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Pathogenesis of natural goat scrapie: modulation by host PRNP genotype and effect of co-existent conditions.

González L, Martin S, Hawkins SA, Goldmann W, Jeffrey M, Sisó S - Vet. Res. (2010)

Bottom Line: Unexpectedly, accumulation of disease-associated PrP (PrPd) in the brain was more frequent in methionine carriers at codon 142 (24/32, 75.0%) than amongst isoleucine homozygotes (14/40, 35.0%).The latter, however, showed significantly greater amounts of brain PrPd than the former (average scores of 9.3 and 3.0, respectively).These results, together with the early and consistent involvement of the circumventricular organs and the hypothalamus, point towards a significant contribution of the haematogenous route in the process of neuroinvasion.

View Article: PubMed Central - PubMed

Affiliation: Veterinary Laboratories Agency (VLA-Lasswade), Pentlands Science Park, Penicuik, Midlothian EH26 0PZ, United Kingdom. l.gonzalez@vla.defra.gsi.gov.uk

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Accumulation of PrPd in CVO and related brain structures. a, d, g: Gross sections showing location of the AP at the level of the obex (a), of the SFO at the level of the anterior hypothalamus (d), and of the ME at the level of the infundibular hypothalamus (g). b, e, h: Low power micrographs corresponding to the framed areas of a, d and g, respectively. PrPd is seen to accumulate in the framed areas that correspond to the AP (b), SFO (e) and ME (h); IHC with Bar224 PrP antibody and haematoxylin counterstaining (b, ×2; e, ×1; h, ×1). c, f, i: Detail of PrPd accumulating in the same CVO as in b, e and h, respectively; IHC with Bar224 PrP antibody and haematoxylin counterstaining (c, ×10; f, ×4; i, ×4). (For a color version of this figure, please consult www.vetres.org.)
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Figure 4: Accumulation of PrPd in CVO and related brain structures. a, d, g: Gross sections showing location of the AP at the level of the obex (a), of the SFO at the level of the anterior hypothalamus (d), and of the ME at the level of the infundibular hypothalamus (g). b, e, h: Low power micrographs corresponding to the framed areas of a, d and g, respectively. PrPd is seen to accumulate in the framed areas that correspond to the AP (b), SFO (e) and ME (h); IHC with Bar224 PrP antibody and haematoxylin counterstaining (b, ×2; e, ×1; h, ×1). c, f, i: Detail of PrPd accumulating in the same CVO as in b, e and h, respectively; IHC with Bar224 PrP antibody and haematoxylin counterstaining (c, ×10; f, ×4; i, ×4). (For a color version of this figure, please consult www.vetres.org.)


Pathogenesis of natural goat scrapie: modulation by host PRNP genotype and effect of co-existent conditions.

González L, Martin S, Hawkins SA, Goldmann W, Jeffrey M, Sisó S - Vet. Res. (2010)

Accumulation of PrPd in CVO and related brain structures. a, d, g: Gross sections showing location of the AP at the level of the obex (a), of the SFO at the level of the anterior hypothalamus (d), and of the ME at the level of the infundibular hypothalamus (g). b, e, h: Low power micrographs corresponding to the framed areas of a, d and g, respectively. PrPd is seen to accumulate in the framed areas that correspond to the AP (b), SFO (e) and ME (h); IHC with Bar224 PrP antibody and haematoxylin counterstaining (b, ×2; e, ×1; h, ×1). c, f, i: Detail of PrPd accumulating in the same CVO as in b, e and h, respectively; IHC with Bar224 PrP antibody and haematoxylin counterstaining (c, ×10; f, ×4; i, ×4). (For a color version of this figure, please consult www.vetres.org.)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2865875&req=5

Figure 4: Accumulation of PrPd in CVO and related brain structures. a, d, g: Gross sections showing location of the AP at the level of the obex (a), of the SFO at the level of the anterior hypothalamus (d), and of the ME at the level of the infundibular hypothalamus (g). b, e, h: Low power micrographs corresponding to the framed areas of a, d and g, respectively. PrPd is seen to accumulate in the framed areas that correspond to the AP (b), SFO (e) and ME (h); IHC with Bar224 PrP antibody and haematoxylin counterstaining (b, ×2; e, ×1; h, ×1). c, f, i: Detail of PrPd accumulating in the same CVO as in b, e and h, respectively; IHC with Bar224 PrP antibody and haematoxylin counterstaining (c, ×10; f, ×4; i, ×4). (For a color version of this figure, please consult www.vetres.org.)
Bottom Line: Unexpectedly, accumulation of disease-associated PrP (PrPd) in the brain was more frequent in methionine carriers at codon 142 (24/32, 75.0%) than amongst isoleucine homozygotes (14/40, 35.0%).The latter, however, showed significantly greater amounts of brain PrPd than the former (average scores of 9.3 and 3.0, respectively).These results, together with the early and consistent involvement of the circumventricular organs and the hypothalamus, point towards a significant contribution of the haematogenous route in the process of neuroinvasion.

View Article: PubMed Central - PubMed

Affiliation: Veterinary Laboratories Agency (VLA-Lasswade), Pentlands Science Park, Penicuik, Midlothian EH26 0PZ, United Kingdom. l.gonzalez@vla.defra.gsi.gov.uk

Show MeSH
Related in: MedlinePlus