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68Ga-chloride PET reveals human pancreatic adenocarcinoma xenografts in rats--comparison with FDG.

Ujula T, Salomäki S, Autio A, Luoto P, Tolvanen T, Lehikoinen P, Viljanen T, Sipilä H, Härkönen P, Roivainen A - Mol Imaging Biol (2009)

Bottom Line: The aim of the study was to compare (68)Ga-chloride with 2-[(18)F]fluoro-2-deoxy-D: -glucose (FDG) for the imaging of pancreatic xenografts.Ex vivo studies showed tumor-to-muscle ratio of 4.0 +/- 0.3 for (68)Ga-chloride (n = 4) and 7.9 +/- 3.2 for FDG (n = 4). (68)Ga-chloride delineated subcutaneously implanted pancreatic adenocarcinoma xenografts by PET, but the uptake was lower than FDG.Further studies to clarify the value of (68)Ga-chloride for PET imaging of tumors are warranted.

View Article: PubMed Central - PubMed

Affiliation: Turku PET Centre, Turku University Hospital, 20521, Turku, Finland.

ABSTRACT

Purpose: The aim of the study was to compare (68)Ga-chloride with 2-[(18)F]fluoro-2-deoxy-D: -glucose (FDG) for the imaging of pancreatic xenografts.

Procedures: Rats with subcutaneous human pancreatic adenocarcinoma xenografts were evaluated in vivo by dynamic positron emission tomography (PET) and ex vivo by measuring radioactivity of excised tissues and by digital autoradiography of tumor cryosections.

Results: Both tracers were capable of delineating all subcutaneous tumors from surrounding tissues by PET. The standardized uptake values of tumors by PET were 0.9 +/- 0.3 (mean +/- SD) for (68)Ga-chloride (n = 13) and 1.8 +/- 1.2 for FDG (n = 11). Ex vivo studies showed tumor-to-muscle ratio of 4.0 +/- 0.3 for (68)Ga-chloride (n = 4) and 7.9 +/- 3.2 for FDG (n = 4).

Conclusions: (68)Ga-chloride delineated subcutaneously implanted pancreatic adenocarcinoma xenografts by PET, but the uptake was lower than FDG. Further studies to clarify the value of (68)Ga-chloride for PET imaging of tumors are warranted.

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Representative digital autographs and immunohistological stainings of BxPC-3 tumor cryosections. a68Ga-chloride autoradiography, b macrophage staining with CD68 antibody, and c magnification (×120) of the same section show rather uniform distribution of the radioactivity and macrophages, respectively. The CD68 positive staining is strong also in the stroma.
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Fig7: Representative digital autographs and immunohistological stainings of BxPC-3 tumor cryosections. a68Ga-chloride autoradiography, b macrophage staining with CD68 antibody, and c magnification (×120) of the same section show rather uniform distribution of the radioactivity and macrophages, respectively. The CD68 positive staining is strong also in the stroma.

Mentions: The histological sections of BxPC-3 tumors showed mainly homogenous, viable tumor tissue without any major necrotic areas (Fig. 6). 68Ga-chloride uptake by the tumor was mostly by viable cancer cell islets. Immunohistochemical staining with mouse antirat CD68 recognizing rat macrophages revealed homogenous distribution in the tumor tissue (Fig. 7).Fig. 6


68Ga-chloride PET reveals human pancreatic adenocarcinoma xenografts in rats--comparison with FDG.

Ujula T, Salomäki S, Autio A, Luoto P, Tolvanen T, Lehikoinen P, Viljanen T, Sipilä H, Härkönen P, Roivainen A - Mol Imaging Biol (2009)

Representative digital autographs and immunohistological stainings of BxPC-3 tumor cryosections. a68Ga-chloride autoradiography, b macrophage staining with CD68 antibody, and c magnification (×120) of the same section show rather uniform distribution of the radioactivity and macrophages, respectively. The CD68 positive staining is strong also in the stroma.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2864902&req=5

Fig7: Representative digital autographs and immunohistological stainings of BxPC-3 tumor cryosections. a68Ga-chloride autoradiography, b macrophage staining with CD68 antibody, and c magnification (×120) of the same section show rather uniform distribution of the radioactivity and macrophages, respectively. The CD68 positive staining is strong also in the stroma.
Mentions: The histological sections of BxPC-3 tumors showed mainly homogenous, viable tumor tissue without any major necrotic areas (Fig. 6). 68Ga-chloride uptake by the tumor was mostly by viable cancer cell islets. Immunohistochemical staining with mouse antirat CD68 recognizing rat macrophages revealed homogenous distribution in the tumor tissue (Fig. 7).Fig. 6

Bottom Line: The aim of the study was to compare (68)Ga-chloride with 2-[(18)F]fluoro-2-deoxy-D: -glucose (FDG) for the imaging of pancreatic xenografts.Ex vivo studies showed tumor-to-muscle ratio of 4.0 +/- 0.3 for (68)Ga-chloride (n = 4) and 7.9 +/- 3.2 for FDG (n = 4). (68)Ga-chloride delineated subcutaneously implanted pancreatic adenocarcinoma xenografts by PET, but the uptake was lower than FDG.Further studies to clarify the value of (68)Ga-chloride for PET imaging of tumors are warranted.

View Article: PubMed Central - PubMed

Affiliation: Turku PET Centre, Turku University Hospital, 20521, Turku, Finland.

ABSTRACT

Purpose: The aim of the study was to compare (68)Ga-chloride with 2-[(18)F]fluoro-2-deoxy-D: -glucose (FDG) for the imaging of pancreatic xenografts.

Procedures: Rats with subcutaneous human pancreatic adenocarcinoma xenografts were evaluated in vivo by dynamic positron emission tomography (PET) and ex vivo by measuring radioactivity of excised tissues and by digital autoradiography of tumor cryosections.

Results: Both tracers were capable of delineating all subcutaneous tumors from surrounding tissues by PET. The standardized uptake values of tumors by PET were 0.9 +/- 0.3 (mean +/- SD) for (68)Ga-chloride (n = 13) and 1.8 +/- 1.2 for FDG (n = 11). Ex vivo studies showed tumor-to-muscle ratio of 4.0 +/- 0.3 for (68)Ga-chloride (n = 4) and 7.9 +/- 3.2 for FDG (n = 4).

Conclusions: (68)Ga-chloride delineated subcutaneously implanted pancreatic adenocarcinoma xenografts by PET, but the uptake was lower than FDG. Further studies to clarify the value of (68)Ga-chloride for PET imaging of tumors are warranted.

Show MeSH
Related in: MedlinePlus