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Expressions of neuregulin 1beta and ErbB4 in prefrontal cortex and hippocampus of a rat schizophrenia model induced by chronic MK-801 administration.

Feng Y, Wang XD, Guo CM, Yang Y, Li JT, Su YA, Si TM - J. Biomed. Biotechnol. (2010)

Bottom Line: Our data showed that NRG1beta and ErbB4 expressions were significantly increased in the rat prefrontal cortex and hippocampus but in different subregions.These findings suggest that altered expressions of NRG1 and ErbB4 might be attributed to the schizophrenia.Further study in the role and mechanism of NRG1 and ErbB4 may lead to better understanding of the pathophysiology for this disorder.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Mental Health of National Health Ministry, Institute of Mental Health, Peking University, No. 51 Hua Yuan Bei Road, Haidian District, Beijing 100191, China.

ABSTRACT
Recent human genetic studies and postmortem brain examinations of schizophrenia patients strongly indicate that dysregulation of NRG1 and ErbB4 may be important pathogenic factors of schizophrenia. However, this hypothesis has not been validated and fully investigated in animal models of schizophrenia. In this study we quantitatively examined NRG1 and ErbB4 protein expressions by immunohistochemistry and Western blot in the brain of a rat schizophrenia model induced by chronic administration of MK-801 (a noncompetitive NMDA receptor antagonist). Our data showed that NRG1beta and ErbB4 expressions were significantly increased in the rat prefrontal cortex and hippocampus but in different subregions. These findings suggest that altered expressions of NRG1 and ErbB4 might be attributed to the schizophrenia. Further study in the role and mechanism of NRG1 and ErbB4 may lead to better understanding of the pathophysiology for this disorder.

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Expression of NRG1β and ErbB4 proteins in the rat prefrontal cortex. (a) Representative Western blots of NRG1β protein isoforms. (b) Quantitative analysis of NRG1β immunoblots. (c) Representative Western blots of ErbB4 proteins isoforms. (d) Quantitative analysis of ErbB4 immunoblots. The data were normalized by taking the value of vehicle group as 100% and expressed as means ± S.E.M. (n = 5). *P < .05. (e) Representative section of ErbB4 immunohistochemistry in the PrL region of prefrontal cortex from vehicle group. (f) Representative section of ErbB4 immunohistochemistry in the PrL region of prefrontal cortex from MK-801-treated group. (g) Representative section of ErbB4 immunohistochemistry in the Cg1 and M2 region of prefrontal cortex from vehicle group. (h) Representative section of ErbB4 immunohistochemistry in the Cg1 and M2 region of prefrontal cortex from MK-801-treated group. Arrows indicate ErbB4-expressing neurons in the prefrontal cortex. Scale bar for (e)–(h): 50 μm.
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fig1: Expression of NRG1β and ErbB4 proteins in the rat prefrontal cortex. (a) Representative Western blots of NRG1β protein isoforms. (b) Quantitative analysis of NRG1β immunoblots. (c) Representative Western blots of ErbB4 proteins isoforms. (d) Quantitative analysis of ErbB4 immunoblots. The data were normalized by taking the value of vehicle group as 100% and expressed as means ± S.E.M. (n = 5). *P < .05. (e) Representative section of ErbB4 immunohistochemistry in the PrL region of prefrontal cortex from vehicle group. (f) Representative section of ErbB4 immunohistochemistry in the PrL region of prefrontal cortex from MK-801-treated group. (g) Representative section of ErbB4 immunohistochemistry in the Cg1 and M2 region of prefrontal cortex from vehicle group. (h) Representative section of ErbB4 immunohistochemistry in the Cg1 and M2 region of prefrontal cortex from MK-801-treated group. Arrows indicate ErbB4-expressing neurons in the prefrontal cortex. Scale bar for (e)–(h): 50 μm.

Mentions: Protein samples extracted from rat prefrontal cortex were analyzed by Western blot. Quantitative data showed that expressions of all five NRG1β isoforms were significantly elevated with relative levels (% of vehicle treated controls) of 220 ± 5 for 35 kDa isoform, 245 ± 22 for 65 kDa isoform, 255 ± 23 for 80 kDa isoform, 266 ± 10 for 85 kDa isoform, and 224 ± 18 for 95 kDa isoform, respectively, while all three isoforms of ErbB4 were also significantly upregulated with relative levels (% of vehicle treated controls) of 190 ± 26 for 60 kDa isoform, 164 ± 5 for 120 kDa isoform, and 199 ± 12 for 185 kDa isoform, respectively (Figures 1(a)–1(d)).


Expressions of neuregulin 1beta and ErbB4 in prefrontal cortex and hippocampus of a rat schizophrenia model induced by chronic MK-801 administration.

Feng Y, Wang XD, Guo CM, Yang Y, Li JT, Su YA, Si TM - J. Biomed. Biotechnol. (2010)

Expression of NRG1β and ErbB4 proteins in the rat prefrontal cortex. (a) Representative Western blots of NRG1β protein isoforms. (b) Quantitative analysis of NRG1β immunoblots. (c) Representative Western blots of ErbB4 proteins isoforms. (d) Quantitative analysis of ErbB4 immunoblots. The data were normalized by taking the value of vehicle group as 100% and expressed as means ± S.E.M. (n = 5). *P < .05. (e) Representative section of ErbB4 immunohistochemistry in the PrL region of prefrontal cortex from vehicle group. (f) Representative section of ErbB4 immunohistochemistry in the PrL region of prefrontal cortex from MK-801-treated group. (g) Representative section of ErbB4 immunohistochemistry in the Cg1 and M2 region of prefrontal cortex from vehicle group. (h) Representative section of ErbB4 immunohistochemistry in the Cg1 and M2 region of prefrontal cortex from MK-801-treated group. Arrows indicate ErbB4-expressing neurons in the prefrontal cortex. Scale bar for (e)–(h): 50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2864910&req=5

fig1: Expression of NRG1β and ErbB4 proteins in the rat prefrontal cortex. (a) Representative Western blots of NRG1β protein isoforms. (b) Quantitative analysis of NRG1β immunoblots. (c) Representative Western blots of ErbB4 proteins isoforms. (d) Quantitative analysis of ErbB4 immunoblots. The data were normalized by taking the value of vehicle group as 100% and expressed as means ± S.E.M. (n = 5). *P < .05. (e) Representative section of ErbB4 immunohistochemistry in the PrL region of prefrontal cortex from vehicle group. (f) Representative section of ErbB4 immunohistochemistry in the PrL region of prefrontal cortex from MK-801-treated group. (g) Representative section of ErbB4 immunohistochemistry in the Cg1 and M2 region of prefrontal cortex from vehicle group. (h) Representative section of ErbB4 immunohistochemistry in the Cg1 and M2 region of prefrontal cortex from MK-801-treated group. Arrows indicate ErbB4-expressing neurons in the prefrontal cortex. Scale bar for (e)–(h): 50 μm.
Mentions: Protein samples extracted from rat prefrontal cortex were analyzed by Western blot. Quantitative data showed that expressions of all five NRG1β isoforms were significantly elevated with relative levels (% of vehicle treated controls) of 220 ± 5 for 35 kDa isoform, 245 ± 22 for 65 kDa isoform, 255 ± 23 for 80 kDa isoform, 266 ± 10 for 85 kDa isoform, and 224 ± 18 for 95 kDa isoform, respectively, while all three isoforms of ErbB4 were also significantly upregulated with relative levels (% of vehicle treated controls) of 190 ± 26 for 60 kDa isoform, 164 ± 5 for 120 kDa isoform, and 199 ± 12 for 185 kDa isoform, respectively (Figures 1(a)–1(d)).

Bottom Line: Our data showed that NRG1beta and ErbB4 expressions were significantly increased in the rat prefrontal cortex and hippocampus but in different subregions.These findings suggest that altered expressions of NRG1 and ErbB4 might be attributed to the schizophrenia.Further study in the role and mechanism of NRG1 and ErbB4 may lead to better understanding of the pathophysiology for this disorder.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Mental Health of National Health Ministry, Institute of Mental Health, Peking University, No. 51 Hua Yuan Bei Road, Haidian District, Beijing 100191, China.

ABSTRACT
Recent human genetic studies and postmortem brain examinations of schizophrenia patients strongly indicate that dysregulation of NRG1 and ErbB4 may be important pathogenic factors of schizophrenia. However, this hypothesis has not been validated and fully investigated in animal models of schizophrenia. In this study we quantitatively examined NRG1 and ErbB4 protein expressions by immunohistochemistry and Western blot in the brain of a rat schizophrenia model induced by chronic administration of MK-801 (a noncompetitive NMDA receptor antagonist). Our data showed that NRG1beta and ErbB4 expressions were significantly increased in the rat prefrontal cortex and hippocampus but in different subregions. These findings suggest that altered expressions of NRG1 and ErbB4 might be attributed to the schizophrenia. Further study in the role and mechanism of NRG1 and ErbB4 may lead to better understanding of the pathophysiology for this disorder.

Show MeSH
Related in: MedlinePlus