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SnoPatrol: how many snoRNA genes are there?

Gardner PP, Bateman A, Poole AM - J. Biol. (2010)

Bottom Line: Small nucleolar RNAs (snoRNAs) are among the most evolutionarily ancient classes of small RNA.Two experimental screens published in BMC Genomics expand the eukaryotic snoRNA catalog, but many more snoRNAs remain to be found.

View Article: PubMed Central - HTML - PubMed

Affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB101SA, UK. pg5@sanger.ac.uk

ABSTRACT
Small nucleolar RNAs (snoRNAs) are among the most evolutionarily ancient classes of small RNA. Two experimental screens published in BMC Genomics expand the eukaryotic snoRNA catalog, but many more snoRNAs remain to be found.

Show MeSH
snoRNA structure. The structure of a H/ACA snoRNA (left) and a C/D box snoRNA (right). The targets for RNA modification are shown in blue. The most important snoRNA-associated proteins are listed below.
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Figure 1: snoRNA structure. The structure of a H/ACA snoRNA (left) and a C/D box snoRNA (right). The targets for RNA modification are shown in blue. The most important snoRNA-associated proteins are listed below.

Mentions: The biosynthesis of eukaryote ribosomes is complex, involving numerous processing events to generate mature ribosomal RNAs (rRNAs) and the subsequent assembly of processed rRNAs with dozens of ribosomal proteins. Small nucleolar RNAs (snoRNAs) are central to ribosome maturation, being required in key cleavage steps to generate individual rRNAs, and in their capacity as guides for site-specific modification of rRNA. In the rRNA of the budding yeast Saccharomyces cerevisiae, on the order of 100 snoRNA-guided modifications are made during the biosynthesis of a single ribosome; this number is approximately double in humans. Around half of these modifications are methylations of the 2' position on ribose, and are carried out by C/D-box small nucleolar ribonucleoproteins (snoRNPs), which consist of a guide snoRNA acting in concert with several proteins, including Nop1p, the RNA methylase component of the snoRNP. The remaining modifications produce pseudouridine, an isomer of uridine, and are guided by H/ACA-box snoRNPs, with the Cbf5p subunit performing the pseudouridylation reaction [1]. Figure 1 illustrates the interaction between the two types of snoRNA and their respective RNA targets.


SnoPatrol: how many snoRNA genes are there?

Gardner PP, Bateman A, Poole AM - J. Biol. (2010)

snoRNA structure. The structure of a H/ACA snoRNA (left) and a C/D box snoRNA (right). The targets for RNA modification are shown in blue. The most important snoRNA-associated proteins are listed below.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2871523&req=5

Figure 1: snoRNA structure. The structure of a H/ACA snoRNA (left) and a C/D box snoRNA (right). The targets for RNA modification are shown in blue. The most important snoRNA-associated proteins are listed below.
Mentions: The biosynthesis of eukaryote ribosomes is complex, involving numerous processing events to generate mature ribosomal RNAs (rRNAs) and the subsequent assembly of processed rRNAs with dozens of ribosomal proteins. Small nucleolar RNAs (snoRNAs) are central to ribosome maturation, being required in key cleavage steps to generate individual rRNAs, and in their capacity as guides for site-specific modification of rRNA. In the rRNA of the budding yeast Saccharomyces cerevisiae, on the order of 100 snoRNA-guided modifications are made during the biosynthesis of a single ribosome; this number is approximately double in humans. Around half of these modifications are methylations of the 2' position on ribose, and are carried out by C/D-box small nucleolar ribonucleoproteins (snoRNPs), which consist of a guide snoRNA acting in concert with several proteins, including Nop1p, the RNA methylase component of the snoRNP. The remaining modifications produce pseudouridine, an isomer of uridine, and are guided by H/ACA-box snoRNPs, with the Cbf5p subunit performing the pseudouridylation reaction [1]. Figure 1 illustrates the interaction between the two types of snoRNA and their respective RNA targets.

Bottom Line: Small nucleolar RNAs (snoRNAs) are among the most evolutionarily ancient classes of small RNA.Two experimental screens published in BMC Genomics expand the eukaryotic snoRNA catalog, but many more snoRNAs remain to be found.

View Article: PubMed Central - HTML - PubMed

Affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB101SA, UK. pg5@sanger.ac.uk

ABSTRACT
Small nucleolar RNAs (snoRNAs) are among the most evolutionarily ancient classes of small RNA. Two experimental screens published in BMC Genomics expand the eukaryotic snoRNA catalog, but many more snoRNAs remain to be found.

Show MeSH