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Mic1-3 Knockout Toxoplasma gondii is a good candidate for a vaccine against T. gondii-induced abortion in sheep.

Mévélec MN, Ducournau C, Bassuny Ismael A, Olivier M, Sèche E, Lebrun M, Bout D, Dimier-Poisson I - Vet. Res. (2010)

Bottom Line: Both subcutaneous and intraperitoneal injections were effective.Moreover, preliminary results showed the potential of Mic1-3KO to reduce the development of tissue cysts in lambs born to vaccinated ewes.This study demonstrates that Mic1-3KO is a potent vaccine candidate.

View Article: PubMed Central - PubMed

Affiliation: Université François Rabelais, INRA, UMR 0483 Université-INRA d'Immunologie Parasitaire, Vaccinologie et Biothérapie Anti-infectieuse, IFR136 Agents Transmissibles et Infectiologie, UFR de Pharmacie, 31 avenue Monge, 37200 Tours, France. mevelec@univ-tours.fr

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Related in: MedlinePlus

Experiment 1 and Experiment 2: Exp. 1, two groups of Romanov ewes received single vaccinations s.c. by single injection with either 105 Mic1-3KO tachyzoites or 105 S48 tachyzoites. Exp. 2, two groups of Bizet ewes were vaccinated s.c. by single injection with either 105 or 2 × 106 Mic1-3KO tachyzoites. One group in each experiment was not vaccinated (control). All ewes were challenged per-os with 400 sporulated oocysts of the PRU strain at mid gestation. (A) Mean rectal ± S.D. temperature of groups and (B) systemic IgG against T. gondii measured by ELISA after vaccination (mean and S.D.), line indicates positive cut off point. (C) Mean ± S.D. rectal temperature following challenge.
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Figure 1: Experiment 1 and Experiment 2: Exp. 1, two groups of Romanov ewes received single vaccinations s.c. by single injection with either 105 Mic1-3KO tachyzoites or 105 S48 tachyzoites. Exp. 2, two groups of Bizet ewes were vaccinated s.c. by single injection with either 105 or 2 × 106 Mic1-3KO tachyzoites. One group in each experiment was not vaccinated (control). All ewes were challenged per-os with 400 sporulated oocysts of the PRU strain at mid gestation. (A) Mean rectal ± S.D. temperature of groups and (B) systemic IgG against T. gondii measured by ELISA after vaccination (mean and S.D.), line indicates positive cut off point. (C) Mean ± S.D. rectal temperature following challenge.


Mic1-3 Knockout Toxoplasma gondii is a good candidate for a vaccine against T. gondii-induced abortion in sheep.

Mévélec MN, Ducournau C, Bassuny Ismael A, Olivier M, Sèche E, Lebrun M, Bout D, Dimier-Poisson I - Vet. Res. (2010)

Experiment 1 and Experiment 2: Exp. 1, two groups of Romanov ewes received single vaccinations s.c. by single injection with either 105 Mic1-3KO tachyzoites or 105 S48 tachyzoites. Exp. 2, two groups of Bizet ewes were vaccinated s.c. by single injection with either 105 or 2 × 106 Mic1-3KO tachyzoites. One group in each experiment was not vaccinated (control). All ewes were challenged per-os with 400 sporulated oocysts of the PRU strain at mid gestation. (A) Mean rectal ± S.D. temperature of groups and (B) systemic IgG against T. gondii measured by ELISA after vaccination (mean and S.D.), line indicates positive cut off point. (C) Mean ± S.D. rectal temperature following challenge.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2865876&req=5

Figure 1: Experiment 1 and Experiment 2: Exp. 1, two groups of Romanov ewes received single vaccinations s.c. by single injection with either 105 Mic1-3KO tachyzoites or 105 S48 tachyzoites. Exp. 2, two groups of Bizet ewes were vaccinated s.c. by single injection with either 105 or 2 × 106 Mic1-3KO tachyzoites. One group in each experiment was not vaccinated (control). All ewes were challenged per-os with 400 sporulated oocysts of the PRU strain at mid gestation. (A) Mean rectal ± S.D. temperature of groups and (B) systemic IgG against T. gondii measured by ELISA after vaccination (mean and S.D.), line indicates positive cut off point. (C) Mean ± S.D. rectal temperature following challenge.
Bottom Line: Both subcutaneous and intraperitoneal injections were effective.Moreover, preliminary results showed the potential of Mic1-3KO to reduce the development of tissue cysts in lambs born to vaccinated ewes.This study demonstrates that Mic1-3KO is a potent vaccine candidate.

View Article: PubMed Central - PubMed

Affiliation: Université François Rabelais, INRA, UMR 0483 Université-INRA d'Immunologie Parasitaire, Vaccinologie et Biothérapie Anti-infectieuse, IFR136 Agents Transmissibles et Infectiologie, UFR de Pharmacie, 31 avenue Monge, 37200 Tours, France. mevelec@univ-tours.fr

Show MeSH
Related in: MedlinePlus