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Gi- and Gq-coupled ADP (P2Y) receptors act in opposition to modulate nociceptive signaling and inflammatory pain behavior.

Malin SA, Molliver DC - Mol Pain (2010)

Bottom Line: Agonists for these receptors inhibit nociceptive signaling in isolated neurons and reduce behavioral hyperalgesia in vivo.Anti-nociceptive actions of these receptors appear to be antagonized by the Gq-coupled ADP receptor, P2Y1, which is required for the full expression of inflammatory hyperalgesia.Taken together, our data suggest that Gi-coupled P2Y receptors are broadly expressed in nociceptors, inhibit nociceptive signaling in vivo, and represent potential targets for the development of novel analgesic drugs.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dept Medicine; Dept Neurobiology, University of Pittsburgh, Pittsburgh, PA, USA. sachamalin@gmail.com

ABSTRACT

Background: Investigations of nucleotide signaling in nociception to date have focused on actions of adenosine triphosphate (ATP). Both ATP-gated ion channels (P2X receptors) and G protein-coupled (P2Y) receptors contribute to nociceptive signaling in peripheral sensory neurons. In addition, several studies have implicated the Gq-coupled adenosine diphosphate (ADP) receptor P2Y1 in sensory transduction. In this study, we examined the expression and function of P2Y1 and the Gi-coupled receptors P2Y12, P2Y13 and P2Y14 in sensory neurons to determine their contribution to nociception.

Results: We detected mRNA and protein for ADP receptors P2Y12 and P2Y13 in mouse dorsal root ganglia (DRG). P2Y14, a homologous Gi-coupled nucleotide receptor, is also expressed in DRG. Immunohistochemical analysis of receptor distribution indicated that these receptors are widely expressed in nociceptive neurons. Using ratiometric calcium imaging, we found that ADP evokes increases in intracellular calcium in isolated DRG neurons and also produces a pertussis toxin-sensitive inhibition of depolarization-evoked calcium transients. The inhibitory effect of ADP was unaltered in the presence of the selective P2Y1 antagonist MRS2179 and in neurons isolated from P2Y1 knockout mice, whereas ADP-evoked calcium transients were greatly reduced. Analysis of behavioral responses to noxious heat before and after inflammatory injury (injection of complete Freund's adjuvant into the hindpaw) revealed that P2Y1 is required for the full expression of inflammatory hyperalgesia, whereas local injection of agonists for Gi-coupled P2Y receptors reduced hyperalgesia.

Conclusions: We report that Gi-coupled P2Y receptors are widely expressed in peripheral sensory neurons. Agonists for these receptors inhibit nociceptive signaling in isolated neurons and reduce behavioral hyperalgesia in vivo. Anti-nociceptive actions of these receptors appear to be antagonized by the Gq-coupled ADP receptor, P2Y1, which is required for the full expression of inflammatory hyperalgesia. We propose that nociceptor sensitivity is modulated by the integration of nucleotide signaling through Gq- and Gi-coupled P2Y receptors, and this balance is altered in response to inflammatory injury. Taken together, our data suggest that Gi-coupled P2Y receptors are broadly expressed in nociceptors, inhibit nociceptive signaling in vivo, and represent potential targets for the development of novel analgesic drugs.

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P2YGi agonists inhibit depolarization-evoked increases in intracellular Ca++. Fura-2 Ca++ imaging was used to measure the effect of P2Y receptor agonists on Ca++ transients evoked by administration of 50 mM K+. Application of ADP (100 μM) for 3 minutes reduced subsequent depolarization-evoked transients in wildtype (A) and P2Y1-/- (B) mice. The P2Y13 agonist IDP (C) and the P2Y14 agonist UDPG (D) also inhibited depolarization-evoked transients.
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Figure 3: P2YGi agonists inhibit depolarization-evoked increases in intracellular Ca++. Fura-2 Ca++ imaging was used to measure the effect of P2Y receptor agonists on Ca++ transients evoked by administration of 50 mM K+. Application of ADP (100 μM) for 3 minutes reduced subsequent depolarization-evoked transients in wildtype (A) and P2Y1-/- (B) mice. The P2Y13 agonist IDP (C) and the P2Y14 agonist UDPG (D) also inhibited depolarization-evoked transients.

Mentions: To examine the actions of the P2YGi receptors, we first tested the ability of ADP to inhibit Ca++ transients evoked by a depolarizing stimulus of 50 mM K+. ADP (100 μM) inhibited both the magnitude and the duration of depolarization-evoked transients in the majority of neurons in vitro (Figure 3A). ADP was equally effective in neurons from P2Y1-/- mice, ruling out an essential role for P2Y1 (Figure 3B). The inhibitory effect of ADP was lost when cells were pretreated overnight with 250 nM pertussis toxin, indicating that inhibition was mediated through a Gi-coupled pathway (n = 3 mice, 32 cells, data not shown). IDP and UDPG also inhibited depolarization-evoked Ca++ transients (Figure 3C-D; Table 3).


Gi- and Gq-coupled ADP (P2Y) receptors act in opposition to modulate nociceptive signaling and inflammatory pain behavior.

Malin SA, Molliver DC - Mol Pain (2010)

P2YGi agonists inhibit depolarization-evoked increases in intracellular Ca++. Fura-2 Ca++ imaging was used to measure the effect of P2Y receptor agonists on Ca++ transients evoked by administration of 50 mM K+. Application of ADP (100 μM) for 3 minutes reduced subsequent depolarization-evoked transients in wildtype (A) and P2Y1-/- (B) mice. The P2Y13 agonist IDP (C) and the P2Y14 agonist UDPG (D) also inhibited depolarization-evoked transients.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2865444&req=5

Figure 3: P2YGi agonists inhibit depolarization-evoked increases in intracellular Ca++. Fura-2 Ca++ imaging was used to measure the effect of P2Y receptor agonists on Ca++ transients evoked by administration of 50 mM K+. Application of ADP (100 μM) for 3 minutes reduced subsequent depolarization-evoked transients in wildtype (A) and P2Y1-/- (B) mice. The P2Y13 agonist IDP (C) and the P2Y14 agonist UDPG (D) also inhibited depolarization-evoked transients.
Mentions: To examine the actions of the P2YGi receptors, we first tested the ability of ADP to inhibit Ca++ transients evoked by a depolarizing stimulus of 50 mM K+. ADP (100 μM) inhibited both the magnitude and the duration of depolarization-evoked transients in the majority of neurons in vitro (Figure 3A). ADP was equally effective in neurons from P2Y1-/- mice, ruling out an essential role for P2Y1 (Figure 3B). The inhibitory effect of ADP was lost when cells were pretreated overnight with 250 nM pertussis toxin, indicating that inhibition was mediated through a Gi-coupled pathway (n = 3 mice, 32 cells, data not shown). IDP and UDPG also inhibited depolarization-evoked Ca++ transients (Figure 3C-D; Table 3).

Bottom Line: Agonists for these receptors inhibit nociceptive signaling in isolated neurons and reduce behavioral hyperalgesia in vivo.Anti-nociceptive actions of these receptors appear to be antagonized by the Gq-coupled ADP receptor, P2Y1, which is required for the full expression of inflammatory hyperalgesia.Taken together, our data suggest that Gi-coupled P2Y receptors are broadly expressed in nociceptors, inhibit nociceptive signaling in vivo, and represent potential targets for the development of novel analgesic drugs.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dept Medicine; Dept Neurobiology, University of Pittsburgh, Pittsburgh, PA, USA. sachamalin@gmail.com

ABSTRACT

Background: Investigations of nucleotide signaling in nociception to date have focused on actions of adenosine triphosphate (ATP). Both ATP-gated ion channels (P2X receptors) and G protein-coupled (P2Y) receptors contribute to nociceptive signaling in peripheral sensory neurons. In addition, several studies have implicated the Gq-coupled adenosine diphosphate (ADP) receptor P2Y1 in sensory transduction. In this study, we examined the expression and function of P2Y1 and the Gi-coupled receptors P2Y12, P2Y13 and P2Y14 in sensory neurons to determine their contribution to nociception.

Results: We detected mRNA and protein for ADP receptors P2Y12 and P2Y13 in mouse dorsal root ganglia (DRG). P2Y14, a homologous Gi-coupled nucleotide receptor, is also expressed in DRG. Immunohistochemical analysis of receptor distribution indicated that these receptors are widely expressed in nociceptive neurons. Using ratiometric calcium imaging, we found that ADP evokes increases in intracellular calcium in isolated DRG neurons and also produces a pertussis toxin-sensitive inhibition of depolarization-evoked calcium transients. The inhibitory effect of ADP was unaltered in the presence of the selective P2Y1 antagonist MRS2179 and in neurons isolated from P2Y1 knockout mice, whereas ADP-evoked calcium transients were greatly reduced. Analysis of behavioral responses to noxious heat before and after inflammatory injury (injection of complete Freund's adjuvant into the hindpaw) revealed that P2Y1 is required for the full expression of inflammatory hyperalgesia, whereas local injection of agonists for Gi-coupled P2Y receptors reduced hyperalgesia.

Conclusions: We report that Gi-coupled P2Y receptors are widely expressed in peripheral sensory neurons. Agonists for these receptors inhibit nociceptive signaling in isolated neurons and reduce behavioral hyperalgesia in vivo. Anti-nociceptive actions of these receptors appear to be antagonized by the Gq-coupled ADP receptor, P2Y1, which is required for the full expression of inflammatory hyperalgesia. We propose that nociceptor sensitivity is modulated by the integration of nucleotide signaling through Gq- and Gi-coupled P2Y receptors, and this balance is altered in response to inflammatory injury. Taken together, our data suggest that Gi-coupled P2Y receptors are broadly expressed in nociceptors, inhibit nociceptive signaling in vivo, and represent potential targets for the development of novel analgesic drugs.

Show MeSH
Related in: MedlinePlus