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Ion Pair-HPLC Method for the Simultaneous Estimation of Quinapril and Hydrochlorothiazide in Tablets.

Gandhimathi M, Ravi TK - Indian J Pharm Sci (2009)

Bottom Line: The detection of quinapril and hydrochlorothiazide was carried out on photo diode array detector at 220 nm.Results of the analysis were validated statistically and by recovery studies.The proposed method can be successfully used to determine the drug contents of marketed formulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Analysis, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, 395-Sarojini Naidu Road, New Siddhapudur, Coimbatore-641 044, India.

ABSTRACT
A simple, precise and rapid HPLC method has been developed and validated for the estimation of quinapril and hydrochlorothiazide simultaneously in combined dosage form. The mobile phase used was a mixture of 0.1% v/v triethylamine (pH 3.5), containing 1 mM of hexane sulphonic acid: acetonitrile (30:70% v/v). The detection of quinapril and hydrochlorothiazide was carried out on photo diode array detector at 220 nm. Results of the analysis were validated statistically and by recovery studies. The proposed method can be successfully used to determine the drug contents of marketed formulation.

No MeSH data available.


A typical chromatogram of HZ and QP.A typical chromatogram of hydrochlorothiazide (HZ) and quinapril (QP)
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Figure 0001: A typical chromatogram of HZ and QP.A typical chromatogram of hydrochlorothiazide (HZ) and quinapril (QP)

Mentions: Standard stock solutions 1 mg/ml of QP and 1.2 mg/ml of HZ were prepared in acetonitrile. For construction of calibration graph, working standard solution were prepared by further diluting the stock solution with mobile phase to get a concentration of 30-150 μg/ml of QP and 40-200 μg/ml of HZ. This method was applied to determine QP and HZ in combined formulation of market samples. For the analysis of tablet formulation an accurately weighed tablet powder equivalent to 10 mg of QP and 12.5 mg of HZ were taken in a volumetric flask. The powder was dissolved in acetonitrile, shaken thoroughly and made upto the volume with acetonitrile, Then the solution was filtered through Whatman filter paper No. 41 and further diluted with mobile phase to get the concentration of 90 μg/ml and 120 μg/ml of QP and HZ, respectively. These solutions were injected and the chromatograms were recorded. A typical chromatogram of HZ and QP is shown in fig 1.


Ion Pair-HPLC Method for the Simultaneous Estimation of Quinapril and Hydrochlorothiazide in Tablets.

Gandhimathi M, Ravi TK - Indian J Pharm Sci (2009)

A typical chromatogram of HZ and QP.A typical chromatogram of hydrochlorothiazide (HZ) and quinapril (QP)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2865793&req=5

Figure 0001: A typical chromatogram of HZ and QP.A typical chromatogram of hydrochlorothiazide (HZ) and quinapril (QP)
Mentions: Standard stock solutions 1 mg/ml of QP and 1.2 mg/ml of HZ were prepared in acetonitrile. For construction of calibration graph, working standard solution were prepared by further diluting the stock solution with mobile phase to get a concentration of 30-150 μg/ml of QP and 40-200 μg/ml of HZ. This method was applied to determine QP and HZ in combined formulation of market samples. For the analysis of tablet formulation an accurately weighed tablet powder equivalent to 10 mg of QP and 12.5 mg of HZ were taken in a volumetric flask. The powder was dissolved in acetonitrile, shaken thoroughly and made upto the volume with acetonitrile, Then the solution was filtered through Whatman filter paper No. 41 and further diluted with mobile phase to get the concentration of 90 μg/ml and 120 μg/ml of QP and HZ, respectively. These solutions were injected and the chromatograms were recorded. A typical chromatogram of HZ and QP is shown in fig 1.

Bottom Line: The detection of quinapril and hydrochlorothiazide was carried out on photo diode array detector at 220 nm.Results of the analysis were validated statistically and by recovery studies.The proposed method can be successfully used to determine the drug contents of marketed formulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Analysis, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, 395-Sarojini Naidu Road, New Siddhapudur, Coimbatore-641 044, India.

ABSTRACT
A simple, precise and rapid HPLC method has been developed and validated for the estimation of quinapril and hydrochlorothiazide simultaneously in combined dosage form. The mobile phase used was a mixture of 0.1% v/v triethylamine (pH 3.5), containing 1 mM of hexane sulphonic acid: acetonitrile (30:70% v/v). The detection of quinapril and hydrochlorothiazide was carried out on photo diode array detector at 220 nm. Results of the analysis were validated statistically and by recovery studies. The proposed method can be successfully used to determine the drug contents of marketed formulation.

No MeSH data available.