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c-Fos expression during temporal order judgment in mice.

Wada M, Higo N, Moizumi S, Kitazawa S - PLoS ONE (2010)

Bottom Line: The expression of c-Fos was significantly higher in the test group than in the other groups in the bilateral barrel fields of the primary somatosensory cortex, the left secondary somatosensory cortex, the dorsal part of the right secondary auditory cortex.Laminar analyses in the primary somatosensory cortex revealed that c-Fos expression in the test group was most evident in layers II and III, where callosal fibers project.The results suggest that temporal order judgment involves processing bilateral somatosensory signals through the supragranular layers of the primary sensory cortex and in the multimodal sensory areas, including marginal zone between the primary somatosensory cortex and the secondary sensory cortex.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Juntendo University School of Medicine, Tokyo, Japan. m-wada@juntendo.ac.jp

ABSTRACT
The neuronal mechanisms for ordering sensory signals in time still need to be clarified despite a long history of research. To address this issue, we recently developed a behavioral task of temporal order judgment in mice. In the present study, we examined the expression of c-Fos, a marker of neural activation, in mice just after they carried out the temporal order judgment task. The expression of c-Fos was examined in C57BL/6N mice (male, n = 5) that were trained to judge the order of two air-puff stimuli delivered bilaterally to the right and left whiskers with stimulation intervals of 50-750 ms. The mice were rewarded with a food pellet when they responded by orienting their head toward the first stimulus (n = 2) or toward the second stimulus (n = 3) after a visual "go" signal. c-Fos-stained cell densities of these mice (test group) were compared with those of two control groups in coronal brain sections prepared at bregma -2, -1, 0, +1, and +2 mm by applying statistical parametric mapping to the c-Fos immuno-stained sections. The expression of c-Fos was significantly higher in the test group than in the other groups in the bilateral barrel fields of the primary somatosensory cortex, the left secondary somatosensory cortex, the dorsal part of the right secondary auditory cortex. Laminar analyses in the primary somatosensory cortex revealed that c-Fos expression in the test group was most evident in layers II and III, where callosal fibers project. The results suggest that temporal order judgment involves processing bilateral somatosensory signals through the supragranular layers of the primary sensory cortex and in the multimodal sensory areas, including marginal zone between the primary somatosensory cortex and the secondary sensory cortex.

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Double-staining with c-Fos and other antibodies.Images were taken from the barrel field of the mice in the test group. (A) c-Fos (green) and GFAP (red). No co-localization was observed. (B) c-Fos (green) and NeuN (red). Most cells were double-stained (yellow). (C) c-Fos (green) and CAMKIIα (red). Some cells were double-stained (arrow heads). (D) c-Fos (red) and GABA (green). Some cells were double-stained (arrows).
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pone-0010483-g006: Double-staining with c-Fos and other antibodies.Images were taken from the barrel field of the mice in the test group. (A) c-Fos (green) and GFAP (red). No co-localization was observed. (B) c-Fos (green) and NeuN (red). Most cells were double-stained (yellow). (C) c-Fos (green) and CAMKIIα (red). Some cells were double-stained (arrow heads). (D) c-Fos (red) and GABA (green). Some cells were double-stained (arrows).

Mentions: We further investigated the cellular types of the c-Fos-immunoreactive cells using double-labeling methods. We found that c-Fos never co-localized with GFAP, an astroglial marker (Fig. 6A), but generally (98%) co-localized with NeuN, a neuronal marker (Fig. 6B). We found that 26±4% (mean±s.e.m, 31 sections) of c-Fos positive cells were double-stained with CAMKIIα (e.g. Fig. 6A), a marker of excitatory neurons [37], while 21±4% (mean±s.e.m, 42 sections) were labeled with GABA (Fig. 6D). These results indicate that c-Fos positive cells in the test group were not glial cells but neuronal cells and that they were mixtures of excitatory neurons and inhibitory neurons.


c-Fos expression during temporal order judgment in mice.

Wada M, Higo N, Moizumi S, Kitazawa S - PLoS ONE (2010)

Double-staining with c-Fos and other antibodies.Images were taken from the barrel field of the mice in the test group. (A) c-Fos (green) and GFAP (red). No co-localization was observed. (B) c-Fos (green) and NeuN (red). Most cells were double-stained (yellow). (C) c-Fos (green) and CAMKIIα (red). Some cells were double-stained (arrow heads). (D) c-Fos (red) and GABA (green). Some cells were double-stained (arrows).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2864740&req=5

pone-0010483-g006: Double-staining with c-Fos and other antibodies.Images were taken from the barrel field of the mice in the test group. (A) c-Fos (green) and GFAP (red). No co-localization was observed. (B) c-Fos (green) and NeuN (red). Most cells were double-stained (yellow). (C) c-Fos (green) and CAMKIIα (red). Some cells were double-stained (arrow heads). (D) c-Fos (red) and GABA (green). Some cells were double-stained (arrows).
Mentions: We further investigated the cellular types of the c-Fos-immunoreactive cells using double-labeling methods. We found that c-Fos never co-localized with GFAP, an astroglial marker (Fig. 6A), but generally (98%) co-localized with NeuN, a neuronal marker (Fig. 6B). We found that 26±4% (mean±s.e.m, 31 sections) of c-Fos positive cells were double-stained with CAMKIIα (e.g. Fig. 6A), a marker of excitatory neurons [37], while 21±4% (mean±s.e.m, 42 sections) were labeled with GABA (Fig. 6D). These results indicate that c-Fos positive cells in the test group were not glial cells but neuronal cells and that they were mixtures of excitatory neurons and inhibitory neurons.

Bottom Line: The expression of c-Fos was significantly higher in the test group than in the other groups in the bilateral barrel fields of the primary somatosensory cortex, the left secondary somatosensory cortex, the dorsal part of the right secondary auditory cortex.Laminar analyses in the primary somatosensory cortex revealed that c-Fos expression in the test group was most evident in layers II and III, where callosal fibers project.The results suggest that temporal order judgment involves processing bilateral somatosensory signals through the supragranular layers of the primary sensory cortex and in the multimodal sensory areas, including marginal zone between the primary somatosensory cortex and the secondary sensory cortex.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Juntendo University School of Medicine, Tokyo, Japan. m-wada@juntendo.ac.jp

ABSTRACT
The neuronal mechanisms for ordering sensory signals in time still need to be clarified despite a long history of research. To address this issue, we recently developed a behavioral task of temporal order judgment in mice. In the present study, we examined the expression of c-Fos, a marker of neural activation, in mice just after they carried out the temporal order judgment task. The expression of c-Fos was examined in C57BL/6N mice (male, n = 5) that were trained to judge the order of two air-puff stimuli delivered bilaterally to the right and left whiskers with stimulation intervals of 50-750 ms. The mice were rewarded with a food pellet when they responded by orienting their head toward the first stimulus (n = 2) or toward the second stimulus (n = 3) after a visual "go" signal. c-Fos-stained cell densities of these mice (test group) were compared with those of two control groups in coronal brain sections prepared at bregma -2, -1, 0, +1, and +2 mm by applying statistical parametric mapping to the c-Fos immuno-stained sections. The expression of c-Fos was significantly higher in the test group than in the other groups in the bilateral barrel fields of the primary somatosensory cortex, the left secondary somatosensory cortex, the dorsal part of the right secondary auditory cortex. Laminar analyses in the primary somatosensory cortex revealed that c-Fos expression in the test group was most evident in layers II and III, where callosal fibers project. The results suggest that temporal order judgment involves processing bilateral somatosensory signals through the supragranular layers of the primary sensory cortex and in the multimodal sensory areas, including marginal zone between the primary somatosensory cortex and the secondary sensory cortex.

Show MeSH