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Ferromagnetic resonance for the quantification of superparamagnetic iron oxide nanoparticles in biological materials.

Gamarra LF, daCosta-Filho AJ, Mamani JB, de Cassia Ruiz R, Pavon LF, Sibov TT, Vieira ED, Silva AC, Pontuschka WM, Amaro E - Int J Nanomedicine (2010)

Bottom Line: The aim of the present work is the presentation of a quantification methodology for the control of the amount of superparamagnetic iron oxide nanoparticles (SPIONs) administered in biological materials by means of the ferromagnetic resonance technique (FMR) applied to studies both in vivo and in vitro.The results were corroborated by X-ray fluorescence.In both studies FMR has proven to be an efficient technique for the SPIONs quantification per volume unit (in vivo) or per labeled cell (in vitro).

View Article: PubMed Central - PubMed

Affiliation: Instituto Israelita de Ensino e Pesquisa Albert Einstein, IIEPAE, São Paulo, Brazil. lgamarra@einstein.br

ABSTRACT
The aim of the present work is the presentation of a quantification methodology for the control of the amount of superparamagnetic iron oxide nanoparticles (SPIONs) administered in biological materials by means of the ferromagnetic resonance technique (FMR) applied to studies both in vivo and in vitro. The in vivo study consisted in the analysis of the elimination and biodistribution kinetics of SPIONs after intravenous administration in Wistar rats. The results were corroborated by X-ray fluorescence. For the in vitro study, a quantitative analysis of the concentration of SPIONs bound to the specific AC133 monoclonal antibodies was carried out in order to detect the expression of the antigenic epitopes (CD133) in stem cells from human umbilical cord blood. In both studies FMR has proven to be an efficient technique for the SPIONs quantification per volume unit (in vivo) or per labeled cell (in vitro).

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A) TEM of the control stem cells CD133−. B, C) TEM of the CD133+ stem cells labeled with SPIONs. D) TEM of the CD133+ cells of not conserved morphology. n = nucleus, c = cytoplasm, arrow 1 = electrondense granules on the cell surface, arrow 2 = electrondense granules in the cell cytoplasm. Scale: A) 1.0 μm; B, C) 0.25 μm; D) 0.5 μm.Abbreviations: SPIONs, superparamagnetic iron oxide nanoparticles; TEM, transmission electron microscopy.
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f4-ijn-5-203: A) TEM of the control stem cells CD133−. B, C) TEM of the CD133+ stem cells labeled with SPIONs. D) TEM of the CD133+ cells of not conserved morphology. n = nucleus, c = cytoplasm, arrow 1 = electrondense granules on the cell surface, arrow 2 = electrondense granules in the cell cytoplasm. Scale: A) 1.0 μm; B, C) 0.25 μm; D) 0.5 μm.Abbreviations: SPIONs, superparamagnetic iron oxide nanoparticles; TEM, transmission electron microscopy.

Mentions: The SPIONs concentrations were determined from the areas under the FMR absorption spectra by interpolation in the calibration curve shown in Figure 1. The calculation was performed with the FMR data from samples obtained after the time intervals discussed in the protocol and also from the control sample (see Figures 4 and 6 in Gamarra and colleagues25). The experimental points represent the average values obtained from FMR measurements of three samples for each condition under investigation.


Ferromagnetic resonance for the quantification of superparamagnetic iron oxide nanoparticles in biological materials.

Gamarra LF, daCosta-Filho AJ, Mamani JB, de Cassia Ruiz R, Pavon LF, Sibov TT, Vieira ED, Silva AC, Pontuschka WM, Amaro E - Int J Nanomedicine (2010)

A) TEM of the control stem cells CD133−. B, C) TEM of the CD133+ stem cells labeled with SPIONs. D) TEM of the CD133+ cells of not conserved morphology. n = nucleus, c = cytoplasm, arrow 1 = electrondense granules on the cell surface, arrow 2 = electrondense granules in the cell cytoplasm. Scale: A) 1.0 μm; B, C) 0.25 μm; D) 0.5 μm.Abbreviations: SPIONs, superparamagnetic iron oxide nanoparticles; TEM, transmission electron microscopy.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2865015&req=5

f4-ijn-5-203: A) TEM of the control stem cells CD133−. B, C) TEM of the CD133+ stem cells labeled with SPIONs. D) TEM of the CD133+ cells of not conserved morphology. n = nucleus, c = cytoplasm, arrow 1 = electrondense granules on the cell surface, arrow 2 = electrondense granules in the cell cytoplasm. Scale: A) 1.0 μm; B, C) 0.25 μm; D) 0.5 μm.Abbreviations: SPIONs, superparamagnetic iron oxide nanoparticles; TEM, transmission electron microscopy.
Mentions: The SPIONs concentrations were determined from the areas under the FMR absorption spectra by interpolation in the calibration curve shown in Figure 1. The calculation was performed with the FMR data from samples obtained after the time intervals discussed in the protocol and also from the control sample (see Figures 4 and 6 in Gamarra and colleagues25). The experimental points represent the average values obtained from FMR measurements of three samples for each condition under investigation.

Bottom Line: The aim of the present work is the presentation of a quantification methodology for the control of the amount of superparamagnetic iron oxide nanoparticles (SPIONs) administered in biological materials by means of the ferromagnetic resonance technique (FMR) applied to studies both in vivo and in vitro.The results were corroborated by X-ray fluorescence.In both studies FMR has proven to be an efficient technique for the SPIONs quantification per volume unit (in vivo) or per labeled cell (in vitro).

View Article: PubMed Central - PubMed

Affiliation: Instituto Israelita de Ensino e Pesquisa Albert Einstein, IIEPAE, São Paulo, Brazil. lgamarra@einstein.br

ABSTRACT
The aim of the present work is the presentation of a quantification methodology for the control of the amount of superparamagnetic iron oxide nanoparticles (SPIONs) administered in biological materials by means of the ferromagnetic resonance technique (FMR) applied to studies both in vivo and in vitro. The in vivo study consisted in the analysis of the elimination and biodistribution kinetics of SPIONs after intravenous administration in Wistar rats. The results were corroborated by X-ray fluorescence. For the in vitro study, a quantitative analysis of the concentration of SPIONs bound to the specific AC133 monoclonal antibodies was carried out in order to detect the expression of the antigenic epitopes (CD133) in stem cells from human umbilical cord blood. In both studies FMR has proven to be an efficient technique for the SPIONs quantification per volume unit (in vivo) or per labeled cell (in vitro).

Show MeSH
Related in: MedlinePlus