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Production of enterodiol from defatted flaxseeds through biotransformation by human intestinal bacteria.

Wang CZ, Ma XQ, Yang DH, Guo ZR, Liu GR, Zhao GX, Tang J, Zhang YN, Ma M, Cai SQ, Ku BS, Liu SL - BMC Microbiol. (2010)

Bottom Line: Based on analysis with pulsed field gel electrophoresis, END-49 was found to consist of five genomically distinct bacterial lineages, designated Group I-V, with Group I strains dominating the culture.Genomic analysis is under way to identify all members in END-49 involved in the biotransformation and the actual pathway leading to END-production.Biotransformation is a very economic, efficient and environmentally friendly way of mass-producing enterodiol from defatted flaxseeds.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology, Peking University Health Science Center, Beijing 100191, China.

ABSTRACT

Background: The effects of enterolignans, e.g., enterodiol (END) and particularly its oxidation product, enterolactone (ENL), on prevention of hormone-dependent diseases, such as osteoporosis, cardiovascular diseases, hyperlipemia, breast cancer, colon cancer, prostate cancer and menopausal syndrome, have attracted much attention. To date, the main way to obtain END and ENL is chemical synthesis, which is expensive and inevitably leads to environmental pollution. To explore a more economic and eco-friendly production method, we explored biotransformation of enterolignans from precursors contained in defatted flaxseeds by human intestinal bacteria.

Results: We cultured fecal specimens from healthy young adults in media containing defatted flaxseeds and detected END from the culture supernatant. Following selection through successive subcultures of the fecal microbiota with defatted flaxseeds as the only carbon source, we obtained a bacterial consortium, designated as END-49, which contained the smallest number of bacterial types still capable of metabolizing defatted flaxseeds to produce END. Based on analysis with pulsed field gel electrophoresis, END-49 was found to consist of five genomically distinct bacterial lineages, designated Group I-V, with Group I strains dominating the culture. None of the individual Group I-V strains produced END, demonstrating that the biotransformation of substrates in defatted flaxseeds into END is a joint work by different members of the END-49 bacterial consortium. Interestingly, Group I strains produced secoisolariciresinol, an important intermediate of END production; 16S rRNA analysis of one Group I strain established its close relatedness with Klebsiella. Genomic analysis is under way to identify all members in END-49 involved in the biotransformation and the actual pathway leading to END-production.

Conclusion: Biotransformation is a very economic, efficient and environmentally friendly way of mass-producing enterodiol from defatted flaxseeds.

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Biotransformation pathway of END and ENL from plant-derived lignan SDG; bacteria that work at different steps of the pathway, along with the authors who reported them, are indicated.
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Figure 1: Biotransformation pathway of END and ENL from plant-derived lignan SDG; bacteria that work at different steps of the pathway, along with the authors who reported them, are indicated.

Mentions: Biotransformation of SDG to END and ENL by human intestinal bacteria has been extensively studied, the pathway consisting of glycoside hydrolysis, demethylation, and dehydroxylation of SDG and its intermediates [9]. Bacteria that can produce END and ENL on plant lignans under strictly anaerobic conditions have been isolated from human feces [14-23] (Fig. 1). However, sufficient yields for marketing scale production of END and ENL by these microbes have not been achieved, largely due to the difficulty to create and maintain the strictly anaerobic culture conditions under which the bacteria can grow and conduct the biotransformation.


Production of enterodiol from defatted flaxseeds through biotransformation by human intestinal bacteria.

Wang CZ, Ma XQ, Yang DH, Guo ZR, Liu GR, Zhao GX, Tang J, Zhang YN, Ma M, Cai SQ, Ku BS, Liu SL - BMC Microbiol. (2010)

Biotransformation pathway of END and ENL from plant-derived lignan SDG; bacteria that work at different steps of the pathway, along with the authors who reported them, are indicated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2865466&req=5

Figure 1: Biotransformation pathway of END and ENL from plant-derived lignan SDG; bacteria that work at different steps of the pathway, along with the authors who reported them, are indicated.
Mentions: Biotransformation of SDG to END and ENL by human intestinal bacteria has been extensively studied, the pathway consisting of glycoside hydrolysis, demethylation, and dehydroxylation of SDG and its intermediates [9]. Bacteria that can produce END and ENL on plant lignans under strictly anaerobic conditions have been isolated from human feces [14-23] (Fig. 1). However, sufficient yields for marketing scale production of END and ENL by these microbes have not been achieved, largely due to the difficulty to create and maintain the strictly anaerobic culture conditions under which the bacteria can grow and conduct the biotransformation.

Bottom Line: Based on analysis with pulsed field gel electrophoresis, END-49 was found to consist of five genomically distinct bacterial lineages, designated Group I-V, with Group I strains dominating the culture.Genomic analysis is under way to identify all members in END-49 involved in the biotransformation and the actual pathway leading to END-production.Biotransformation is a very economic, efficient and environmentally friendly way of mass-producing enterodiol from defatted flaxseeds.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology, Peking University Health Science Center, Beijing 100191, China.

ABSTRACT

Background: The effects of enterolignans, e.g., enterodiol (END) and particularly its oxidation product, enterolactone (ENL), on prevention of hormone-dependent diseases, such as osteoporosis, cardiovascular diseases, hyperlipemia, breast cancer, colon cancer, prostate cancer and menopausal syndrome, have attracted much attention. To date, the main way to obtain END and ENL is chemical synthesis, which is expensive and inevitably leads to environmental pollution. To explore a more economic and eco-friendly production method, we explored biotransformation of enterolignans from precursors contained in defatted flaxseeds by human intestinal bacteria.

Results: We cultured fecal specimens from healthy young adults in media containing defatted flaxseeds and detected END from the culture supernatant. Following selection through successive subcultures of the fecal microbiota with defatted flaxseeds as the only carbon source, we obtained a bacterial consortium, designated as END-49, which contained the smallest number of bacterial types still capable of metabolizing defatted flaxseeds to produce END. Based on analysis with pulsed field gel electrophoresis, END-49 was found to consist of five genomically distinct bacterial lineages, designated Group I-V, with Group I strains dominating the culture. None of the individual Group I-V strains produced END, demonstrating that the biotransformation of substrates in defatted flaxseeds into END is a joint work by different members of the END-49 bacterial consortium. Interestingly, Group I strains produced secoisolariciresinol, an important intermediate of END production; 16S rRNA analysis of one Group I strain established its close relatedness with Klebsiella. Genomic analysis is under way to identify all members in END-49 involved in the biotransformation and the actual pathway leading to END-production.

Conclusion: Biotransformation is a very economic, efficient and environmentally friendly way of mass-producing enterodiol from defatted flaxseeds.

Show MeSH
Related in: MedlinePlus