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Inflammatory molecules in aqueous humour and on ocular surface and glaucoma surgery outcome.

Cvenkel B, Kopitar AN, Ihan A - Mediators Inflamm. (2010)

Bottom Line: Thirty patients who needed antiglaucomatous surgery were included.Eyes with trabeculectomy failure at 3 months showed significantly higher TNF-alpha and IL-6 levels in the aqueous than eyes with successful surgery.Increased expression of HLA-DR on epithelial cells and antigen-presenting cells was not associated with the trabeculectomy outcome.

View Article: PubMed Central - PubMed

Affiliation: Eye Hospital, University Medical Centre Ljubljana, 1000 Ljubljana, Slovenia.

ABSTRACT

Purpose: To investigate the influence of inflammatory molecules in the aqueous humour and on the ocular surface on the outcome of glaucoma surgery.

Methods: Thirty patients who needed antiglaucomatous surgery were included. The interleukin- (IL-) 8, IL-1beta, IL-6, IL-10, tumour necrosis factor- (TNF-) alpha; and IL-12 were determined from aqueous humour preoperatively and the imprints of conjunctiva were analysed for expression of human leukocyte antigen- (HLA-)-DR after surgery by flow cytometry. The success of trabeculectomy was defined as intraocular pressure less than 21 mmHg without antiglaucoma medication.

Results: Eyes with trabeculectomy failure at 3 months showed significantly higher TNF-alpha and IL-6 levels in the aqueous than eyes with successful surgery. Increased expression of HLA-DR on epithelial cells and antigen-presenting cells was not associated with the trabeculectomy outcome.

Conclusions: Higher preoperative levels of TNF-alpha and IL-6 in aqueous humour may contribute to the development of inflammatory milieu and were associated with worse outcome of glaucoma surgery.

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Related in: MedlinePlus

Box plot of expression of HLA-DR on conjunctival epithelial cells (MFI-mean fluorescence intensity) collected by impression cytology 1 month after surgery in eyes with surgical success and failure at 6 months (P = .036). The boundaries of each box indicate the 25th and 75th percentiles, the whiskers indicate the minimum and maximum values, and the line within each box indicates the median.
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fig1: Box plot of expression of HLA-DR on conjunctival epithelial cells (MFI-mean fluorescence intensity) collected by impression cytology 1 month after surgery in eyes with surgical success and failure at 6 months (P = .036). The boundaries of each box indicate the 25th and 75th percentiles, the whiskers indicate the minimum and maximum values, and the line within each box indicates the median.

Mentions: At 3 months of follow-up 23 eyes (patients) had successful surgery (defined as IOP less than 21 mm Hg without antiglaucoma medication), and at 6 and 12 months there were 21 eyes with successful surgery. The median values and ranges for the percentages of HLA-DR positive cytokeratin cells (epithelial cells) and CD80 positive cells (antigen-presenting cells), with their median fluorescence intensity are shown in Table 2. Mean fluorescence intensity of HLA-DR molecules on epithelial cells at 1 month and 3 months was increased in eyes with successful surgery at 12 months, but there was large scatter of the data (Figures 1 and 2).


Inflammatory molecules in aqueous humour and on ocular surface and glaucoma surgery outcome.

Cvenkel B, Kopitar AN, Ihan A - Mediators Inflamm. (2010)

Box plot of expression of HLA-DR on conjunctival epithelial cells (MFI-mean fluorescence intensity) collected by impression cytology 1 month after surgery in eyes with surgical success and failure at 6 months (P = .036). The boundaries of each box indicate the 25th and 75th percentiles, the whiskers indicate the minimum and maximum values, and the line within each box indicates the median.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2864908&req=5

fig1: Box plot of expression of HLA-DR on conjunctival epithelial cells (MFI-mean fluorescence intensity) collected by impression cytology 1 month after surgery in eyes with surgical success and failure at 6 months (P = .036). The boundaries of each box indicate the 25th and 75th percentiles, the whiskers indicate the minimum and maximum values, and the line within each box indicates the median.
Mentions: At 3 months of follow-up 23 eyes (patients) had successful surgery (defined as IOP less than 21 mm Hg without antiglaucoma medication), and at 6 and 12 months there were 21 eyes with successful surgery. The median values and ranges for the percentages of HLA-DR positive cytokeratin cells (epithelial cells) and CD80 positive cells (antigen-presenting cells), with their median fluorescence intensity are shown in Table 2. Mean fluorescence intensity of HLA-DR molecules on epithelial cells at 1 month and 3 months was increased in eyes with successful surgery at 12 months, but there was large scatter of the data (Figures 1 and 2).

Bottom Line: Thirty patients who needed antiglaucomatous surgery were included.Eyes with trabeculectomy failure at 3 months showed significantly higher TNF-alpha and IL-6 levels in the aqueous than eyes with successful surgery.Increased expression of HLA-DR on epithelial cells and antigen-presenting cells was not associated with the trabeculectomy outcome.

View Article: PubMed Central - PubMed

Affiliation: Eye Hospital, University Medical Centre Ljubljana, 1000 Ljubljana, Slovenia.

ABSTRACT

Purpose: To investigate the influence of inflammatory molecules in the aqueous humour and on the ocular surface on the outcome of glaucoma surgery.

Methods: Thirty patients who needed antiglaucomatous surgery were included. The interleukin- (IL-) 8, IL-1beta, IL-6, IL-10, tumour necrosis factor- (TNF-) alpha; and IL-12 were determined from aqueous humour preoperatively and the imprints of conjunctiva were analysed for expression of human leukocyte antigen- (HLA-)-DR after surgery by flow cytometry. The success of trabeculectomy was defined as intraocular pressure less than 21 mmHg without antiglaucoma medication.

Results: Eyes with trabeculectomy failure at 3 months showed significantly higher TNF-alpha and IL-6 levels in the aqueous than eyes with successful surgery. Increased expression of HLA-DR on epithelial cells and antigen-presenting cells was not associated with the trabeculectomy outcome.

Conclusions: Higher preoperative levels of TNF-alpha and IL-6 in aqueous humour may contribute to the development of inflammatory milieu and were associated with worse outcome of glaucoma surgery.

Show MeSH
Related in: MedlinePlus