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Simultaneous spectrophotometric estimation of norfloxacin and ornidazole in tablet dosage form.

Wankhede SB, Prakash A, Kumari B, Chitlange SS - Indian J Pharm Sci (2009)

Bottom Line: Second method is Q-analysis method, based on absorbance ratio at two selected wavelengths 297.0 nm (iso-absorptive point) and 318.5 nm (lambda(max) of ornidazole).The linearity was obtained in the concentration range of 4-20 mug/ml and 5-25 mug/ml for norfloxacin and ornidazole, respectively.The results of the analysis have been validated statistically and by recovery studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Chemistry, Pad. Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Sant Tukaram Nagar, Pimpri, Pune-401 018, India.

ABSTRACT
Three simple, accurate and economical methods have been developed for the estimation of norfloxacin and ornidazole in tablet dosage form. First method is based on the simultaneous equations, wavelengths selected for analysis were 273.0 nm (lambda(max) of norfloxacin) and 318.5 nm (lambda(max) of ornidazole), respectively, in 0.1N NaOH. Second method is Q-analysis method, based on absorbance ratio at two selected wavelengths 297.0 nm (iso-absorptive point) and 318.5 nm (lambda(max) of ornidazole). Third method is first order derivative spectroscopy using 297.5 nm (zero cross for norfloxacin) and 264.0 nm (zero cross for ornidazole). The linearity was obtained in the concentration range of 4-20 mug/ml and 5-25 mug/ml for norfloxacin and ornidazole, respectively. The results of the analysis have been validated statistically and by recovery studies.

No MeSH data available.


Overlain spectra of norfloxacin and ornidazole.Overlain spectra of norfloxacin (NF, 273 nm) and ornidazole (OZ, 318.5 nm)
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Figure 0001: Overlain spectra of norfloxacin and ornidazole.Overlain spectra of norfloxacin (NF, 273 nm) and ornidazole (OZ, 318.5 nm)

Mentions: For the selection of analytical wavelength for the simultaneous equation method (Method-A), solutions of NF and OZ (20 μg/ml, each), were prepared separately by appropriate dilution of standard stock solution and scanned in the spectrum mode from 200 nm to 400 nm. From the overlain spectra of both drugs (fig. 1), wavelengths 273.0 nm (λmax of NF) and 318.5 nm (λmax of OZ) were selected for the simultaneous equations. The calibration curves for NF and OZ were prepared in the concentration range of 4-20 μg/ml and 5-25 μg/ml at both the wavelengths respectively. The absorptivity values were determined for both the drugs at both the wavelengths and following Eqns were used, A1 = 110.9CNF+15.3COZ (1) and A2 = 43.7CNF+28.4COZ (2), where A1 and A2 are absorbances of the sample at 273 nm and 318.5 nm, respectively, 110.9 and 43.7 are absorptivities of NF at 273.0 and 318.5 nm, respectively, 15.3 and 28.7 are the absorptivities of OZ at 273.0 nm and 318.5 nm, respectively. CNF is the concentration of NF and COZ is the concentration of the OZ. The mixture concentration was determined by using the Eqns. 1 and 2.


Simultaneous spectrophotometric estimation of norfloxacin and ornidazole in tablet dosage form.

Wankhede SB, Prakash A, Kumari B, Chitlange SS - Indian J Pharm Sci (2009)

Overlain spectra of norfloxacin and ornidazole.Overlain spectra of norfloxacin (NF, 273 nm) and ornidazole (OZ, 318.5 nm)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2865798&req=5

Figure 0001: Overlain spectra of norfloxacin and ornidazole.Overlain spectra of norfloxacin (NF, 273 nm) and ornidazole (OZ, 318.5 nm)
Mentions: For the selection of analytical wavelength for the simultaneous equation method (Method-A), solutions of NF and OZ (20 μg/ml, each), were prepared separately by appropriate dilution of standard stock solution and scanned in the spectrum mode from 200 nm to 400 nm. From the overlain spectra of both drugs (fig. 1), wavelengths 273.0 nm (λmax of NF) and 318.5 nm (λmax of OZ) were selected for the simultaneous equations. The calibration curves for NF and OZ were prepared in the concentration range of 4-20 μg/ml and 5-25 μg/ml at both the wavelengths respectively. The absorptivity values were determined for both the drugs at both the wavelengths and following Eqns were used, A1 = 110.9CNF+15.3COZ (1) and A2 = 43.7CNF+28.4COZ (2), where A1 and A2 are absorbances of the sample at 273 nm and 318.5 nm, respectively, 110.9 and 43.7 are absorptivities of NF at 273.0 and 318.5 nm, respectively, 15.3 and 28.7 are the absorptivities of OZ at 273.0 nm and 318.5 nm, respectively. CNF is the concentration of NF and COZ is the concentration of the OZ. The mixture concentration was determined by using the Eqns. 1 and 2.

Bottom Line: Second method is Q-analysis method, based on absorbance ratio at two selected wavelengths 297.0 nm (iso-absorptive point) and 318.5 nm (lambda(max) of ornidazole).The linearity was obtained in the concentration range of 4-20 mug/ml and 5-25 mug/ml for norfloxacin and ornidazole, respectively.The results of the analysis have been validated statistically and by recovery studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Chemistry, Pad. Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Sant Tukaram Nagar, Pimpri, Pune-401 018, India.

ABSTRACT
Three simple, accurate and economical methods have been developed for the estimation of norfloxacin and ornidazole in tablet dosage form. First method is based on the simultaneous equations, wavelengths selected for analysis were 273.0 nm (lambda(max) of norfloxacin) and 318.5 nm (lambda(max) of ornidazole), respectively, in 0.1N NaOH. Second method is Q-analysis method, based on absorbance ratio at two selected wavelengths 297.0 nm (iso-absorptive point) and 318.5 nm (lambda(max) of ornidazole). Third method is first order derivative spectroscopy using 297.5 nm (zero cross for norfloxacin) and 264.0 nm (zero cross for ornidazole). The linearity was obtained in the concentration range of 4-20 mug/ml and 5-25 mug/ml for norfloxacin and ornidazole, respectively. The results of the analysis have been validated statistically and by recovery studies.

No MeSH data available.