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68Ga-chloride PET reveals human pancreatic adenocarcinoma xenografts in rats--comparison with FDG.

Ujula T, Salomäki S, Autio A, Luoto P, Tolvanen T, Lehikoinen P, Viljanen T, Sipilä H, Härkönen P, Roivainen A - Mol Imaging Biol (2009)

Bottom Line: The aim of the study was to compare (68)Ga-chloride with 2-[(18)F]fluoro-2-deoxy-D: -glucose (FDG) for the imaging of pancreatic xenografts.Ex vivo studies showed tumor-to-muscle ratio of 4.0 +/- 0.3 for (68)Ga-chloride (n = 4) and 7.9 +/- 3.2 for FDG (n = 4). (68)Ga-chloride delineated subcutaneously implanted pancreatic adenocarcinoma xenografts by PET, but the uptake was lower than FDG.Further studies to clarify the value of (68)Ga-chloride for PET imaging of tumors are warranted.

View Article: PubMed Central - PubMed

Affiliation: Turku PET Centre, Turku University Hospital, 20521, Turku, Finland.

ABSTRACT

Purpose: The aim of the study was to compare (68)Ga-chloride with 2-[(18)F]fluoro-2-deoxy-D: -glucose (FDG) for the imaging of pancreatic xenografts.

Procedures: Rats with subcutaneous human pancreatic adenocarcinoma xenografts were evaluated in vivo by dynamic positron emission tomography (PET) and ex vivo by measuring radioactivity of excised tissues and by digital autoradiography of tumor cryosections.

Results: Both tracers were capable of delineating all subcutaneous tumors from surrounding tissues by PET. The standardized uptake values of tumors by PET were 0.9 +/- 0.3 (mean +/- SD) for (68)Ga-chloride (n = 13) and 1.8 +/- 1.2 for FDG (n = 11). Ex vivo studies showed tumor-to-muscle ratio of 4.0 +/- 0.3 for (68)Ga-chloride (n = 4) and 7.9 +/- 3.2 for FDG (n = 4).

Conclusions: (68)Ga-chloride delineated subcutaneously implanted pancreatic adenocarcinoma xenografts by PET, but the uptake was lower than FDG. Further studies to clarify the value of (68)Ga-chloride for PET imaging of tumors are warranted.

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Representative digital autoradiographs of BxPC-3 tumor cryosections and corresponding hematoxylin and eosin stainings. a68Ga-chloride autoradiography at 90 min postinjection (p.i.) and b the HE-staining of the same section. c FDG autoradiography at 90 min p.i. and d the corresponding HE staining. The dark blue represents the lowest and hot red the highest amount of radioactivity.
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Fig5: Representative digital autoradiographs of BxPC-3 tumor cryosections and corresponding hematoxylin and eosin stainings. a68Ga-chloride autoradiography at 90 min postinjection (p.i.) and b the HE-staining of the same section. c FDG autoradiography at 90 min p.i. and d the corresponding HE staining. The dark blue represents the lowest and hot red the highest amount of radioactivity.

Mentions: Representative digital autoradiographs of 68Ga-chloride and FDG distribution in the tumor tissue are shown in Fig. 5. The imaging agents showed different biodistribution within the tumor. The 68Ga radioactivity was quite homogeneously distributed, while distribution of FDG was more heterogenous.Fig. 5


68Ga-chloride PET reveals human pancreatic adenocarcinoma xenografts in rats--comparison with FDG.

Ujula T, Salomäki S, Autio A, Luoto P, Tolvanen T, Lehikoinen P, Viljanen T, Sipilä H, Härkönen P, Roivainen A - Mol Imaging Biol (2009)

Representative digital autoradiographs of BxPC-3 tumor cryosections and corresponding hematoxylin and eosin stainings. a68Ga-chloride autoradiography at 90 min postinjection (p.i.) and b the HE-staining of the same section. c FDG autoradiography at 90 min p.i. and d the corresponding HE staining. The dark blue represents the lowest and hot red the highest amount of radioactivity.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2864902&req=5

Fig5: Representative digital autoradiographs of BxPC-3 tumor cryosections and corresponding hematoxylin and eosin stainings. a68Ga-chloride autoradiography at 90 min postinjection (p.i.) and b the HE-staining of the same section. c FDG autoradiography at 90 min p.i. and d the corresponding HE staining. The dark blue represents the lowest and hot red the highest amount of radioactivity.
Mentions: Representative digital autoradiographs of 68Ga-chloride and FDG distribution in the tumor tissue are shown in Fig. 5. The imaging agents showed different biodistribution within the tumor. The 68Ga radioactivity was quite homogeneously distributed, while distribution of FDG was more heterogenous.Fig. 5

Bottom Line: The aim of the study was to compare (68)Ga-chloride with 2-[(18)F]fluoro-2-deoxy-D: -glucose (FDG) for the imaging of pancreatic xenografts.Ex vivo studies showed tumor-to-muscle ratio of 4.0 +/- 0.3 for (68)Ga-chloride (n = 4) and 7.9 +/- 3.2 for FDG (n = 4). (68)Ga-chloride delineated subcutaneously implanted pancreatic adenocarcinoma xenografts by PET, but the uptake was lower than FDG.Further studies to clarify the value of (68)Ga-chloride for PET imaging of tumors are warranted.

View Article: PubMed Central - PubMed

Affiliation: Turku PET Centre, Turku University Hospital, 20521, Turku, Finland.

ABSTRACT

Purpose: The aim of the study was to compare (68)Ga-chloride with 2-[(18)F]fluoro-2-deoxy-D: -glucose (FDG) for the imaging of pancreatic xenografts.

Procedures: Rats with subcutaneous human pancreatic adenocarcinoma xenografts were evaluated in vivo by dynamic positron emission tomography (PET) and ex vivo by measuring radioactivity of excised tissues and by digital autoradiography of tumor cryosections.

Results: Both tracers were capable of delineating all subcutaneous tumors from surrounding tissues by PET. The standardized uptake values of tumors by PET were 0.9 +/- 0.3 (mean +/- SD) for (68)Ga-chloride (n = 13) and 1.8 +/- 1.2 for FDG (n = 11). Ex vivo studies showed tumor-to-muscle ratio of 4.0 +/- 0.3 for (68)Ga-chloride (n = 4) and 7.9 +/- 3.2 for FDG (n = 4).

Conclusions: (68)Ga-chloride delineated subcutaneously implanted pancreatic adenocarcinoma xenografts by PET, but the uptake was lower than FDG. Further studies to clarify the value of (68)Ga-chloride for PET imaging of tumors are warranted.

Show MeSH
Related in: MedlinePlus