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Bactericidal effect of iron oxide nanoparticles on Staphylococcus aureus.

Tran N, Mir A, Mallik D, Sinha A, Nayar S, Webster TJ - Int J Nanomedicine (2010)

Bottom Line: In order to study the effects of iron oxide (IO) nanoparticles on Staphylococcus aureus, IO nanoparticles were synthesized via a novel matrix-mediated method using polyvinyl alcohol (PVA).Further, S. aureus were grown in the presence of three different IO nanoparticle concentrations for four, 12, and 24 hours.Live/dead assays were performed and the results provide evidence that IO/PVA nanoparticles inhibited S. aureus growth at the highest concentration (3 mg/mL) at all time points.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, Brown University, Providence, Rhode Island, USA.

ABSTRACT
In order to study the effects of iron oxide (IO) nanoparticles on Staphylococcus aureus, IO nanoparticles were synthesized via a novel matrix-mediated method using polyvinyl alcohol (PVA). The IO nanoparticles were characterized by transmission electron microscopy and dynamic light scattering. Further, S. aureus were grown in the presence of three different IO nanoparticle concentrations for four, 12, and 24 hours. Live/dead assays were performed and the results provide evidence that IO/PVA nanoparticles inhibited S. aureus growth at the highest concentration (3 mg/mL) at all time points.

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Optical density reading of bacteria in IO/PVA solution after four hours, 12 hours, and 24 hours. The results are mean ± SEM. (n = 3). All densities are significantly (*P < 0.01) greater at 12 and 24 hours compared with the four-hour time point.Abbreviations: IO, iron oxide; PVA, polyvinyl alcohol.
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f7-ijn-5-277: Optical density reading of bacteria in IO/PVA solution after four hours, 12 hours, and 24 hours. The results are mean ± SEM. (n = 3). All densities are significantly (*P < 0.01) greater at 12 and 24 hours compared with the four-hour time point.Abbreviations: IO, iron oxide; PVA, polyvinyl alcohol.

Mentions: After four hours of incubation, the optical density measurements (Figure 7) showed that there was no significant difference in bacteria numbers in the presence of IO nanoparticles. However, and more importantly, the results from the live/dead assay demonstrated that after four hours, the ratio of live/dead bacteria was significantly lower in the solution with the highest dose (3 mg/mL) of IO nanoparticles compared with the control sample as well as the low and medium dose samples; the same trend was observed after 12 hours and 24 hours (Figure 8).


Bactericidal effect of iron oxide nanoparticles on Staphylococcus aureus.

Tran N, Mir A, Mallik D, Sinha A, Nayar S, Webster TJ - Int J Nanomedicine (2010)

Optical density reading of bacteria in IO/PVA solution after four hours, 12 hours, and 24 hours. The results are mean ± SEM. (n = 3). All densities are significantly (*P < 0.01) greater at 12 and 24 hours compared with the four-hour time point.Abbreviations: IO, iron oxide; PVA, polyvinyl alcohol.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2865022&req=5

f7-ijn-5-277: Optical density reading of bacteria in IO/PVA solution after four hours, 12 hours, and 24 hours. The results are mean ± SEM. (n = 3). All densities are significantly (*P < 0.01) greater at 12 and 24 hours compared with the four-hour time point.Abbreviations: IO, iron oxide; PVA, polyvinyl alcohol.
Mentions: After four hours of incubation, the optical density measurements (Figure 7) showed that there was no significant difference in bacteria numbers in the presence of IO nanoparticles. However, and more importantly, the results from the live/dead assay demonstrated that after four hours, the ratio of live/dead bacteria was significantly lower in the solution with the highest dose (3 mg/mL) of IO nanoparticles compared with the control sample as well as the low and medium dose samples; the same trend was observed after 12 hours and 24 hours (Figure 8).

Bottom Line: In order to study the effects of iron oxide (IO) nanoparticles on Staphylococcus aureus, IO nanoparticles were synthesized via a novel matrix-mediated method using polyvinyl alcohol (PVA).Further, S. aureus were grown in the presence of three different IO nanoparticle concentrations for four, 12, and 24 hours.Live/dead assays were performed and the results provide evidence that IO/PVA nanoparticles inhibited S. aureus growth at the highest concentration (3 mg/mL) at all time points.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, Brown University, Providence, Rhode Island, USA.

ABSTRACT
In order to study the effects of iron oxide (IO) nanoparticles on Staphylococcus aureus, IO nanoparticles were synthesized via a novel matrix-mediated method using polyvinyl alcohol (PVA). The IO nanoparticles were characterized by transmission electron microscopy and dynamic light scattering. Further, S. aureus were grown in the presence of three different IO nanoparticle concentrations for four, 12, and 24 hours. Live/dead assays were performed and the results provide evidence that IO/PVA nanoparticles inhibited S. aureus growth at the highest concentration (3 mg/mL) at all time points.

Show MeSH
Related in: MedlinePlus