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Interferon-gamma, interleukin-4 and interleukin-10 production by T helper cells reveals intact Th1 and regulatory TR1 cell activation and a delay of the Th2 cell response in equine neonates and foals.

Wagner B, Burton A, Ainsworth D - Vet. Res. (2010)

Bottom Line: In agreement with previous reports, all three cytokines were quantitatively reduced in foals compared to adults.Overall, IL-4 production was low in foals.Relative percentages of IL-4+ Th2 cells were significantly lower in foals at all time points.

View Article: PubMed Central - PubMed

Affiliation: Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. bw73@cornell.edu

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Flow cytometric analysis of CD4+ and CD8+ IFN-γ producing lymphocytes. PBMC from foals of different age groups and from adult horses were stimulated with PMA and ionomycin. The cells were stained for intracellular IFN-γ and cell surface CD4 or CD8 expression. (A) Flow cytometric analysis of one representative horse per age group. (B) Relative percentages of CD4+/IFN-γ+ and (C) CD8+/IFN-γ+ T cells in foals and adult horses within the IFN-γ producing cells (= 100%). (D) Percentages of IFN-γ+/IL-10+ lymphocytes in foals and adult horses. (E) A tri-color staining was performed on stimulated PBMC to confirm that the majority of the IFN-γ+/IL-10+ cells are CD4+ T cells. Gates were set on lymphocytes (see Fig. 1A) and on IFN-γ+ cells (left panel). The IFN-γ+ lymphocytes were then analyzed for IL-10 and CD4 staining (right panel). The horizontal lines within the data sets B, C and D represent medians. *** p < 0.001; ** p = 0.001 to < 0.01; ns = not significant.
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Figure 4: Flow cytometric analysis of CD4+ and CD8+ IFN-γ producing lymphocytes. PBMC from foals of different age groups and from adult horses were stimulated with PMA and ionomycin. The cells were stained for intracellular IFN-γ and cell surface CD4 or CD8 expression. (A) Flow cytometric analysis of one representative horse per age group. (B) Relative percentages of CD4+/IFN-γ+ and (C) CD8+/IFN-γ+ T cells in foals and adult horses within the IFN-γ producing cells (= 100%). (D) Percentages of IFN-γ+/IL-10+ lymphocytes in foals and adult horses. (E) A tri-color staining was performed on stimulated PBMC to confirm that the majority of the IFN-γ+/IL-10+ cells are CD4+ T cells. Gates were set on lymphocytes (see Fig. 1A) and on IFN-γ+ cells (left panel). The IFN-γ+ lymphocytes were then analyzed for IL-10 and CD4 staining (right panel). The horizontal lines within the data sets B, C and D represent medians. *** p < 0.001; ** p = 0.001 to < 0.01; ns = not significant.


Interferon-gamma, interleukin-4 and interleukin-10 production by T helper cells reveals intact Th1 and regulatory TR1 cell activation and a delay of the Th2 cell response in equine neonates and foals.

Wagner B, Burton A, Ainsworth D - Vet. Res. (2010)

Flow cytometric analysis of CD4+ and CD8+ IFN-γ producing lymphocytes. PBMC from foals of different age groups and from adult horses were stimulated with PMA and ionomycin. The cells were stained for intracellular IFN-γ and cell surface CD4 or CD8 expression. (A) Flow cytometric analysis of one representative horse per age group. (B) Relative percentages of CD4+/IFN-γ+ and (C) CD8+/IFN-γ+ T cells in foals and adult horses within the IFN-γ producing cells (= 100%). (D) Percentages of IFN-γ+/IL-10+ lymphocytes in foals and adult horses. (E) A tri-color staining was performed on stimulated PBMC to confirm that the majority of the IFN-γ+/IL-10+ cells are CD4+ T cells. Gates were set on lymphocytes (see Fig. 1A) and on IFN-γ+ cells (left panel). The IFN-γ+ lymphocytes were then analyzed for IL-10 and CD4 staining (right panel). The horizontal lines within the data sets B, C and D represent medians. *** p < 0.001; ** p = 0.001 to < 0.01; ns = not significant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
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getmorefigures.php?uid=PMC2865874&req=5

Figure 4: Flow cytometric analysis of CD4+ and CD8+ IFN-γ producing lymphocytes. PBMC from foals of different age groups and from adult horses were stimulated with PMA and ionomycin. The cells were stained for intracellular IFN-γ and cell surface CD4 or CD8 expression. (A) Flow cytometric analysis of one representative horse per age group. (B) Relative percentages of CD4+/IFN-γ+ and (C) CD8+/IFN-γ+ T cells in foals and adult horses within the IFN-γ producing cells (= 100%). (D) Percentages of IFN-γ+/IL-10+ lymphocytes in foals and adult horses. (E) A tri-color staining was performed on stimulated PBMC to confirm that the majority of the IFN-γ+/IL-10+ cells are CD4+ T cells. Gates were set on lymphocytes (see Fig. 1A) and on IFN-γ+ cells (left panel). The IFN-γ+ lymphocytes were then analyzed for IL-10 and CD4 staining (right panel). The horizontal lines within the data sets B, C and D represent medians. *** p < 0.001; ** p = 0.001 to < 0.01; ns = not significant.
Bottom Line: In agreement with previous reports, all three cytokines were quantitatively reduced in foals compared to adults.Overall, IL-4 production was low in foals.Relative percentages of IL-4+ Th2 cells were significantly lower in foals at all time points.

View Article: PubMed Central - PubMed

Affiliation: Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. bw73@cornell.edu

Show MeSH