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A phylogenetic study of Boletus section Boletus in Europe.

Beugelsdijk DC, van der Linde S, Zuccarello GC, den Bakker HC, Draisma SG, Noordeloos ME - Persoonia (2008)

Bottom Line: The ML and MP trees of ITS showed remarkably low resolution within the B. edulis clade, and confirmed earlier published results, despite the use of samples from a wider geographical area and different hosts.The results of GAPDH demonstrate clearly that this low resolution must be ascribed to a low genetic variability with the B. edulis clade, and make clear that morphological and ecological characters have been overestimated within this species complex.Three other European species (B. aereus, B. pinophilus and B. reticulatus) are well delimited species based on morphology and our genetic data.

View Article: PubMed Central - PubMed

Affiliation: National Herbarium of the Netherlands, Leiden University branch, P.O. Box 9514, 2300 RA Leiden, The Netherlands;

ABSTRACT
A phylogenetic study of the species in Boletus sect. Boletus was undertaken using the molecular markers ITS1-5.8S-ITS2 and GAPDH. Four well-supported lineages, one comprising Boletus edulis s.l., the others referring to B. aereus, B. reticulatus and B. pinophilus have been distinguished. The ML and MP trees of ITS showed remarkably low resolution within the B. edulis clade, and confirmed earlier published results, despite the use of samples from a wider geographical area and different hosts. The results of GAPDH demonstrate clearly that this low resolution must be ascribed to a low genetic variability with the B. edulis clade, and make clear that morphological and ecological characters have been overestimated within this species complex. Boletus edulis is therefore defined as a variable species with a wide morphological, ecological and geographic range, and includes several specific and subspecific taxa described in the literature (e.g. B. betulicola, B. persoonii, B. quercicola and B. venturii). Three other European species (B. aereus, B. pinophilus and B. reticulatus) are well delimited species based on morphology and our genetic data.

No MeSH data available.


Related in: MedlinePlus

Maximum likelihood tree from the combined ITS and GAPDH data. Bolletus fechtneri is selected as the outgroup. Above the branches bootstrap support values are displayed based on maximum parsimony (percentage), distance (percentage) and posterior probability (frequency) respectively. Behind the name and number of the samples the collection location and host genus are shown. NL = The Netherlands, FIN = Finland, S = Sweden, B = Belgium, A = Austria. ■ = Picea, ♦ = Pinus, * = Quercus, ○ = Betula, ▵ = Fagus, ▿ = Tilia, + = Castanea.
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Figure 1: Maximum likelihood tree from the combined ITS and GAPDH data. Bolletus fechtneri is selected as the outgroup. Above the branches bootstrap support values are displayed based on maximum parsimony (percentage), distance (percentage) and posterior probability (frequency) respectively. Behind the name and number of the samples the collection location and host genus are shown. NL = The Netherlands, FIN = Finland, S = Sweden, B = Belgium, A = Austria. ■ = Picea, ♦ = Pinus, * = Quercus, ○ = Betula, ▵ = Fagus, ▿ = Tilia, + = Castanea.

Mentions: The ITS and GAPDH alignment consisted of 860 and 496 characters respectively of which 57 and 39 were parsimony informative. The partition homogeneity test indicated that the two molecular data sets (ITS and GAPDH) could be combined (P = 0.560). This alignment consisted of 1356 basepairs (bp) and contained 96 parsimony-informative sites. The MP analysis produced 6 600 most parsimonious trees of 305 steps. The strict consensus tree of the MP trees is congruent to the ML tree (Fig. 1).


A phylogenetic study of Boletus section Boletus in Europe.

Beugelsdijk DC, van der Linde S, Zuccarello GC, den Bakker HC, Draisma SG, Noordeloos ME - Persoonia (2008)

Maximum likelihood tree from the combined ITS and GAPDH data. Bolletus fechtneri is selected as the outgroup. Above the branches bootstrap support values are displayed based on maximum parsimony (percentage), distance (percentage) and posterior probability (frequency) respectively. Behind the name and number of the samples the collection location and host genus are shown. NL = The Netherlands, FIN = Finland, S = Sweden, B = Belgium, A = Austria. ■ = Picea, ♦ = Pinus, * = Quercus, ○ = Betula, ▵ = Fagus, ▿ = Tilia, + = Castanea.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2865352&req=5

Figure 1: Maximum likelihood tree from the combined ITS and GAPDH data. Bolletus fechtneri is selected as the outgroup. Above the branches bootstrap support values are displayed based on maximum parsimony (percentage), distance (percentage) and posterior probability (frequency) respectively. Behind the name and number of the samples the collection location and host genus are shown. NL = The Netherlands, FIN = Finland, S = Sweden, B = Belgium, A = Austria. ■ = Picea, ♦ = Pinus, * = Quercus, ○ = Betula, ▵ = Fagus, ▿ = Tilia, + = Castanea.
Mentions: The ITS and GAPDH alignment consisted of 860 and 496 characters respectively of which 57 and 39 were parsimony informative. The partition homogeneity test indicated that the two molecular data sets (ITS and GAPDH) could be combined (P = 0.560). This alignment consisted of 1356 basepairs (bp) and contained 96 parsimony-informative sites. The MP analysis produced 6 600 most parsimonious trees of 305 steps. The strict consensus tree of the MP trees is congruent to the ML tree (Fig. 1).

Bottom Line: The ML and MP trees of ITS showed remarkably low resolution within the B. edulis clade, and confirmed earlier published results, despite the use of samples from a wider geographical area and different hosts.The results of GAPDH demonstrate clearly that this low resolution must be ascribed to a low genetic variability with the B. edulis clade, and make clear that morphological and ecological characters have been overestimated within this species complex.Three other European species (B. aereus, B. pinophilus and B. reticulatus) are well delimited species based on morphology and our genetic data.

View Article: PubMed Central - PubMed

Affiliation: National Herbarium of the Netherlands, Leiden University branch, P.O. Box 9514, 2300 RA Leiden, The Netherlands;

ABSTRACT
A phylogenetic study of the species in Boletus sect. Boletus was undertaken using the molecular markers ITS1-5.8S-ITS2 and GAPDH. Four well-supported lineages, one comprising Boletus edulis s.l., the others referring to B. aereus, B. reticulatus and B. pinophilus have been distinguished. The ML and MP trees of ITS showed remarkably low resolution within the B. edulis clade, and confirmed earlier published results, despite the use of samples from a wider geographical area and different hosts. The results of GAPDH demonstrate clearly that this low resolution must be ascribed to a low genetic variability with the B. edulis clade, and make clear that morphological and ecological characters have been overestimated within this species complex. Boletus edulis is therefore defined as a variable species with a wide morphological, ecological and geographic range, and includes several specific and subspecific taxa described in the literature (e.g. B. betulicola, B. persoonii, B. quercicola and B. venturii). Three other European species (B. aereus, B. pinophilus and B. reticulatus) are well delimited species based on morphology and our genetic data.

No MeSH data available.


Related in: MedlinePlus