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Dielectrophoresis: a review of applications for stem cell research.

Pethig R, Menachery A, Pells S, De Sousa P - J. Biomed. Biotechnol. (2010)

Bottom Line: Demonstrated capabilities include the enrichment of haematopoetic stem cells from bone marrow and peripheral blood, and adult stem cells from adipose tissue.Recent research suggests that this technique can predict the ultimate fate of neural stem cells after differentiation before the appearance of specific cell-surface proteins.This review summarises the properties of cells that contribute to their dielectrophoretic behaviour, and their relevance to stem cell research and translational applications.

View Article: PubMed Central - PubMed

Affiliation: Institute for Integrated Micro and Nano Systems, Joint Research Institute for Integrated Systems, School of Engineering, The University of Edinburgh, Edinburgh EH9 3JF, UK. ron.pethig@ed.ac.uk

ABSTRACT
Dielectrophoresis can discriminate distinct cellular identities in heterogeneous populations, and monitor cell state changes associated with activation and clonal expansion, apoptosis, and necrosis, without the need for biochemical labels. Demonstrated capabilities include the enrichment of haematopoetic stem cells from bone marrow and peripheral blood, and adult stem cells from adipose tissue. Recent research suggests that this technique can predict the ultimate fate of neural stem cells after differentiation before the appearance of specific cell-surface proteins. This review summarises the properties of cells that contribute to their dielectrophoretic behaviour, and their relevance to stem cell research and translational applications.

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Related in: MedlinePlus

DEP cell trapping efficiency curves for embryonic mouse neural stem/precursor cells (NSPC), neurons and astrocytes, based on the results reported by Flanagan et al. [7]. Extrapolating this (positive) DEP data to zero cell trapping indicates that these three different cell types exhibit different fxo1 cross-over frequencies. Such differences should enable the selective isolation of these different cell types from each other using DEP (e.g., [1–3]).
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fig5: DEP cell trapping efficiency curves for embryonic mouse neural stem/precursor cells (NSPC), neurons and astrocytes, based on the results reported by Flanagan et al. [7]. Extrapolating this (positive) DEP data to zero cell trapping indicates that these three different cell types exhibit different fxo1 cross-over frequencies. Such differences should enable the selective isolation of these different cell types from each other using DEP (e.g., [1–3]).

Mentions: In the Flanagan et al. study, DEP signatures for the neural stem cells were obtained by monitoring the rate at which they collected at the electrodes [7]. Figure 5 highlights the important aspects of these results, to show that the three cell types investigated exhibited markedly different DEP signatures. This can also be deduced by extrapolating for values of the DEP cross-over frequency fxo1 for the cell types. In their DEP experiments Flanagan et al. [7] did not change the conductivity of the cell suspending electrolyte, and cell viability was checked at all times. From (4) we can deduce that the two cell properties responsible for the different characteristics shown in Figure 5 are cell size and membrane capacitance (possibly reflecting differences in cell surface topography [4, 6]). However, cell size was not determined (or at least reported) by Flanagan et al. [7], and so we are unable to relate the observed DEP differences to intrinsic properties related to surface properties of the cell membrane. Commitment of stem cells to different lineages is regulated by many cues, including cell shape. For example, it has been demonstrated that human mesenchymal stem cells (hMSCs) allowed to adhere, flatten, and spread out underwent osteogenesis, while unspread, round cells underwent adipogenesis [23]. The shape of hMSCs is also involved in the decision between chondrogenic or smooth muscle cell fates in response to TGFβ3 signalling [24]. It will be important to the DEP characterisation of stem cells to discover to what extent different cell shapes during cell culture translate to different cell surface complexities (e.g., extent of membrane folding or ruffles), and hence different measurable DEP properties after they have rounded up when suspended in DEP measurement media.


Dielectrophoresis: a review of applications for stem cell research.

Pethig R, Menachery A, Pells S, De Sousa P - J. Biomed. Biotechnol. (2010)

DEP cell trapping efficiency curves for embryonic mouse neural stem/precursor cells (NSPC), neurons and astrocytes, based on the results reported by Flanagan et al. [7]. Extrapolating this (positive) DEP data to zero cell trapping indicates that these three different cell types exhibit different fxo1 cross-over frequencies. Such differences should enable the selective isolation of these different cell types from each other using DEP (e.g., [1–3]).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2871555&req=5

fig5: DEP cell trapping efficiency curves for embryonic mouse neural stem/precursor cells (NSPC), neurons and astrocytes, based on the results reported by Flanagan et al. [7]. Extrapolating this (positive) DEP data to zero cell trapping indicates that these three different cell types exhibit different fxo1 cross-over frequencies. Such differences should enable the selective isolation of these different cell types from each other using DEP (e.g., [1–3]).
Mentions: In the Flanagan et al. study, DEP signatures for the neural stem cells were obtained by monitoring the rate at which they collected at the electrodes [7]. Figure 5 highlights the important aspects of these results, to show that the three cell types investigated exhibited markedly different DEP signatures. This can also be deduced by extrapolating for values of the DEP cross-over frequency fxo1 for the cell types. In their DEP experiments Flanagan et al. [7] did not change the conductivity of the cell suspending electrolyte, and cell viability was checked at all times. From (4) we can deduce that the two cell properties responsible for the different characteristics shown in Figure 5 are cell size and membrane capacitance (possibly reflecting differences in cell surface topography [4, 6]). However, cell size was not determined (or at least reported) by Flanagan et al. [7], and so we are unable to relate the observed DEP differences to intrinsic properties related to surface properties of the cell membrane. Commitment of stem cells to different lineages is regulated by many cues, including cell shape. For example, it has been demonstrated that human mesenchymal stem cells (hMSCs) allowed to adhere, flatten, and spread out underwent osteogenesis, while unspread, round cells underwent adipogenesis [23]. The shape of hMSCs is also involved in the decision between chondrogenic or smooth muscle cell fates in response to TGFβ3 signalling [24]. It will be important to the DEP characterisation of stem cells to discover to what extent different cell shapes during cell culture translate to different cell surface complexities (e.g., extent of membrane folding or ruffles), and hence different measurable DEP properties after they have rounded up when suspended in DEP measurement media.

Bottom Line: Demonstrated capabilities include the enrichment of haematopoetic stem cells from bone marrow and peripheral blood, and adult stem cells from adipose tissue.Recent research suggests that this technique can predict the ultimate fate of neural stem cells after differentiation before the appearance of specific cell-surface proteins.This review summarises the properties of cells that contribute to their dielectrophoretic behaviour, and their relevance to stem cell research and translational applications.

View Article: PubMed Central - PubMed

Affiliation: Institute for Integrated Micro and Nano Systems, Joint Research Institute for Integrated Systems, School of Engineering, The University of Edinburgh, Edinburgh EH9 3JF, UK. ron.pethig@ed.ac.uk

ABSTRACT
Dielectrophoresis can discriminate distinct cellular identities in heterogeneous populations, and monitor cell state changes associated with activation and clonal expansion, apoptosis, and necrosis, without the need for biochemical labels. Demonstrated capabilities include the enrichment of haematopoetic stem cells from bone marrow and peripheral blood, and adult stem cells from adipose tissue. Recent research suggests that this technique can predict the ultimate fate of neural stem cells after differentiation before the appearance of specific cell-surface proteins. This review summarises the properties of cells that contribute to their dielectrophoretic behaviour, and their relevance to stem cell research and translational applications.

Show MeSH
Related in: MedlinePlus