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Pathogenesis of natural goat scrapie: modulation by host PRNP genotype and effect of co-existent conditions.

González L, Martin S, Hawkins SA, Goldmann W, Jeffrey M, Sisó S - Vet. Res. (2010)

Bottom Line: Unexpectedly, accumulation of disease-associated PrP (PrPd) in the brain was more frequent in methionine carriers at codon 142 (24/32, 75.0%) than amongst isoleucine homozygotes (14/40, 35.0%).The latter, however, showed significantly greater amounts of brain PrPd than the former (average scores of 9.3 and 3.0, respectively).These results, together with the early and consistent involvement of the circumventricular organs and the hypothalamus, point towards a significant contribution of the haematogenous route in the process of neuroinvasion.

View Article: PubMed Central - PubMed

Affiliation: Veterinary Laboratories Agency (VLA-Lasswade), Pentlands Science Park, Penicuik, Midlothian EH26 0PZ, United Kingdom. l.gonzalez@vla.defra.gsi.gov.uk

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(a) Trace PrPd deposits in a single ganglion cell of the myenteric plexus of a goat that was positive in 7 of 10 LRS tissues and also in brain (score 1.7). (b) Widespread PrPd accumulation in ganglion and satellite cells of the ENS of a clinically-affected goat (9/10 LRS positive tissues and 20.0 score in brain). Mild PrPd deposition in the intermedio-lateral column (c) and substantia gelationosa (d) of the TSC of a goat that was positive in 7 of 10 LRS tissues and in brain (score 7.0). IHC with Barr224 PrP antibody and haematoxylin counterstaining (magnifications: a, d ×20; b ×40; c ×10). (For a color version of this figure, please consult www.vetres.org.)
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Figure 2: (a) Trace PrPd deposits in a single ganglion cell of the myenteric plexus of a goat that was positive in 7 of 10 LRS tissues and also in brain (score 1.7). (b) Widespread PrPd accumulation in ganglion and satellite cells of the ENS of a clinically-affected goat (9/10 LRS positive tissues and 20.0 score in brain). Mild PrPd deposition in the intermedio-lateral column (c) and substantia gelationosa (d) of the TSC of a goat that was positive in 7 of 10 LRS tissues and in brain (score 7.0). IHC with Barr224 PrP antibody and haematoxylin counterstaining (magnifications: a, d ×20; b ×40; c ×10). (For a color version of this figure, please consult www.vetres.org.)


Pathogenesis of natural goat scrapie: modulation by host PRNP genotype and effect of co-existent conditions.

González L, Martin S, Hawkins SA, Goldmann W, Jeffrey M, Sisó S - Vet. Res. (2010)

(a) Trace PrPd deposits in a single ganglion cell of the myenteric plexus of a goat that was positive in 7 of 10 LRS tissues and also in brain (score 1.7). (b) Widespread PrPd accumulation in ganglion and satellite cells of the ENS of a clinically-affected goat (9/10 LRS positive tissues and 20.0 score in brain). Mild PrPd deposition in the intermedio-lateral column (c) and substantia gelationosa (d) of the TSC of a goat that was positive in 7 of 10 LRS tissues and in brain (score 7.0). IHC with Barr224 PrP antibody and haematoxylin counterstaining (magnifications: a, d ×20; b ×40; c ×10). (For a color version of this figure, please consult www.vetres.org.)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2865875&req=5

Figure 2: (a) Trace PrPd deposits in a single ganglion cell of the myenteric plexus of a goat that was positive in 7 of 10 LRS tissues and also in brain (score 1.7). (b) Widespread PrPd accumulation in ganglion and satellite cells of the ENS of a clinically-affected goat (9/10 LRS positive tissues and 20.0 score in brain). Mild PrPd deposition in the intermedio-lateral column (c) and substantia gelationosa (d) of the TSC of a goat that was positive in 7 of 10 LRS tissues and in brain (score 7.0). IHC with Barr224 PrP antibody and haematoxylin counterstaining (magnifications: a, d ×20; b ×40; c ×10). (For a color version of this figure, please consult www.vetres.org.)
Bottom Line: Unexpectedly, accumulation of disease-associated PrP (PrPd) in the brain was more frequent in methionine carriers at codon 142 (24/32, 75.0%) than amongst isoleucine homozygotes (14/40, 35.0%).The latter, however, showed significantly greater amounts of brain PrPd than the former (average scores of 9.3 and 3.0, respectively).These results, together with the early and consistent involvement of the circumventricular organs and the hypothalamus, point towards a significant contribution of the haematogenous route in the process of neuroinvasion.

View Article: PubMed Central - PubMed

Affiliation: Veterinary Laboratories Agency (VLA-Lasswade), Pentlands Science Park, Penicuik, Midlothian EH26 0PZ, United Kingdom. l.gonzalez@vla.defra.gsi.gov.uk

Show MeSH
Related in: MedlinePlus