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Multiple gene genealogies and phenotypic characters differentiate several novel species of Mycosphaerella and related anamorphs on banana.

Arzanlou M, Groenewald JZ, Fullerton RA, Abeln EC, Carlier J, Zapater MF, Buddenhagen IW, Viljoen A, Crous PW - Persoonia (2008)

Bottom Line: In the present study, we collected a global set of Mycosphaerella strains from banana, and compared them by means of morphology and a multi-gene nucleotide sequence data set.This study reconfirmed the previously described presence of Cercospora apii, M. citri and M. thailandica, and also identified Mycosphaerella communis, M. lateralis and Passalora loranthi on this host.Moreover, eight new species identified from Musa are described, namely Dissoconium musae, Mycosphaerella mozambica, Pseudocercospora assamensis, P. indonesiana, P. longispora, Stenella musae, S. musicola, and S. queenslandica.

View Article: PubMed Central - PubMed

Affiliation: CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands;

ABSTRACT
Three species of Mycosphaerella, namely M. eumusae, M. fijiensis, and M. musicola are involved in the Sigatoka disease complex of bananas. Besides these three primary pathogens, several additional species of Mycosphaerella or their anamorphs have been described from Musa. However, very little is known about these taxa, and for the majority of these species no culture or DNA is available for study. In the present study, we collected a global set of Mycosphaerella strains from banana, and compared them by means of morphology and a multi-gene nucleotide sequence data set. The phylogeny inferred from the ITS region and the combined data set containing partial gene sequences of the actin gene, the small subunit mitochondrial ribosomal DNA and the histone H3 gene revealed a rich diversity of Mycosphaerella species on Musa. Integration of morphological and molecular data sets confirmed more than 20 species of Mycosphaerella (incl. anamorphs) to occur on banana. This study reconfirmed the previously described presence of Cercospora apii, M. citri and M. thailandica, and also identified Mycosphaerella communis, M. lateralis and Passalora loranthi on this host. Moreover, eight new species identified from Musa are described, namely Dissoconium musae, Mycosphaerella mozambica, Pseudocercospora assamensis, P. indonesiana, P. longispora, Stenella musae, S. musicola, and S. queenslandica.

No MeSH data available.


Related in: MedlinePlus

One of eight equally most parsimonious trees obtained from a heuristic search with 100 random taxon additions of the combined (ITS, ACT, HIS, mtSSU) sequence alignment. The scale bar shows 10 changes, and bootstrap support values (65 % and higher) from 1000 replicates are shown at the nodes. Thickened lines indicate the strict consensus branches. The tree was rooted to sequences of Davidiella tassiana strain CPC 11600 (GenBank accession number DQ289800, DQ289867, EF679665, EU514455, respectively). M. = Mycosphaerella.
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Figure 2: One of eight equally most parsimonious trees obtained from a heuristic search with 100 random taxon additions of the combined (ITS, ACT, HIS, mtSSU) sequence alignment. The scale bar shows 10 changes, and bootstrap support values (65 % and higher) from 1000 replicates are shown at the nodes. Thickened lines indicate the strict consensus branches. The tree was rooted to sequences of Davidiella tassiana strain CPC 11600 (GenBank accession number DQ289800, DQ289867, EF679665, EU514455, respectively). M. = Mycosphaerella.

Mentions: The sequence data in the second alignment were analysed as one combined set consisting of 1648 characters (incl. alignment gaps) (number of included characters: ITS: 509, ACT: 188, HIS: 375, mtSSU: 576). This second alignment included 54 sequences (including the outgroup) and of the 1648 characters, 517 were parsimony-informative, 93 were variable and parsimony-uninformative, and 1038 were constant. Trees supporting the same clades were obtained irrespective of the analysis method used. The parsimony analysis yielded eight equally most parsimonious trees that mainly differed in the order of taxa at the terminal nodes; one of the trees is presented in Fig. 2 (TL = 1513 steps; CI = 0.654; RI = 0.901; RC = 0.589). Similar to the results obtained for the ITS alignment, the same lineages were found with the combined alignment. The ACT and HIS data were found to be more variable within species than the ITS and mtSSU data (data not shown for individual loci, variation within clades in Fig. 2). The phylogenetic results obtained are discussed where applicable in the descriptive notes below.


Multiple gene genealogies and phenotypic characters differentiate several novel species of Mycosphaerella and related anamorphs on banana.

Arzanlou M, Groenewald JZ, Fullerton RA, Abeln EC, Carlier J, Zapater MF, Buddenhagen IW, Viljoen A, Crous PW - Persoonia (2008)

One of eight equally most parsimonious trees obtained from a heuristic search with 100 random taxon additions of the combined (ITS, ACT, HIS, mtSSU) sequence alignment. The scale bar shows 10 changes, and bootstrap support values (65 % and higher) from 1000 replicates are shown at the nodes. Thickened lines indicate the strict consensus branches. The tree was rooted to sequences of Davidiella tassiana strain CPC 11600 (GenBank accession number DQ289800, DQ289867, EF679665, EU514455, respectively). M. = Mycosphaerella.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2865351&req=5

Figure 2: One of eight equally most parsimonious trees obtained from a heuristic search with 100 random taxon additions of the combined (ITS, ACT, HIS, mtSSU) sequence alignment. The scale bar shows 10 changes, and bootstrap support values (65 % and higher) from 1000 replicates are shown at the nodes. Thickened lines indicate the strict consensus branches. The tree was rooted to sequences of Davidiella tassiana strain CPC 11600 (GenBank accession number DQ289800, DQ289867, EF679665, EU514455, respectively). M. = Mycosphaerella.
Mentions: The sequence data in the second alignment were analysed as one combined set consisting of 1648 characters (incl. alignment gaps) (number of included characters: ITS: 509, ACT: 188, HIS: 375, mtSSU: 576). This second alignment included 54 sequences (including the outgroup) and of the 1648 characters, 517 were parsimony-informative, 93 were variable and parsimony-uninformative, and 1038 were constant. Trees supporting the same clades were obtained irrespective of the analysis method used. The parsimony analysis yielded eight equally most parsimonious trees that mainly differed in the order of taxa at the terminal nodes; one of the trees is presented in Fig. 2 (TL = 1513 steps; CI = 0.654; RI = 0.901; RC = 0.589). Similar to the results obtained for the ITS alignment, the same lineages were found with the combined alignment. The ACT and HIS data were found to be more variable within species than the ITS and mtSSU data (data not shown for individual loci, variation within clades in Fig. 2). The phylogenetic results obtained are discussed where applicable in the descriptive notes below.

Bottom Line: In the present study, we collected a global set of Mycosphaerella strains from banana, and compared them by means of morphology and a multi-gene nucleotide sequence data set.This study reconfirmed the previously described presence of Cercospora apii, M. citri and M. thailandica, and also identified Mycosphaerella communis, M. lateralis and Passalora loranthi on this host.Moreover, eight new species identified from Musa are described, namely Dissoconium musae, Mycosphaerella mozambica, Pseudocercospora assamensis, P. indonesiana, P. longispora, Stenella musae, S. musicola, and S. queenslandica.

View Article: PubMed Central - PubMed

Affiliation: CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands;

ABSTRACT
Three species of Mycosphaerella, namely M. eumusae, M. fijiensis, and M. musicola are involved in the Sigatoka disease complex of bananas. Besides these three primary pathogens, several additional species of Mycosphaerella or their anamorphs have been described from Musa. However, very little is known about these taxa, and for the majority of these species no culture or DNA is available for study. In the present study, we collected a global set of Mycosphaerella strains from banana, and compared them by means of morphology and a multi-gene nucleotide sequence data set. The phylogeny inferred from the ITS region and the combined data set containing partial gene sequences of the actin gene, the small subunit mitochondrial ribosomal DNA and the histone H3 gene revealed a rich diversity of Mycosphaerella species on Musa. Integration of morphological and molecular data sets confirmed more than 20 species of Mycosphaerella (incl. anamorphs) to occur on banana. This study reconfirmed the previously described presence of Cercospora apii, M. citri and M. thailandica, and also identified Mycosphaerella communis, M. lateralis and Passalora loranthi on this host. Moreover, eight new species identified from Musa are described, namely Dissoconium musae, Mycosphaerella mozambica, Pseudocercospora assamensis, P. indonesiana, P. longispora, Stenella musae, S. musicola, and S. queenslandica.

No MeSH data available.


Related in: MedlinePlus