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Ferromagnetic resonance for the quantification of superparamagnetic iron oxide nanoparticles in biological materials.

Gamarra LF, daCosta-Filho AJ, Mamani JB, de Cassia Ruiz R, Pavon LF, Sibov TT, Vieira ED, Silva AC, Pontuschka WM, Amaro E - Int J Nanomedicine (2010)

Bottom Line: The aim of the present work is the presentation of a quantification methodology for the control of the amount of superparamagnetic iron oxide nanoparticles (SPIONs) administered in biological materials by means of the ferromagnetic resonance technique (FMR) applied to studies both in vivo and in vitro.The results were corroborated by X-ray fluorescence.In both studies FMR has proven to be an efficient technique for the SPIONs quantification per volume unit (in vivo) or per labeled cell (in vitro).

View Article: PubMed Central - PubMed

Affiliation: Instituto Israelita de Ensino e Pesquisa Albert Einstein, IIEPAE, São Paulo, Brazil. lgamarra@einstein.br

ABSTRACT
The aim of the present work is the presentation of a quantification methodology for the control of the amount of superparamagnetic iron oxide nanoparticles (SPIONs) administered in biological materials by means of the ferromagnetic resonance technique (FMR) applied to studies both in vivo and in vitro. The in vivo study consisted in the analysis of the elimination and biodistribution kinetics of SPIONs after intravenous administration in Wistar rats. The results were corroborated by X-ray fluorescence. For the in vitro study, a quantitative analysis of the concentration of SPIONs bound to the specific AC133 monoclonal antibodies was carried out in order to detect the expression of the antigenic epitopes (CD133) in stem cells from human umbilical cord blood. In both studies FMR has proven to be an efficient technique for the SPIONs quantification per volume unit (in vivo) or per labeled cell (in vitro).

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FMR (solid line) and XRF (dashed line) calibration curves of the SPIONs related to the area under the resonance line at g = 2.1 and the characteristic FeKa line, respectively. In the inset A) there are seen typical FMR spectra for different concentrations of iron, corresponding to the characteristic line of magnetite contained in the ferrofluid Endorem™. Inset B) shows the characteristic FeKa line of XRF, proportional to the iron concentration in the sample.Abbreviations: FMR, ferromagnetic resonance; SPIONs, superparamagnetic iron oxide nanoparticles; XRF, X-ray fluorescence.
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f1-ijn-5-203: FMR (solid line) and XRF (dashed line) calibration curves of the SPIONs related to the area under the resonance line at g = 2.1 and the characteristic FeKa line, respectively. In the inset A) there are seen typical FMR spectra for different concentrations of iron, corresponding to the characteristic line of magnetite contained in the ferrofluid Endorem™. Inset B) shows the characteristic FeKa line of XRF, proportional to the iron concentration in the sample.Abbreviations: FMR, ferromagnetic resonance; SPIONs, superparamagnetic iron oxide nanoparticles; XRF, X-ray fluorescence.

Mentions: The calibration curve shown in Figure 1 was constructed in order to determine the average number of SPIONs per volume unit of the samples being studied. Samples of the ferrofluid (Endorem) were prepared with colloidal suspensions at different concentrations varying from 0.3 to 101 × 1013 SPION/mm3. A 2 μL volume of each sample was inserted into capillary quartz tubes for the acquisition of the FMR spectra as shown in Figure 1A. Each point in the calibration curve was the result of three scans and the plotted value represents the average of these measurements.


Ferromagnetic resonance for the quantification of superparamagnetic iron oxide nanoparticles in biological materials.

Gamarra LF, daCosta-Filho AJ, Mamani JB, de Cassia Ruiz R, Pavon LF, Sibov TT, Vieira ED, Silva AC, Pontuschka WM, Amaro E - Int J Nanomedicine (2010)

FMR (solid line) and XRF (dashed line) calibration curves of the SPIONs related to the area under the resonance line at g = 2.1 and the characteristic FeKa line, respectively. In the inset A) there are seen typical FMR spectra for different concentrations of iron, corresponding to the characteristic line of magnetite contained in the ferrofluid Endorem™. Inset B) shows the characteristic FeKa line of XRF, proportional to the iron concentration in the sample.Abbreviations: FMR, ferromagnetic resonance; SPIONs, superparamagnetic iron oxide nanoparticles; XRF, X-ray fluorescence.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2865015&req=5

f1-ijn-5-203: FMR (solid line) and XRF (dashed line) calibration curves of the SPIONs related to the area under the resonance line at g = 2.1 and the characteristic FeKa line, respectively. In the inset A) there are seen typical FMR spectra for different concentrations of iron, corresponding to the characteristic line of magnetite contained in the ferrofluid Endorem™. Inset B) shows the characteristic FeKa line of XRF, proportional to the iron concentration in the sample.Abbreviations: FMR, ferromagnetic resonance; SPIONs, superparamagnetic iron oxide nanoparticles; XRF, X-ray fluorescence.
Mentions: The calibration curve shown in Figure 1 was constructed in order to determine the average number of SPIONs per volume unit of the samples being studied. Samples of the ferrofluid (Endorem) were prepared with colloidal suspensions at different concentrations varying from 0.3 to 101 × 1013 SPION/mm3. A 2 μL volume of each sample was inserted into capillary quartz tubes for the acquisition of the FMR spectra as shown in Figure 1A. Each point in the calibration curve was the result of three scans and the plotted value represents the average of these measurements.

Bottom Line: The aim of the present work is the presentation of a quantification methodology for the control of the amount of superparamagnetic iron oxide nanoparticles (SPIONs) administered in biological materials by means of the ferromagnetic resonance technique (FMR) applied to studies both in vivo and in vitro.The results were corroborated by X-ray fluorescence.In both studies FMR has proven to be an efficient technique for the SPIONs quantification per volume unit (in vivo) or per labeled cell (in vitro).

View Article: PubMed Central - PubMed

Affiliation: Instituto Israelita de Ensino e Pesquisa Albert Einstein, IIEPAE, São Paulo, Brazil. lgamarra@einstein.br

ABSTRACT
The aim of the present work is the presentation of a quantification methodology for the control of the amount of superparamagnetic iron oxide nanoparticles (SPIONs) administered in biological materials by means of the ferromagnetic resonance technique (FMR) applied to studies both in vivo and in vitro. The in vivo study consisted in the analysis of the elimination and biodistribution kinetics of SPIONs after intravenous administration in Wistar rats. The results were corroborated by X-ray fluorescence. For the in vitro study, a quantitative analysis of the concentration of SPIONs bound to the specific AC133 monoclonal antibodies was carried out in order to detect the expression of the antigenic epitopes (CD133) in stem cells from human umbilical cord blood. In both studies FMR has proven to be an efficient technique for the SPIONs quantification per volume unit (in vivo) or per labeled cell (in vitro).

Show MeSH
Related in: MedlinePlus