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Host specificity and speciation of Mycosphaerella and Teratosphaeria species associated with leaf spots of Proteaceae.

Crous PW, Summerell BA, Mostert L, Groenewald JZ - Persoonia (2008)

Bottom Line: Although accepted as being highly host specific, some species were shown to have wider host ranges, such as M. communis (Eucalyptus, Protea), M. konae (Leucospermum, Eucalyptus), M. marksii (Eucalyptus, Leucadendron), T. associata (Eucalyptus,Protea), and T. parva (Eucalyptus, Protea), which in most cases were found to co-occur with other species of Mycosphaerella or Teratosphaeria on Proteaceae.A phenomenon of underdeveloped, or micro-ascospores was also newly observed in asci of T. maculiformis and T. proteae-arboreae.The exact purpose of asci with two distinct types of ascospores remains to be clarified, as both types were observed to germinate on agar.

View Article: PubMed Central - PubMed

Affiliation: CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands;

ABSTRACT
Species of Mycosphaerella and Teratosphaeria represent important foliicolous pathogens of Proteaceae. Presently approximately 40 members of these genera (incl. anamorphs) have been recorded from Proteaceae, though the majority are not known from culture, and have never been subjected to DNA sequence analysis. During the course of this study, epitypes were designated for several important species, namely Batcheloromyces leucadendri, B. proteae, Catenulostroma macowanii, Mycosphaerella marksii, Teratosphaeria bellula, T. jonkershoekensis, T. parva, and T. proteae-arboreae. Several species were also newly described, namely Batcheloromyces sedgefieldii, Catenulostroma wingfieldii, Dissoconium proteae, Teratosphaeria persoonii, T. knoxdavesii, and T. marasasii. Although accepted as being highly host specific, some species were shown to have wider host ranges, such as M. communis (Eucalyptus, Protea), M. konae (Leucospermum, Eucalyptus), M. marksii (Eucalyptus, Leucadendron), T. associata (Eucalyptus,Protea), and T. parva (Eucalyptus, Protea), which in most cases were found to co-occur with other species of Mycosphaerella or Teratosphaeria on Proteaceae. Furthermore, earlier records of T. jonkershoekensis on Proteaceae in Australia were shown to be representative of two recently described species, T. associata and T. maxii. A phenomenon of underdeveloped, or micro-ascospores was also newly observed in asci of T. maculiformis and T. proteae-arboreae. The exact purpose of asci with two distinct types of ascospores remains to be clarified, as both types were observed to germinate on agar.

No MeSH data available.


Related in: MedlinePlus

One of 10 000 equally most parsimonious trees obtained from a heuristic search with 100 random taxon additions of the ITS sequence alignment using PAUP v. 4.0b10. The scale bar shows 10 changes and bootstrap support values from 10 000 000 fast stepwise replicates are shown at the nodes. Thickened lines indicate the strict consensus branches and ex-type sequences are printed in bold face. The tree was rooted to a sequence obtained from GenBank (Cladoriella eucalypti EU040224).
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Figure 1: One of 10 000 equally most parsimonious trees obtained from a heuristic search with 100 random taxon additions of the ITS sequence alignment using PAUP v. 4.0b10. The scale bar shows 10 changes and bootstrap support values from 10 000 000 fast stepwise replicates are shown at the nodes. Thickened lines indicate the strict consensus branches and ex-type sequences are printed in bold face. The tree was rooted to a sequence obtained from GenBank (Cladoriella eucalypti EU040224).

Mentions: Amplicons of approximately 1 700 bases were obtained for the isolates listed in Table 1. The ITS sequences were used to obtain additional sequences from GenBank, which were added to the alignment. The manually adjusted ITS alignment contained 151 sequences (including the outgroup sequence) and 973 characters including alignment gaps (available in TreeBASE). Of the 540 characters used in the phylogenetic analysis, 279 were parsimony-informative, 55 were variable and parsimony-uninformative, and 206 were constant. Neighbour-joining analyses using three substitution models on the sequence alignment yielded trees with identical topologies to one another, except for the position of Capnobotryella renispora, which differed when the uncorrected ā€˜pā€™ substitution model was compared with the Kimura 2-parameter and HKY85 models (it is placed basal to Teratosphaeria and Mycosphaerella in the latter two models). The neighbour-joining trees support the same clades as obtained from the parsimony analysis, but with a different arrangement at the deep nodes, for example the position of the Dissoconium and Phaeotheca clades. Because of the large number of different strain associations, for example in the Mycosphaerella marksii, Pseudocercospora and Teratosphaeria parva clades (as evident from the strict consensus branches shown in Fig. 1), only the first 10 000 equally most parsimonious trees (TL = 1725 steps; CI = 0.386; RI = 0.870; RC = 0.336) were saved, one of which is shown in Fig. 1. The phylogenetic results obtained are discussed where applicable in the descriptive notes below.


Host specificity and speciation of Mycosphaerella and Teratosphaeria species associated with leaf spots of Proteaceae.

Crous PW, Summerell BA, Mostert L, Groenewald JZ - Persoonia (2008)

One of 10 000 equally most parsimonious trees obtained from a heuristic search with 100 random taxon additions of the ITS sequence alignment using PAUP v. 4.0b10. The scale bar shows 10 changes and bootstrap support values from 10 000 000 fast stepwise replicates are shown at the nodes. Thickened lines indicate the strict consensus branches and ex-type sequences are printed in bold face. The tree was rooted to a sequence obtained from GenBank (Cladoriella eucalypti EU040224).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2865354&req=5

Figure 1: One of 10 000 equally most parsimonious trees obtained from a heuristic search with 100 random taxon additions of the ITS sequence alignment using PAUP v. 4.0b10. The scale bar shows 10 changes and bootstrap support values from 10 000 000 fast stepwise replicates are shown at the nodes. Thickened lines indicate the strict consensus branches and ex-type sequences are printed in bold face. The tree was rooted to a sequence obtained from GenBank (Cladoriella eucalypti EU040224).
Mentions: Amplicons of approximately 1 700 bases were obtained for the isolates listed in Table 1. The ITS sequences were used to obtain additional sequences from GenBank, which were added to the alignment. The manually adjusted ITS alignment contained 151 sequences (including the outgroup sequence) and 973 characters including alignment gaps (available in TreeBASE). Of the 540 characters used in the phylogenetic analysis, 279 were parsimony-informative, 55 were variable and parsimony-uninformative, and 206 were constant. Neighbour-joining analyses using three substitution models on the sequence alignment yielded trees with identical topologies to one another, except for the position of Capnobotryella renispora, which differed when the uncorrected ā€˜pā€™ substitution model was compared with the Kimura 2-parameter and HKY85 models (it is placed basal to Teratosphaeria and Mycosphaerella in the latter two models). The neighbour-joining trees support the same clades as obtained from the parsimony analysis, but with a different arrangement at the deep nodes, for example the position of the Dissoconium and Phaeotheca clades. Because of the large number of different strain associations, for example in the Mycosphaerella marksii, Pseudocercospora and Teratosphaeria parva clades (as evident from the strict consensus branches shown in Fig. 1), only the first 10 000 equally most parsimonious trees (TL = 1725 steps; CI = 0.386; RI = 0.870; RC = 0.336) were saved, one of which is shown in Fig. 1. The phylogenetic results obtained are discussed where applicable in the descriptive notes below.

Bottom Line: Although accepted as being highly host specific, some species were shown to have wider host ranges, such as M. communis (Eucalyptus, Protea), M. konae (Leucospermum, Eucalyptus), M. marksii (Eucalyptus, Leucadendron), T. associata (Eucalyptus,Protea), and T. parva (Eucalyptus, Protea), which in most cases were found to co-occur with other species of Mycosphaerella or Teratosphaeria on Proteaceae.A phenomenon of underdeveloped, or micro-ascospores was also newly observed in asci of T. maculiformis and T. proteae-arboreae.The exact purpose of asci with two distinct types of ascospores remains to be clarified, as both types were observed to germinate on agar.

View Article: PubMed Central - PubMed

Affiliation: CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands;

ABSTRACT
Species of Mycosphaerella and Teratosphaeria represent important foliicolous pathogens of Proteaceae. Presently approximately 40 members of these genera (incl. anamorphs) have been recorded from Proteaceae, though the majority are not known from culture, and have never been subjected to DNA sequence analysis. During the course of this study, epitypes were designated for several important species, namely Batcheloromyces leucadendri, B. proteae, Catenulostroma macowanii, Mycosphaerella marksii, Teratosphaeria bellula, T. jonkershoekensis, T. parva, and T. proteae-arboreae. Several species were also newly described, namely Batcheloromyces sedgefieldii, Catenulostroma wingfieldii, Dissoconium proteae, Teratosphaeria persoonii, T. knoxdavesii, and T. marasasii. Although accepted as being highly host specific, some species were shown to have wider host ranges, such as M. communis (Eucalyptus, Protea), M. konae (Leucospermum, Eucalyptus), M. marksii (Eucalyptus, Leucadendron), T. associata (Eucalyptus,Protea), and T. parva (Eucalyptus, Protea), which in most cases were found to co-occur with other species of Mycosphaerella or Teratosphaeria on Proteaceae. Furthermore, earlier records of T. jonkershoekensis on Proteaceae in Australia were shown to be representative of two recently described species, T. associata and T. maxii. A phenomenon of underdeveloped, or micro-ascospores was also newly observed in asci of T. maculiformis and T. proteae-arboreae. The exact purpose of asci with two distinct types of ascospores remains to be clarified, as both types were observed to germinate on agar.

No MeSH data available.


Related in: MedlinePlus