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Morphological and molecular characterisation of a new anamorphic genus Cheirosporium, from freshwater in China.

Cai L, Guo XY, Hyde KD - Persoonia (2008)

Bottom Line: The 28S rDNA and ITS rDNA operon of this taxon were amplified and sequenced.Phylogenetic analysis showed that C. triseriale groups with Dictyosporium and allied species, and nests within the Pleosporales (Dothideomycetes, Ascomycota).Cheirosporium is morphologically distinct from the cheirosporous genera Cheiromyces, Cheiromycina, Dictyosporium, Digitomyces, Digitodesmium and Pseudodictyosporium and these differences are discussed.

View Article: PubMed Central - PubMed

Affiliation: Centre for Research in Fungal Diversity, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong;

ABSTRACT
Cheirosporium gen. nov. is characterised by the production of sporodochial conidiomata, semi-macronematous to macronematous conidiophores that possess several distinct sterile branches, and cheiroid, smooth-walled conidia with rhexolytic secession. The 28S rDNA and ITS rDNA operon of this taxon were amplified and sequenced. A BLAST search revealed low homology between Cheirosporium triseriale and existing sequences in public databases, supporting the hypothesis that the species is new to science. Phylogenetic analysis showed that C. triseriale groups with Dictyosporium and allied species, and nests within the Pleosporales (Dothideomycetes, Ascomycota). Cheirosporium is morphologically distinct from the cheirosporous genera Cheiromyces, Cheiromycina, Dictyosporium, Digitomyces, Digitodesmium and Pseudodictyosporium and these differences are discussed.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree generated from parsimony analysis based on 28S rDNA sequences. Data were analysed with random addition sequence, and treating gaps as missing data. Bootstrap values ≥ 50 % are shown above or below branches. Thickened branches indicate Bayesian posterior probabilities ≥ 95 %. The tree is rooted with Aniptodera chesapeakensis.
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Figure 1: Phylogenetic tree generated from parsimony analysis based on 28S rDNA sequences. Data were analysed with random addition sequence, and treating gaps as missing data. Bootstrap values ≥ 50 % are shown above or below branches. Thickened branches indicate Bayesian posterior probabilities ≥ 95 %. The tree is rooted with Aniptodera chesapeakensis.

Mentions: The LSU sequence obtained from the herbarium specimen consisted of 724 nucleotides. A BLAST search in NCBI (www.ncbi.nih.gov/blast) showed that this sequence is most similar to Aquaticheirospora lignicola (AY736378, 93 % identity), Munkovalsaria appendiculata (AY772016, 89 %), Leptosphaeria calvescens (AY849944, 89 %) and Montagnula opulenta (AY678086, 89 %). The 28S rDNA data set consisted of 40 sequences. The final data set included 537 characters after alignment. Only part of the Cheirosporium triseriale 28S rDNA sequence was used in the analysis, as most of the included reference sequences from NCBI are shorter than ours. Parsimony analysis resulted in 28 equally parsimonious trees. The KH test showed that these trees were not significantly different. One of these trees is shown in Fig. 1.


Morphological and molecular characterisation of a new anamorphic genus Cheirosporium, from freshwater in China.

Cai L, Guo XY, Hyde KD - Persoonia (2008)

Phylogenetic tree generated from parsimony analysis based on 28S rDNA sequences. Data were analysed with random addition sequence, and treating gaps as missing data. Bootstrap values ≥ 50 % are shown above or below branches. Thickened branches indicate Bayesian posterior probabilities ≥ 95 %. The tree is rooted with Aniptodera chesapeakensis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2865349&req=5

Figure 1: Phylogenetic tree generated from parsimony analysis based on 28S rDNA sequences. Data were analysed with random addition sequence, and treating gaps as missing data. Bootstrap values ≥ 50 % are shown above or below branches. Thickened branches indicate Bayesian posterior probabilities ≥ 95 %. The tree is rooted with Aniptodera chesapeakensis.
Mentions: The LSU sequence obtained from the herbarium specimen consisted of 724 nucleotides. A BLAST search in NCBI (www.ncbi.nih.gov/blast) showed that this sequence is most similar to Aquaticheirospora lignicola (AY736378, 93 % identity), Munkovalsaria appendiculata (AY772016, 89 %), Leptosphaeria calvescens (AY849944, 89 %) and Montagnula opulenta (AY678086, 89 %). The 28S rDNA data set consisted of 40 sequences. The final data set included 537 characters after alignment. Only part of the Cheirosporium triseriale 28S rDNA sequence was used in the analysis, as most of the included reference sequences from NCBI are shorter than ours. Parsimony analysis resulted in 28 equally parsimonious trees. The KH test showed that these trees were not significantly different. One of these trees is shown in Fig. 1.

Bottom Line: The 28S rDNA and ITS rDNA operon of this taxon were amplified and sequenced.Phylogenetic analysis showed that C. triseriale groups with Dictyosporium and allied species, and nests within the Pleosporales (Dothideomycetes, Ascomycota).Cheirosporium is morphologically distinct from the cheirosporous genera Cheiromyces, Cheiromycina, Dictyosporium, Digitomyces, Digitodesmium and Pseudodictyosporium and these differences are discussed.

View Article: PubMed Central - PubMed

Affiliation: Centre for Research in Fungal Diversity, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong;

ABSTRACT
Cheirosporium gen. nov. is characterised by the production of sporodochial conidiomata, semi-macronematous to macronematous conidiophores that possess several distinct sterile branches, and cheiroid, smooth-walled conidia with rhexolytic secession. The 28S rDNA and ITS rDNA operon of this taxon were amplified and sequenced. A BLAST search revealed low homology between Cheirosporium triseriale and existing sequences in public databases, supporting the hypothesis that the species is new to science. Phylogenetic analysis showed that C. triseriale groups with Dictyosporium and allied species, and nests within the Pleosporales (Dothideomycetes, Ascomycota). Cheirosporium is morphologically distinct from the cheirosporous genera Cheiromyces, Cheiromycina, Dictyosporium, Digitomyces, Digitodesmium and Pseudodictyosporium and these differences are discussed.

No MeSH data available.


Related in: MedlinePlus