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Spectrophotometric and chromatographic simultaneous estimation of amitriptyline hydrochloride and chlordiazepoxide in tablet dosage forms.

Patel S, Patel NJ - Indian J Pharm Sci (2009)

Bottom Line: The separation was carried out on Merck thin layer chromatographic aluminium sheets of silica gel 60 F254 using carbon tetrachloride: acetone: triethylamine (6:3:0.2, v/v/v) as mobile phase.The linearity was found to be in the range of 50-600 and 20-240 ng/spot for amitriptyline hydrochloride and chlordiazepoxide, respectively.The methods were successively applied to pharmaceutical formulation because no chromatographic interferences from the tablet excipients were found.

View Article: PubMed Central - PubMed

Affiliation: S. K. Patel College of Pharmaceutical Education and Research, Department of Pharmaceutical Chemistry, Ganpat University, Kherva, Mehsana-382711, Gujarat, India.

ABSTRACT
A binary mixture of amitriptyline HCl and chlordiazepoxide was determined by three different methods. The first method involved determination of amitriptyline HCl and chlordiazepoxide using the first derivative spectrophotometric technique at 219 and 230 nm over the concentration ranges of 1-20 and 2-24 mug/ml with mean accuracies 100.9+/-0.87 and 99.2+/-1.0%, respectively. The second method was reversed-phase high performance liquid chromatography using methanol: acetonitrile: 0.065 M ammonium acetate buffer (50:20:30, v/v/v), final pH adjust to 5.5 +/- 0.02 with ortho phosphoric acid as the mobile phase and was pumped at a flow rate of 1.0 ml/min. Quantification was achieved with ultraviolet detection at 240 nm over concentration ranges of 0.25-4 and 0.1-1.6 mug/ml; mean accuracies were 100.55+/-0.62 and 100.71+/-0.81%, respectively. The third method utilized high performance thin layer chromatography method in tablet dosage form. The method was based on separation of the two drugs followed by densitometric measurements of their spots at 240 nm. The separation was carried out on Merck thin layer chromatographic aluminium sheets of silica gel 60 F254 using carbon tetrachloride: acetone: triethylamine (6:3:0.2, v/v/v) as mobile phase. The linearity was found to be in the range of 50-600 and 20-240 ng/spot for amitriptyline hydrochloride and chlordiazepoxide, respectively. The methods were successively applied to pharmaceutical formulation because no chromatographic interferences from the tablet excipients were found. The suitability of these methods for the quantitative determination of the compounds was proved by validation.

No MeSH data available.


Related in: MedlinePlus

HPTLC Chromatogram of AMI and CLR at 240 nm. AMI is amitriptyline HCl and CLR is chlordiazepoxide
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Figure 0003: HPTLC Chromatogram of AMI and CLR at 240 nm. AMI is amitriptyline HCl and CLR is chlordiazepoxide

Mentions: To optimize the HPTLC parameters, several mobile phase compositions were tried. Carbon tetrachloride: acetone: triethyleamine (6:3:0.2, v/v/v), gave a sharp and symmetrical peaks of AMI and CLR with Rf values of 0.68±0.02 and 0.29±0.01, respectively (fig. 3). Well-defined spots (and peaks) were obtained when the chamber was saturated with mobile phase vapour for 30 min at room temperature (25°). A linear relation was obtained between peak area and the concentration of the two drugs in the range of 50-600 ng/spot and 20-240 ng/spot for AMI and CLR, respectively. The linear regression Eqns were, Y=8.0515X+37.333, r= 0.9996 and Y= 13.771X+88.616, r= 0.9952, where Y is the area under the peak, X is the concentration in μg/ml, and r is the correlation coefficient.


Spectrophotometric and chromatographic simultaneous estimation of amitriptyline hydrochloride and chlordiazepoxide in tablet dosage forms.

Patel S, Patel NJ - Indian J Pharm Sci (2009)

HPTLC Chromatogram of AMI and CLR at 240 nm. AMI is amitriptyline HCl and CLR is chlordiazepoxide
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2865828&req=5

Figure 0003: HPTLC Chromatogram of AMI and CLR at 240 nm. AMI is amitriptyline HCl and CLR is chlordiazepoxide
Mentions: To optimize the HPTLC parameters, several mobile phase compositions were tried. Carbon tetrachloride: acetone: triethyleamine (6:3:0.2, v/v/v), gave a sharp and symmetrical peaks of AMI and CLR with Rf values of 0.68±0.02 and 0.29±0.01, respectively (fig. 3). Well-defined spots (and peaks) were obtained when the chamber was saturated with mobile phase vapour for 30 min at room temperature (25°). A linear relation was obtained between peak area and the concentration of the two drugs in the range of 50-600 ng/spot and 20-240 ng/spot for AMI and CLR, respectively. The linear regression Eqns were, Y=8.0515X+37.333, r= 0.9996 and Y= 13.771X+88.616, r= 0.9952, where Y is the area under the peak, X is the concentration in μg/ml, and r is the correlation coefficient.

Bottom Line: The separation was carried out on Merck thin layer chromatographic aluminium sheets of silica gel 60 F254 using carbon tetrachloride: acetone: triethylamine (6:3:0.2, v/v/v) as mobile phase.The linearity was found to be in the range of 50-600 and 20-240 ng/spot for amitriptyline hydrochloride and chlordiazepoxide, respectively.The methods were successively applied to pharmaceutical formulation because no chromatographic interferences from the tablet excipients were found.

View Article: PubMed Central - PubMed

Affiliation: S. K. Patel College of Pharmaceutical Education and Research, Department of Pharmaceutical Chemistry, Ganpat University, Kherva, Mehsana-382711, Gujarat, India.

ABSTRACT
A binary mixture of amitriptyline HCl and chlordiazepoxide was determined by three different methods. The first method involved determination of amitriptyline HCl and chlordiazepoxide using the first derivative spectrophotometric technique at 219 and 230 nm over the concentration ranges of 1-20 and 2-24 mug/ml with mean accuracies 100.9+/-0.87 and 99.2+/-1.0%, respectively. The second method was reversed-phase high performance liquid chromatography using methanol: acetonitrile: 0.065 M ammonium acetate buffer (50:20:30, v/v/v), final pH adjust to 5.5 +/- 0.02 with ortho phosphoric acid as the mobile phase and was pumped at a flow rate of 1.0 ml/min. Quantification was achieved with ultraviolet detection at 240 nm over concentration ranges of 0.25-4 and 0.1-1.6 mug/ml; mean accuracies were 100.55+/-0.62 and 100.71+/-0.81%, respectively. The third method utilized high performance thin layer chromatography method in tablet dosage form. The method was based on separation of the two drugs followed by densitometric measurements of their spots at 240 nm. The separation was carried out on Merck thin layer chromatographic aluminium sheets of silica gel 60 F254 using carbon tetrachloride: acetone: triethylamine (6:3:0.2, v/v/v) as mobile phase. The linearity was found to be in the range of 50-600 and 20-240 ng/spot for amitriptyline hydrochloride and chlordiazepoxide, respectively. The methods were successively applied to pharmaceutical formulation because no chromatographic interferences from the tablet excipients were found. The suitability of these methods for the quantitative determination of the compounds was proved by validation.

No MeSH data available.


Related in: MedlinePlus