Limits...
Development and Validation of HPTLC Method for the Estimation of Clotrimazole in Bulk drug and Tablet Formulation.

Parmar P, Mehta A - Indian J Pharm Sci (2009)

Bottom Line: The mobile phase used was a mixture of cyclohexane:toluene:methanol:triethyleamine (8:2:0.5:0.2 v/v/v/v).The method was validated in terms of linearity, accuracy, precision and specificity.The proposed method can be successfully used to determine the drug content of bulk drug and marketed formulation of tablet.

View Article: PubMed Central - PubMed

Affiliation: L. M. College of Pharmacy, Navrangpura, Gujarat-380 009, India.

ABSTRACT
A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the determination of clotrimazole in bulk drug and tablet dosage form. The stationary phase used was precoated silica gel 60F(254). The mobile phase used was a mixture of cyclohexane:toluene:methanol:triethyleamine (8:2:0.5:0.2 v/v/v/v). The detection of spot was carried out at 262 nm. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 200 to 1000 ng/spot for clotrimazole. The limit of detection and the limit of quantification for clotrimazole were found to be 50 ng/spot and 200 ng/spot, respectively. The proposed method can be successfully used to determine the drug content of bulk drug and marketed formulation of tablet.

No MeSH data available.


Related in: MedlinePlus

Limit of detection of clotrimazolePeaks 1 to 6 represent concentration range from 25 ng/spot to 150 ng/spot
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Figure 0003: Limit of detection of clotrimazolePeaks 1 to 6 represent concentration range from 25 ng/spot to 150 ng/spot

Mentions: The limit of detection is the lowest amount of analyte in a sample that can be detected but not necessarily quantitated under the stated experimental conditions. Clotrimazole standard solutions (5 μg/ml) in the quantities of 5, 10, 15, 20, 25 and 30 μl were spotted on TLC plate, developed, dried in hot air and photometrically analyzed as described. The limit of detection was found 50 ng/spot (fig. 3). The limit of quantification (LOQ) is the lowest concentration of analyte in a sample that can be determined with the acceptable precision and accuracy under stated experimental conditions. Lowest concentration of calibration curve for standard clotrimazole was considered as LOQ. The LOQ was found 200 ng/spot (fig. 4).


Development and Validation of HPTLC Method for the Estimation of Clotrimazole in Bulk drug and Tablet Formulation.

Parmar P, Mehta A - Indian J Pharm Sci (2009)

Limit of detection of clotrimazolePeaks 1 to 6 represent concentration range from 25 ng/spot to 150 ng/spot
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2865822&req=5

Figure 0003: Limit of detection of clotrimazolePeaks 1 to 6 represent concentration range from 25 ng/spot to 150 ng/spot
Mentions: The limit of detection is the lowest amount of analyte in a sample that can be detected but not necessarily quantitated under the stated experimental conditions. Clotrimazole standard solutions (5 μg/ml) in the quantities of 5, 10, 15, 20, 25 and 30 μl were spotted on TLC plate, developed, dried in hot air and photometrically analyzed as described. The limit of detection was found 50 ng/spot (fig. 3). The limit of quantification (LOQ) is the lowest concentration of analyte in a sample that can be determined with the acceptable precision and accuracy under stated experimental conditions. Lowest concentration of calibration curve for standard clotrimazole was considered as LOQ. The LOQ was found 200 ng/spot (fig. 4).

Bottom Line: The mobile phase used was a mixture of cyclohexane:toluene:methanol:triethyleamine (8:2:0.5:0.2 v/v/v/v).The method was validated in terms of linearity, accuracy, precision and specificity.The proposed method can be successfully used to determine the drug content of bulk drug and marketed formulation of tablet.

View Article: PubMed Central - PubMed

Affiliation: L. M. College of Pharmacy, Navrangpura, Gujarat-380 009, India.

ABSTRACT
A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the determination of clotrimazole in bulk drug and tablet dosage form. The stationary phase used was precoated silica gel 60F(254). The mobile phase used was a mixture of cyclohexane:toluene:methanol:triethyleamine (8:2:0.5:0.2 v/v/v/v). The detection of spot was carried out at 262 nm. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 200 to 1000 ng/spot for clotrimazole. The limit of detection and the limit of quantification for clotrimazole were found to be 50 ng/spot and 200 ng/spot, respectively. The proposed method can be successfully used to determine the drug content of bulk drug and marketed formulation of tablet.

No MeSH data available.


Related in: MedlinePlus