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Cefoperazone sodium impregnated polycaprolactone composite implant for osteomyelitis.

Anand A, Pundir R, Pandian CS, Saraf S, Gupta H - Indian J Pharm Sci (2009)

Bottom Line: The pellet were also tested for microbiological efficacy and compared with plain drug solution in different concentrations.The release profile displayed drug levels above MIC continuously up to 2 months.Wide zone of inhibition by pellet against Staph. aureus as compared to drug solution proves its efficacy in treatment of osteomyelitis.

View Article: PubMed Central - PubMed

Affiliation: Babu Banarasi Das National Institute of Technology & Management, Lucknow-226 007, India.

ABSTRACT
The use of local antibiotics from a biodegradable implant for chronic osteomyelitis is an attractive alternative. The implant delivers high antibiotic concentration at tissue levels, obliterates dead space, aids bone repair and does not need to be removed. The purpose of this paper is to develop and evaluate a calcium sulphate and polycaprolactone based composite biodegradable implantable delivery system of cefoperazone sodium. Implants were prepared by modified fabrication technique to avoid solvent use. Interaction studies were carried out to check any incompatibility between ingredients. Prepared implants were evaluated for various in vitro parameters like dimensions, hardness, tensile strength, drug release profile and sterility. Morphological changes in pellet before and after drug release were evaluated by scanning electron microscopy. The pellet were also tested for microbiological efficacy and compared with plain drug solution in different concentrations. Developed pellets are regular in shape and size with good tensile strength. The release profile displayed drug levels above MIC continuously up to 2 months. Wide zone of inhibition by pellet against Staph. aureus as compared to drug solution proves its efficacy in treatment of osteomyelitis.

No MeSH data available.


Related in: MedlinePlus

Zone of inhibition in cup plate assay.(a) Different drug concentration (1) Blank Solution (2) 15 μg/ml concentration (3) 30 μg/ml concentration (b) Pellet formulation
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Figure 0004: Zone of inhibition in cup plate assay.(a) Different drug concentration (1) Blank Solution (2) 15 μg/ml concentration (3) 30 μg/ml concentration (b) Pellet formulation

Mentions: Cup plate assay of the plain cefoperazone sodium solution showed inhibitory activity of this drug against the S. aureus. Zone of inhibition is concentration dependent as clearly seen, Table 1. The cup carrying 30 μg/ml (fig. 4a, 3) concentration shows a bigger zone of inhibition as compared to 15 μg/ml concentration (fig. 4a, 2). Diffusion of drug from the pellets also showed a clear zone of inhibition. A large zone of inhibition (almost covering entire plate) was observed at 24 h in plate carrying the intact pellet, fig. 4b. It indicates efficacy of developed pellets in comparison to simple drug solution.


Cefoperazone sodium impregnated polycaprolactone composite implant for osteomyelitis.

Anand A, Pundir R, Pandian CS, Saraf S, Gupta H - Indian J Pharm Sci (2009)

Zone of inhibition in cup plate assay.(a) Different drug concentration (1) Blank Solution (2) 15 μg/ml concentration (3) 30 μg/ml concentration (b) Pellet formulation
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2865808&req=5

Figure 0004: Zone of inhibition in cup plate assay.(a) Different drug concentration (1) Blank Solution (2) 15 μg/ml concentration (3) 30 μg/ml concentration (b) Pellet formulation
Mentions: Cup plate assay of the plain cefoperazone sodium solution showed inhibitory activity of this drug against the S. aureus. Zone of inhibition is concentration dependent as clearly seen, Table 1. The cup carrying 30 μg/ml (fig. 4a, 3) concentration shows a bigger zone of inhibition as compared to 15 μg/ml concentration (fig. 4a, 2). Diffusion of drug from the pellets also showed a clear zone of inhibition. A large zone of inhibition (almost covering entire plate) was observed at 24 h in plate carrying the intact pellet, fig. 4b. It indicates efficacy of developed pellets in comparison to simple drug solution.

Bottom Line: The pellet were also tested for microbiological efficacy and compared with plain drug solution in different concentrations.The release profile displayed drug levels above MIC continuously up to 2 months.Wide zone of inhibition by pellet against Staph. aureus as compared to drug solution proves its efficacy in treatment of osteomyelitis.

View Article: PubMed Central - PubMed

Affiliation: Babu Banarasi Das National Institute of Technology & Management, Lucknow-226 007, India.

ABSTRACT
The use of local antibiotics from a biodegradable implant for chronic osteomyelitis is an attractive alternative. The implant delivers high antibiotic concentration at tissue levels, obliterates dead space, aids bone repair and does not need to be removed. The purpose of this paper is to develop and evaluate a calcium sulphate and polycaprolactone based composite biodegradable implantable delivery system of cefoperazone sodium. Implants were prepared by modified fabrication technique to avoid solvent use. Interaction studies were carried out to check any incompatibility between ingredients. Prepared implants were evaluated for various in vitro parameters like dimensions, hardness, tensile strength, drug release profile and sterility. Morphological changes in pellet before and after drug release were evaluated by scanning electron microscopy. The pellet were also tested for microbiological efficacy and compared with plain drug solution in different concentrations. Developed pellets are regular in shape and size with good tensile strength. The release profile displayed drug levels above MIC continuously up to 2 months. Wide zone of inhibition by pellet against Staph. aureus as compared to drug solution proves its efficacy in treatment of osteomyelitis.

No MeSH data available.


Related in: MedlinePlus