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Investigation of interactions between poly-L-lysine-coated boron nitride nanotubes and C2C12 cells: up-take, cytocompatibility, and differentiation.

Ciofani G, Ricotti L, Danti S, Moscato S, Nesti C, D'Alessandro D, Dinucci D, Chiellini F, Pietrabissa A, Petrini M, Menciassi A - Int J Nanomedicine (2010)

Bottom Line: In the present in vitro study, we investigated the interactions of poly-l-lysine-coated BNNTs with C2C12 cells, as a model of muscle cells, in terms of cytocompatibility and BNNT internalization.The latter was performed using both confocal and transmission electron microscopy.We demonstrated that BNNTs are highly internalized by C2C12 cells, with neither adversely affecting C2C12 myoblast viability nor significantly interfering with myotube formation.

View Article: PubMed Central - PubMed

Affiliation: Scuola Superiore Sant'Anna, Pisa, Italy.

ABSTRACT
Boron nitride nanotubes (BNNTs) have generated considerable interest within the scientific community by virtue of their unique physical properties, which can be exploited in the biomedical field. In the present in vitro study, we investigated the interactions of poly-l-lysine-coated BNNTs with C2C12 cells, as a model of muscle cells, in terms of cytocompatibility and BNNT internalization. The latter was performed using both confocal and transmission electron microscopy. Finally, we investigated myoblast differentiation in the presence of BNNTs, evaluating the protein synthesis of differentiating cells, myotube formation, and expression of some constitutive myoblastic markers, such as MyoD and Cx43, by reverse transcription - polymerase chain reaction and Western blot analysis. We demonstrated that BNNTs are highly internalized by C2C12 cells, with neither adversely affecting C2C12 myoblast viability nor significantly interfering with myotube formation.

Show MeSH
Confocal analysis of BNNT internalization a) and investigation of its mechanism: BNNT uptake b) and its inhibition after treatment with sodium azide c).
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f2-ijn-5-285: Confocal analysis of BNNT internalization a) and investigation of its mechanism: BNNT uptake b) and its inhibition after treatment with sodium azide c).

Mentions: Figure 2 shows the results of the internalization assays performed with confocal microscopy. Figure 2a depicts consecutive slides of cells (magnification 60X, z-stack step 500 nm) following incubation with BNNTs, showing Qdot-labelled BNNTs (red) strongly internalized in the cell cytoplasm (green). No evidence of BNNTs in the nuclei (blue) of cells was observed.


Investigation of interactions between poly-L-lysine-coated boron nitride nanotubes and C2C12 cells: up-take, cytocompatibility, and differentiation.

Ciofani G, Ricotti L, Danti S, Moscato S, Nesti C, D'Alessandro D, Dinucci D, Chiellini F, Pietrabissa A, Petrini M, Menciassi A - Int J Nanomedicine (2010)

Confocal analysis of BNNT internalization a) and investigation of its mechanism: BNNT uptake b) and its inhibition after treatment with sodium azide c).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2865023&req=5

f2-ijn-5-285: Confocal analysis of BNNT internalization a) and investigation of its mechanism: BNNT uptake b) and its inhibition after treatment with sodium azide c).
Mentions: Figure 2 shows the results of the internalization assays performed with confocal microscopy. Figure 2a depicts consecutive slides of cells (magnification 60X, z-stack step 500 nm) following incubation with BNNTs, showing Qdot-labelled BNNTs (red) strongly internalized in the cell cytoplasm (green). No evidence of BNNTs in the nuclei (blue) of cells was observed.

Bottom Line: In the present in vitro study, we investigated the interactions of poly-l-lysine-coated BNNTs with C2C12 cells, as a model of muscle cells, in terms of cytocompatibility and BNNT internalization.The latter was performed using both confocal and transmission electron microscopy.We demonstrated that BNNTs are highly internalized by C2C12 cells, with neither adversely affecting C2C12 myoblast viability nor significantly interfering with myotube formation.

View Article: PubMed Central - PubMed

Affiliation: Scuola Superiore Sant'Anna, Pisa, Italy.

ABSTRACT
Boron nitride nanotubes (BNNTs) have generated considerable interest within the scientific community by virtue of their unique physical properties, which can be exploited in the biomedical field. In the present in vitro study, we investigated the interactions of poly-l-lysine-coated BNNTs with C2C12 cells, as a model of muscle cells, in terms of cytocompatibility and BNNT internalization. The latter was performed using both confocal and transmission electron microscopy. Finally, we investigated myoblast differentiation in the presence of BNNTs, evaluating the protein synthesis of differentiating cells, myotube formation, and expression of some constitutive myoblastic markers, such as MyoD and Cx43, by reverse transcription - polymerase chain reaction and Western blot analysis. We demonstrated that BNNTs are highly internalized by C2C12 cells, with neither adversely affecting C2C12 myoblast viability nor significantly interfering with myotube formation.

Show MeSH