Limits...
Carbon-covered magnetic nanomaterials and their application for the thermolysis of cancer cells.

Xu Y, Mahmood M, Fejleh A, Li Z, Watanabe F, Trigwell S, Little RB, Kunets VP, Dervishi E, Biris AR, Salamo GJ, Biris AS - Int J Nanomedicine (2010)

Bottom Line: X-ray diffraction and X-ray photoelectron spectroscopy analysis revealed that the cores inside the carbon shells of these NPs were preserved in their metallic states.Low RF radiation of 350 kHz induced localized heating of the magnetic NPs, which triggered cell death.Apoptosis inducement was found to be dependent on the RF irradiation time and NP concentration.

View Article: PubMed Central - PubMed

Affiliation: Nanotechnology Center and Applied Science Department, University of Arkansas at Little Rock, Little Rock, AR, USA. yxxu@ualr.edu; asbiris@ualr.edu

ABSTRACT
Three types of graphitic shelled-magnetic core (Fe, Fe/Co, and Co) nanoparticles (named as C-Fe, C-Fe/Co, and C-Co NPs) were synthesized by radio frequency-catalytic chemical vapor deposition (RF-cCVD). X-ray diffraction and X-ray photoelectron spectroscopy analysis revealed that the cores inside the carbon shells of these NPs were preserved in their metallic states. Fluorescence microscopy images indicated effective penetrations of the NPs through the cellular membranes of cultured cancer HeLa cells, both inside the cytoplasm and the nucleus. Low RF radiation of 350 kHz induced localized heating of the magnetic NPs, which triggered cell death. Apoptosis inducement was found to be dependent on the RF irradiation time and NP concentration. It was showed that the Fe-C NPs had a much higher ability of killing the cancer cells (over 99%) compared with the other types of NPs (C-Co or C-Fe/Co), even at a very low concentration of 0.83 microg/mL. The localized heating of NPs inside the cancer cells comes from the hysteresis heating and resistive heating through eddy currents generated under the RF radiation. The RF thermal ablation properties of the magnetic NPs were correlated with the analysis provided by a superconducting quantum interference device (SQUID).

Show MeSH

Related in: MedlinePlus

A) AFM topographic images showing individual C-Co NPs. B) Low and C, D, E) high magnification TEM images of graphitic C) C-Co, D) C-Fe and E) C-Fe/Co NPs obtained by RF-cCVD method.Abbreviations:
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2865011&req=5

f1-ijn-5-167: A) AFM topographic images showing individual C-Co NPs. B) Low and C, D, E) high magnification TEM images of graphitic C) C-Co, D) C-Fe and E) C-Fe/Co NPs obtained by RF-cCVD method.Abbreviations:

Mentions: The magnetic NPs were synthesized by a RF-cCVD process.10 AFM and TEM analysis revealed that the average size of the C-Fe, C-Fe/Co and C-Co NPs was 8 ± 1.0 nm, 9 ± 1.5 nm and 7 ± 1.2 nm, respectively, with the mean and standard deviation of sizes measured by TEM for ∼100 NPs in each samples. The NPs were covered with 5–8 layers, 7–10 layers, and 2–4 layers of graphitic carbon respectively, as shown in Figure 1.


Carbon-covered magnetic nanomaterials and their application for the thermolysis of cancer cells.

Xu Y, Mahmood M, Fejleh A, Li Z, Watanabe F, Trigwell S, Little RB, Kunets VP, Dervishi E, Biris AR, Salamo GJ, Biris AS - Int J Nanomedicine (2010)

A) AFM topographic images showing individual C-Co NPs. B) Low and C, D, E) high magnification TEM images of graphitic C) C-Co, D) C-Fe and E) C-Fe/Co NPs obtained by RF-cCVD method.Abbreviations:
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2865011&req=5

f1-ijn-5-167: A) AFM topographic images showing individual C-Co NPs. B) Low and C, D, E) high magnification TEM images of graphitic C) C-Co, D) C-Fe and E) C-Fe/Co NPs obtained by RF-cCVD method.Abbreviations:
Mentions: The magnetic NPs were synthesized by a RF-cCVD process.10 AFM and TEM analysis revealed that the average size of the C-Fe, C-Fe/Co and C-Co NPs was 8 ± 1.0 nm, 9 ± 1.5 nm and 7 ± 1.2 nm, respectively, with the mean and standard deviation of sizes measured by TEM for ∼100 NPs in each samples. The NPs were covered with 5–8 layers, 7–10 layers, and 2–4 layers of graphitic carbon respectively, as shown in Figure 1.

Bottom Line: X-ray diffraction and X-ray photoelectron spectroscopy analysis revealed that the cores inside the carbon shells of these NPs were preserved in their metallic states.Low RF radiation of 350 kHz induced localized heating of the magnetic NPs, which triggered cell death.Apoptosis inducement was found to be dependent on the RF irradiation time and NP concentration.

View Article: PubMed Central - PubMed

Affiliation: Nanotechnology Center and Applied Science Department, University of Arkansas at Little Rock, Little Rock, AR, USA. yxxu@ualr.edu; asbiris@ualr.edu

ABSTRACT
Three types of graphitic shelled-magnetic core (Fe, Fe/Co, and Co) nanoparticles (named as C-Fe, C-Fe/Co, and C-Co NPs) were synthesized by radio frequency-catalytic chemical vapor deposition (RF-cCVD). X-ray diffraction and X-ray photoelectron spectroscopy analysis revealed that the cores inside the carbon shells of these NPs were preserved in their metallic states. Fluorescence microscopy images indicated effective penetrations of the NPs through the cellular membranes of cultured cancer HeLa cells, both inside the cytoplasm and the nucleus. Low RF radiation of 350 kHz induced localized heating of the magnetic NPs, which triggered cell death. Apoptosis inducement was found to be dependent on the RF irradiation time and NP concentration. It was showed that the Fe-C NPs had a much higher ability of killing the cancer cells (over 99%) compared with the other types of NPs (C-Co or C-Fe/Co), even at a very low concentration of 0.83 microg/mL. The localized heating of NPs inside the cancer cells comes from the hysteresis heating and resistive heating through eddy currents generated under the RF radiation. The RF thermal ablation properties of the magnetic NPs were correlated with the analysis provided by a superconducting quantum interference device (SQUID).

Show MeSH
Related in: MedlinePlus