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Bactericidal effects of silver plus titanium dioxide-coated endotracheal tubes on Pseudomonas aeruginosa and Staphylococcus aureus.

Tarquinio KM, Kothurkar NK, Goswami DY, Sanders RC, Zaritsky AL, LeVine AM - Int J Nanomedicine (2010)

Bottom Line: Compared to standard ETTs, P. aeruginosa growth was significantly inhibited by solgel TiO(2) with Ag at 24 hours, and by Degussa TiO(2) with Ag at 24 and 48 hours after inoculation.In vitro, solgel TiO(2) with Ag and Degussa TiO(2) with Ag both attenuated P. aeruginosa growth, but demonstrated no effect on S. aureus colonization.Further studies using alternative coating and incorporating UV light exposure are needed to identify their potential utility in reducing VAP.

View Article: PubMed Central - PubMed

Affiliation: Division of Pediatric Critical Care Medicine, Department of Pediatrics, Rhode Island Hospital, The Warren Alpert Medical School, Brown University, Providence, RI, USA. ktarquinio@lifespan.org

ABSTRACT

Purpose: Ventilator-associated pneumonia (VAP) is a nosocomial infection resulting in significant morbidity and mortality. Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) are pathogens associated with VAP. Silver (Ag) coating of endotracheal tubes (ETTs) reduces bacterial colonization, however titanium dioxide (TiO(2)) coating has not been studied.

Methods: Five types of ETT coatings were applied over silica layer: Ag, solgel TiO(2), solgel TiO(2) with Ag, Degussa P25 TiO(2) (Degussa TiO(2)), and Degussa TiO(2) with Ag. After ETTs were incubated with P. aeruginosa or S. aureus; colonization was determined quantitatively.

Results: Pseudomonas aeruginosa and S. aureus grew for 5 days on standard ETTs. Compared to standard ETTs, P. aeruginosa growth was significantly inhibited by solgel TiO(2) with Ag at 24 hours, and by Degussa TiO(2) with Ag at 24 and 48 hours after inoculation. No significant difference in S. aureus growth was observed between the control and any of the five coatings for 5 days.

Conclusion: In vitro, solgel TiO(2) with Ag and Degussa TiO(2) with Ag both attenuated P. aeruginosa growth, but demonstrated no effect on S. aureus colonization. Further studies using alternative coating and incorporating UV light exposure are needed to identify their potential utility in reducing VAP.

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Related in: MedlinePlus

Standard endotracheal tubes were coated with a passivating layer of colloidal silica and served as controls. Five types of coatings were applied over a silica layer; 1. solgel titanium dioxide without silver, 2. solgel titanium dioxide with silver, 3. Degussa titanium dioxide without silver, 4. Degussa titanium dioxide with silver and 5. silver only (not shown).
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f1-ijn-5-177: Standard endotracheal tubes were coated with a passivating layer of colloidal silica and served as controls. Five types of coatings were applied over a silica layer; 1. solgel titanium dioxide without silver, 2. solgel titanium dioxide with silver, 3. Degussa titanium dioxide without silver, 4. Degussa titanium dioxide with silver and 5. silver only (not shown).

Mentions: The respective TiO2 and Ag treatments are summarized in Table 1. Five types of coatings were applied over the SiO2 layer (Figure 1): 1) solgel TiO2, 2) solgel TiO2 with Ag, 3) Degussa TiO2 (Evonik Degussa, Parsippany, NJ, USA), 4) Degussa TiO2 with Ag, and 5) Ag (not shown in Figure 1). Each of the coating solutions A–D had 0.19 wt% TiO2 on a dry weight basis. Water and ethanol were used as solvents in variable ratios for each solution. Solgel TiO2 was used for solutions A and B, while C and D solutions consisted of solgel and Degussa TiO2 in the ratio 20:80 wt/wt. Silver nitrate (AgNO3) was added to solutions B and D. For solution E, AgNO3 0.1 wt% was mixed in ethanol-water (50:50 wt/wt). Two types of TiO2 coatings were applied; solgel to increase adherence to PVC for substrate depositition and Degussa TiO2 for potentially high photocatalytic performance. Since Degussa TiO2 suspensions had very poor adhesion to the ETTs, they were mixed with a small amount of sols of solgel TiO2. After TiO2 coating, the ETTs were dried with 85°C air for 1 minute, followed by further drying in an air-circulating oven at 100°C for 15 minutes, then packed while warm.


Bactericidal effects of silver plus titanium dioxide-coated endotracheal tubes on Pseudomonas aeruginosa and Staphylococcus aureus.

Tarquinio KM, Kothurkar NK, Goswami DY, Sanders RC, Zaritsky AL, LeVine AM - Int J Nanomedicine (2010)

Standard endotracheal tubes were coated with a passivating layer of colloidal silica and served as controls. Five types of coatings were applied over a silica layer; 1. solgel titanium dioxide without silver, 2. solgel titanium dioxide with silver, 3. Degussa titanium dioxide without silver, 4. Degussa titanium dioxide with silver and 5. silver only (not shown).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2865012&req=5

f1-ijn-5-177: Standard endotracheal tubes were coated with a passivating layer of colloidal silica and served as controls. Five types of coatings were applied over a silica layer; 1. solgel titanium dioxide without silver, 2. solgel titanium dioxide with silver, 3. Degussa titanium dioxide without silver, 4. Degussa titanium dioxide with silver and 5. silver only (not shown).
Mentions: The respective TiO2 and Ag treatments are summarized in Table 1. Five types of coatings were applied over the SiO2 layer (Figure 1): 1) solgel TiO2, 2) solgel TiO2 with Ag, 3) Degussa TiO2 (Evonik Degussa, Parsippany, NJ, USA), 4) Degussa TiO2 with Ag, and 5) Ag (not shown in Figure 1). Each of the coating solutions A–D had 0.19 wt% TiO2 on a dry weight basis. Water and ethanol were used as solvents in variable ratios for each solution. Solgel TiO2 was used for solutions A and B, while C and D solutions consisted of solgel and Degussa TiO2 in the ratio 20:80 wt/wt. Silver nitrate (AgNO3) was added to solutions B and D. For solution E, AgNO3 0.1 wt% was mixed in ethanol-water (50:50 wt/wt). Two types of TiO2 coatings were applied; solgel to increase adherence to PVC for substrate depositition and Degussa TiO2 for potentially high photocatalytic performance. Since Degussa TiO2 suspensions had very poor adhesion to the ETTs, they were mixed with a small amount of sols of solgel TiO2. After TiO2 coating, the ETTs were dried with 85°C air for 1 minute, followed by further drying in an air-circulating oven at 100°C for 15 minutes, then packed while warm.

Bottom Line: Compared to standard ETTs, P. aeruginosa growth was significantly inhibited by solgel TiO(2) with Ag at 24 hours, and by Degussa TiO(2) with Ag at 24 and 48 hours after inoculation.In vitro, solgel TiO(2) with Ag and Degussa TiO(2) with Ag both attenuated P. aeruginosa growth, but demonstrated no effect on S. aureus colonization.Further studies using alternative coating and incorporating UV light exposure are needed to identify their potential utility in reducing VAP.

View Article: PubMed Central - PubMed

Affiliation: Division of Pediatric Critical Care Medicine, Department of Pediatrics, Rhode Island Hospital, The Warren Alpert Medical School, Brown University, Providence, RI, USA. ktarquinio@lifespan.org

ABSTRACT

Purpose: Ventilator-associated pneumonia (VAP) is a nosocomial infection resulting in significant morbidity and mortality. Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) are pathogens associated with VAP. Silver (Ag) coating of endotracheal tubes (ETTs) reduces bacterial colonization, however titanium dioxide (TiO(2)) coating has not been studied.

Methods: Five types of ETT coatings were applied over silica layer: Ag, solgel TiO(2), solgel TiO(2) with Ag, Degussa P25 TiO(2) (Degussa TiO(2)), and Degussa TiO(2) with Ag. After ETTs were incubated with P. aeruginosa or S. aureus; colonization was determined quantitatively.

Results: Pseudomonas aeruginosa and S. aureus grew for 5 days on standard ETTs. Compared to standard ETTs, P. aeruginosa growth was significantly inhibited by solgel TiO(2) with Ag at 24 hours, and by Degussa TiO(2) with Ag at 24 and 48 hours after inoculation. No significant difference in S. aureus growth was observed between the control and any of the five coatings for 5 days.

Conclusion: In vitro, solgel TiO(2) with Ag and Degussa TiO(2) with Ag both attenuated P. aeruginosa growth, but demonstrated no effect on S. aureus colonization. Further studies using alternative coating and incorporating UV light exposure are needed to identify their potential utility in reducing VAP.

Show MeSH
Related in: MedlinePlus