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Bactericidal effects of silver plus titanium dioxide-coated endotracheal tubes on Pseudomonas aeruginosa and Staphylococcus aureus.

Tarquinio KM, Kothurkar NK, Goswami DY, Sanders RC, Zaritsky AL, LeVine AM - Int J Nanomedicine (2010)

Bottom Line: Compared to standard ETTs, P. aeruginosa growth was significantly inhibited by solgel TiO(2) with Ag at 24 hours, and by Degussa TiO(2) with Ag at 24 and 48 hours after inoculation.In vitro, solgel TiO(2) with Ag and Degussa TiO(2) with Ag both attenuated P. aeruginosa growth, but demonstrated no effect on S. aureus colonization.Further studies using alternative coating and incorporating UV light exposure are needed to identify their potential utility in reducing VAP.

View Article: PubMed Central - PubMed

Affiliation: Division of Pediatric Critical Care Medicine, Department of Pediatrics, Rhode Island Hospital, The Warren Alpert Medical School, Brown University, Providence, RI, USA. ktarquinio@lifespan.org

ABSTRACT

Purpose: Ventilator-associated pneumonia (VAP) is a nosocomial infection resulting in significant morbidity and mortality. Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) are pathogens associated with VAP. Silver (Ag) coating of endotracheal tubes (ETTs) reduces bacterial colonization, however titanium dioxide (TiO(2)) coating has not been studied.

Methods: Five types of ETT coatings were applied over silica layer: Ag, solgel TiO(2), solgel TiO(2) with Ag, Degussa P25 TiO(2) (Degussa TiO(2)), and Degussa TiO(2) with Ag. After ETTs were incubated with P. aeruginosa or S. aureus; colonization was determined quantitatively.

Results: Pseudomonas aeruginosa and S. aureus grew for 5 days on standard ETTs. Compared to standard ETTs, P. aeruginosa growth was significantly inhibited by solgel TiO(2) with Ag at 24 hours, and by Degussa TiO(2) with Ag at 24 and 48 hours after inoculation. No significant difference in S. aureus growth was observed between the control and any of the five coatings for 5 days.

Conclusion: In vitro, solgel TiO(2) with Ag and Degussa TiO(2) with Ag both attenuated P. aeruginosa growth, but demonstrated no effect on S. aureus colonization. Further studies using alternative coating and incorporating UV light exposure are needed to identify their potential utility in reducing VAP.

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Related in: MedlinePlus

Characterization of S. aureus growth on polyvinyl chloride endotracheal tubes coated with titanium dioxide with silver over a 144-hour period. Comparison of standard, solgel titanium dioxide with silver and Degussa titanium dioxide with silver endotracheal tubes. There was no difference in S. aureus growth in the titanium dioxide with silver-coated groups compared to standard endotracheal tubes except at 144 hours with solgel titanium dioxide with silver endotracheal tubes.Note: N = 4.Abbreviation: CFU, colony-forming units.
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f7-ijn-5-177: Characterization of S. aureus growth on polyvinyl chloride endotracheal tubes coated with titanium dioxide with silver over a 144-hour period. Comparison of standard, solgel titanium dioxide with silver and Degussa titanium dioxide with silver endotracheal tubes. There was no difference in S. aureus growth in the titanium dioxide with silver-coated groups compared to standard endotracheal tubes except at 144 hours with solgel titanium dioxide with silver endotracheal tubes.Note: N = 4.Abbreviation: CFU, colony-forming units.

Mentions: Growth was observed up to 144 hours. Staphylococcus aureus growth peaked at 48 hours after inoculation on both standard and Ag-coated ETTs without a difference over 144 hours (Figure 5). Furthermore, there was also no difference in S. aureus growth over 144 hours between standard, solgel TiO2 and Degussa TiO2-coated ETTs (Figure 6). Compared to standard ETTs, neither solgel TiO2 with Ag nor Degussa TiO2 with Ag showed a difference in S. aureus growth over a 144 hour period except solgel TiO2 with Ag at the 144 hour point after inoculation in which there was a significant attenuation of S. aureus growth compared to the standard ETT (Figure 7).


Bactericidal effects of silver plus titanium dioxide-coated endotracheal tubes on Pseudomonas aeruginosa and Staphylococcus aureus.

Tarquinio KM, Kothurkar NK, Goswami DY, Sanders RC, Zaritsky AL, LeVine AM - Int J Nanomedicine (2010)

Characterization of S. aureus growth on polyvinyl chloride endotracheal tubes coated with titanium dioxide with silver over a 144-hour period. Comparison of standard, solgel titanium dioxide with silver and Degussa titanium dioxide with silver endotracheal tubes. There was no difference in S. aureus growth in the titanium dioxide with silver-coated groups compared to standard endotracheal tubes except at 144 hours with solgel titanium dioxide with silver endotracheal tubes.Note: N = 4.Abbreviation: CFU, colony-forming units.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2865012&req=5

f7-ijn-5-177: Characterization of S. aureus growth on polyvinyl chloride endotracheal tubes coated with titanium dioxide with silver over a 144-hour period. Comparison of standard, solgel titanium dioxide with silver and Degussa titanium dioxide with silver endotracheal tubes. There was no difference in S. aureus growth in the titanium dioxide with silver-coated groups compared to standard endotracheal tubes except at 144 hours with solgel titanium dioxide with silver endotracheal tubes.Note: N = 4.Abbreviation: CFU, colony-forming units.
Mentions: Growth was observed up to 144 hours. Staphylococcus aureus growth peaked at 48 hours after inoculation on both standard and Ag-coated ETTs without a difference over 144 hours (Figure 5). Furthermore, there was also no difference in S. aureus growth over 144 hours between standard, solgel TiO2 and Degussa TiO2-coated ETTs (Figure 6). Compared to standard ETTs, neither solgel TiO2 with Ag nor Degussa TiO2 with Ag showed a difference in S. aureus growth over a 144 hour period except solgel TiO2 with Ag at the 144 hour point after inoculation in which there was a significant attenuation of S. aureus growth compared to the standard ETT (Figure 7).

Bottom Line: Compared to standard ETTs, P. aeruginosa growth was significantly inhibited by solgel TiO(2) with Ag at 24 hours, and by Degussa TiO(2) with Ag at 24 and 48 hours after inoculation.In vitro, solgel TiO(2) with Ag and Degussa TiO(2) with Ag both attenuated P. aeruginosa growth, but demonstrated no effect on S. aureus colonization.Further studies using alternative coating and incorporating UV light exposure are needed to identify their potential utility in reducing VAP.

View Article: PubMed Central - PubMed

Affiliation: Division of Pediatric Critical Care Medicine, Department of Pediatrics, Rhode Island Hospital, The Warren Alpert Medical School, Brown University, Providence, RI, USA. ktarquinio@lifespan.org

ABSTRACT

Purpose: Ventilator-associated pneumonia (VAP) is a nosocomial infection resulting in significant morbidity and mortality. Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) are pathogens associated with VAP. Silver (Ag) coating of endotracheal tubes (ETTs) reduces bacterial colonization, however titanium dioxide (TiO(2)) coating has not been studied.

Methods: Five types of ETT coatings were applied over silica layer: Ag, solgel TiO(2), solgel TiO(2) with Ag, Degussa P25 TiO(2) (Degussa TiO(2)), and Degussa TiO(2) with Ag. After ETTs were incubated with P. aeruginosa or S. aureus; colonization was determined quantitatively.

Results: Pseudomonas aeruginosa and S. aureus grew for 5 days on standard ETTs. Compared to standard ETTs, P. aeruginosa growth was significantly inhibited by solgel TiO(2) with Ag at 24 hours, and by Degussa TiO(2) with Ag at 24 and 48 hours after inoculation. No significant difference in S. aureus growth was observed between the control and any of the five coatings for 5 days.

Conclusion: In vitro, solgel TiO(2) with Ag and Degussa TiO(2) with Ag both attenuated P. aeruginosa growth, but demonstrated no effect on S. aureus colonization. Further studies using alternative coating and incorporating UV light exposure are needed to identify their potential utility in reducing VAP.

Show MeSH
Related in: MedlinePlus