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Study of degradation profile and development of stability indicating methods for cefixime trihydrate.

Gandhi SP, Rajput SJ - Indian J Pharm Sci (2009)

Bottom Line: The UV spectral characteristics of the drug and degraded products were quite different and zero and first order derivative ultraviolet spectrophotometric methods were used to study the extent of degradation.Cefixime trihydrate was found to degrade extensively under experimental conditions.The methods were validated by establishing the linearity, inter and intraday precision, accuracy, selectivity and specificity.

View Article: PubMed Central - PubMed

Affiliation: Pharmaceutical Quality Assurance Laboratory, Pharmacy Department, Faculty of Technology & Engineering, M. S. University of Baroda, Vadodara-390 001, India.

ABSTRACT
The degradation behavior of cefixime trihydrate was investigated under different stress conditions of acidic hydrolysis, alkaline hydrolysis and oxidation using spectrophotometry. Stability indicating spectrophotometric methods were developed that could separate the drug from its degradation products formed under these stress conditions. The UV spectral characteristics of the drug and degraded products were quite different and zero and first order derivative ultraviolet spectrophotometric methods were used to study the extent of degradation. Cefixime trihydrate was found to degrade extensively under experimental conditions. The methods were validated by establishing the linearity, inter and intraday precision, accuracy, selectivity and specificity.

No MeSH data available.


Overlapped first derivative spectra of cefixime and its acid degraded solutionCefixime was estimated at 242 nm, the zero crossing point for acid degraded solution.
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Figure 0002: Overlapped first derivative spectra of cefixime and its acid degraded solutionCefixime was estimated at 242 nm, the zero crossing point for acid degraded solution.

Mentions: The zero order UV spectrum of CEF showed maximum absorbance at 287 nm. The solutions which were totally degraded by NaOH and H2O2 did not show any absorbance at this wavelength so decrease in absorbance was a direct measure of extent of degradation in partially degraded solutions (fig. 1). Though the spectral characteristics of acid degraded solution were quite different from the standard drug, the solution showed substantial absorption at 287 nm. The overlapped first order derivative curve (fig. 2) for both CEF and HCl degraded solution were obtained and zero crossing points for CEF and acid degraded solution were determined. CEF showed zero absorbance at 225 nm so this wavelength was used to estimate acid degraded solution and CEF was estimated at 242 nm at which the absorbance was zero for acid treated solution.


Study of degradation profile and development of stability indicating methods for cefixime trihydrate.

Gandhi SP, Rajput SJ - Indian J Pharm Sci (2009)

Overlapped first derivative spectra of cefixime and its acid degraded solutionCefixime was estimated at 242 nm, the zero crossing point for acid degraded solution.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2865818&req=5

Figure 0002: Overlapped first derivative spectra of cefixime and its acid degraded solutionCefixime was estimated at 242 nm, the zero crossing point for acid degraded solution.
Mentions: The zero order UV spectrum of CEF showed maximum absorbance at 287 nm. The solutions which were totally degraded by NaOH and H2O2 did not show any absorbance at this wavelength so decrease in absorbance was a direct measure of extent of degradation in partially degraded solutions (fig. 1). Though the spectral characteristics of acid degraded solution were quite different from the standard drug, the solution showed substantial absorption at 287 nm. The overlapped first order derivative curve (fig. 2) for both CEF and HCl degraded solution were obtained and zero crossing points for CEF and acid degraded solution were determined. CEF showed zero absorbance at 225 nm so this wavelength was used to estimate acid degraded solution and CEF was estimated at 242 nm at which the absorbance was zero for acid treated solution.

Bottom Line: The UV spectral characteristics of the drug and degraded products were quite different and zero and first order derivative ultraviolet spectrophotometric methods were used to study the extent of degradation.Cefixime trihydrate was found to degrade extensively under experimental conditions.The methods were validated by establishing the linearity, inter and intraday precision, accuracy, selectivity and specificity.

View Article: PubMed Central - PubMed

Affiliation: Pharmaceutical Quality Assurance Laboratory, Pharmacy Department, Faculty of Technology & Engineering, M. S. University of Baroda, Vadodara-390 001, India.

ABSTRACT
The degradation behavior of cefixime trihydrate was investigated under different stress conditions of acidic hydrolysis, alkaline hydrolysis and oxidation using spectrophotometry. Stability indicating spectrophotometric methods were developed that could separate the drug from its degradation products formed under these stress conditions. The UV spectral characteristics of the drug and degraded products were quite different and zero and first order derivative ultraviolet spectrophotometric methods were used to study the extent of degradation. Cefixime trihydrate was found to degrade extensively under experimental conditions. The methods were validated by establishing the linearity, inter and intraday precision, accuracy, selectivity and specificity.

No MeSH data available.