).The nares environment is colonized by a temporally stable microbiota that is distinct from other regions of the integument.Negative association between S. aureus, S. epidermidis, and other groups suggests microbial competition during colonization of the nares, a finding that could be exploited to limit S. aureus colonization.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular, Cellular, and Developmental Biology, University of Colorado at Boulder, Boulder, Colorado, United States of America.

ABSTRACT

Background: Colonization of humans with Staphylococcus aureus is a critical prerequisite of subsequent clinical infection of the skin, blood, lung, heart and other deep tissues. S. aureus persistently or intermittently colonizes the nares of approximately 50% of healthy adults, whereas approximately 50% of the general population is rarely or never colonized by this pathogen. Because microbial consortia within the nasal cavity may be an important determinant of S. aureus colonization we determined the composition and dynamics of the nasal microbiota and correlated specific microorganisms with S. aureus colonization.

Methodology/principal findings: Nasal specimens were collected longitudinally from five healthy adults and a cross-section of hospitalized patients (26 S. aureus carriers and 16 non-carriers). Culture-independent analysis of 16S rRNA sequences revealed that the nasal microbiota of healthy subjects consists primarily of members of the phylum Actinobacteria (e.g., Propionibacterium spp. and Corynebacterium spp.), with proportionally less representation of other phyla, including Firmicutes (e.g., Staphylococcus spp.) and Proteobacteria (e.g. Enterobacter spp). In contrast, inpatient nasal microbiotas were enriched in S. aureus or Staphylococcus epidermidis and diminished in several actinobacterial groups, most notably Propionibacterium acnes. Moreover, within the inpatient population S. aureus colonization was negatively correlated with the abundances of several microbial groups, including S. epidermidis (p = 0.004).

Conclusions/significance: The nares environment is colonized by a temporally stable microbiota that is distinct from other regions of the integument. Negative association between S. aureus, S. epidermidis, and other groups suggests microbial competition during colonization of the nares, a finding that could be exploited to limit S. aureus colonization.

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Related in: MedlinePlus

Comparison of community similarity (CMH) between specimen types.The similarity of microbiota in all pairwise combinations of specimens obtained from healthy adults was assessed using the abundance-based Morisita-Horn similarity index (CMH). Boxplots indicate the spread of CMH values calculated for the indicated comparison. Each chart summarizes data for a study participant. For instance, the first boxplot (“A Nar vs. A Nar”) summarizes data for CMH scores for pairs of Subject A nares samples over time. Statistical significances are reported in Table 2.
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pone-0010598-g003: Comparison of community similarity (CMH) between specimen types.The similarity of microbiota in all pairwise combinations of specimens obtained from healthy adults was assessed using the abundance-based Morisita-Horn similarity index (CMH). Boxplots indicate the spread of CMH values calculated for the indicated comparison. Each chart summarizes data for a study participant. For instance, the first boxplot (“A Nar vs. A Nar”) summarizes data for CMH scores for pairs of Subject A nares samples over time. Statistical significances are reported in Table 2.

Mentions: Quantitative assessment of similarities between populations (using the Morisita-Horn similarity index) indicated that the nares, axilla and groin harbor distinct and temporally stable microbial communities (Figs. 2 and 3, Table 2). In general, nares samples were more similar to one another than to groin or axilla specimens, even when collected from the same host on the same day (e.g., p<0.001 for intra-subject comparisons of axilla vs. groin, axilla vs. nares, and groin vs. nares in Subject A; Table 2). Interestingly, the axilla and groin microbiotas of Subject A were no more similar to the nares communities of Subject A than to those of Subjects B–E (Fig. 2 and 3, Table 2).


The human nasal microbiota and Staphylococcus aureus carriage.

Frank DN, Feazel LM, Bessesen MT, Price CS, Janoff EN, Pace NR - PLoS ONE (2010)

Comparison of community similarity (CMH) between specimen types.The similarity of microbiota in all pairwise combinations of specimens obtained from healthy adults was assessed using the abundance-based Morisita-Horn similarity index (CMH). Boxplots indicate the spread of CMH values calculated for the indicated comparison. Each chart summarizes data for a study participant. For instance, the first boxplot (“A Nar vs. A Nar”) summarizes data for CMH scores for pairs of Subject A nares samples over time. Statistical significances are reported in Table 2.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2871794&req=5

pone-0010598-g003: Comparison of community similarity (CMH) between specimen types.The similarity of microbiota in all pairwise combinations of specimens obtained from healthy adults was assessed using the abundance-based Morisita-Horn similarity index (CMH). Boxplots indicate the spread of CMH values calculated for the indicated comparison. Each chart summarizes data for a study participant. For instance, the first boxplot (“A Nar vs. A Nar”) summarizes data for CMH scores for pairs of Subject A nares samples over time. Statistical significances are reported in Table 2.
Mentions: Quantitative assessment of similarities between populations (using the Morisita-Horn similarity index) indicated that the nares, axilla and groin harbor distinct and temporally stable microbial communities (Figs. 2 and 3, Table 2). In general, nares samples were more similar to one another than to groin or axilla specimens, even when collected from the same host on the same day (e.g., p<0.001 for intra-subject comparisons of axilla vs. groin, axilla vs. nares, and groin vs. nares in Subject A; Table 2). Interestingly, the axilla and groin microbiotas of Subject A were no more similar to the nares communities of Subject A than to those of Subjects B–E (Fig. 2 and 3, Table 2).

Bottom Line: Moreover, within the inpatient population S. aureus colonization was negatively correlated with the abundances of several microbial groups, including S. epidermidis (p = 0.004).The nares environment is colonized by a temporally stable microbiota that is distinct from other regions of the integument.Negative association between S. aureus, S. epidermidis, and other groups suggests microbial competition during colonization of the nares, a finding that could be exploited to limit S. aureus colonization.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular, Cellular, and Developmental Biology, University of Colorado at Boulder, Boulder, Colorado, United States of America.

ABSTRACT

Background: Colonization of humans with Staphylococcus aureus is a critical prerequisite of subsequent clinical infection of the skin, blood, lung, heart and other deep tissues. S. aureus persistently or intermittently colonizes the nares of approximately 50% of healthy adults, whereas approximately 50% of the general population is rarely or never colonized by this pathogen. Because microbial consortia within the nasal cavity may be an important determinant of S. aureus colonization we determined the composition and dynamics of the nasal microbiota and correlated specific microorganisms with S. aureus colonization.

Methodology/principal findings: Nasal specimens were collected longitudinally from five healthy adults and a cross-section of hospitalized patients (26 S. aureus carriers and 16 non-carriers). Culture-independent analysis of 16S rRNA sequences revealed that the nasal microbiota of healthy subjects consists primarily of members of the phylum Actinobacteria (e.g., Propionibacterium spp. and Corynebacterium spp.), with proportionally less representation of other phyla, including Firmicutes (e.g., Staphylococcus spp.) and Proteobacteria (e.g. Enterobacter spp). In contrast, inpatient nasal microbiotas were enriched in S. aureus or Staphylococcus epidermidis and diminished in several actinobacterial groups, most notably Propionibacterium acnes. Moreover, within the inpatient population S. aureus colonization was negatively correlated with the abundances of several microbial groups, including S. epidermidis (p = 0.004).

Conclusions/significance: The nares environment is colonized by a temporally stable microbiota that is distinct from other regions of the integument. Negative association between S. aureus, S. epidermidis, and other groups suggests microbial competition during colonization of the nares, a finding that could be exploited to limit S. aureus colonization.

Show MeSH
Related in: MedlinePlus