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Photoacoustic ophthalmoscopy for in vivo retinal imaging.

Jiao S, Jiang M, Hu J, Fawzi A, Zhou Q, Shung KK, Puliafito CA, Zhang HF - Opt Express (2010)

Bottom Line: For B-scan frames containing 256 A-lines, the current PAOM has a frame rate of 93 Hz, which is comparable with state-of-the-art commercial spectral-domain optical coherence tomography (SD-OCT).By integrating PAOM with SD-OCT, we further achieved OCT-guided PAOM, which can provide multi-modal retinal imaging simultaneously.The capabilities of this novel technology were demonstrated by imaging both the microanatomy and microvasculature of the rat retina in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.

ABSTRACT
We have developed a non-invasive photoacoustic ophthalmoscopy (PAOM) for in vivo retinal imaging. PAOM detects the photoacoustic signal induced by pulsed laser light shined onto the retina. By using a stationary ultrasonic transducer in contact with the eyelids and scanning only the laser light across the retina, PAOM provides volumetric imaging of the retinal micro-vasculature and retinal pigment epithelium at a high speed. For B-scan frames containing 256 A-lines, the current PAOM has a frame rate of 93 Hz, which is comparable with state-of-the-art commercial spectral-domain optical coherence tomography (SD-OCT). By integrating PAOM with SD-OCT, we further achieved OCT-guided PAOM, which can provide multi-modal retinal imaging simultaneously. The capabilities of this novel technology were demonstrated by imaging both the microanatomy and microvasculature of the rat retina in vivo.

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Related in: MedlinePlus

Comparison of OCT and PAOM images acquired simultaneously in vivo. (a) PAOM B-scan image in pseudocolors; (b) OCT B-scan image; (c) MAP image of the PAOM data set. Bar: 100 µm.
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g002: Comparison of OCT and PAOM images acquired simultaneously in vivo. (a) PAOM B-scan image in pseudocolors; (b) OCT B-scan image; (c) MAP image of the PAOM data set. Bar: 100 µm.

Mentions: Figure 2Fig. 2


Photoacoustic ophthalmoscopy for in vivo retinal imaging.

Jiao S, Jiang M, Hu J, Fawzi A, Zhou Q, Shung KK, Puliafito CA, Zhang HF - Opt Express (2010)

Comparison of OCT and PAOM images acquired simultaneously in vivo. (a) PAOM B-scan image in pseudocolors; (b) OCT B-scan image; (c) MAP image of the PAOM data set. Bar: 100 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2864517&req=5

g002: Comparison of OCT and PAOM images acquired simultaneously in vivo. (a) PAOM B-scan image in pseudocolors; (b) OCT B-scan image; (c) MAP image of the PAOM data set. Bar: 100 µm.
Mentions: Figure 2Fig. 2

Bottom Line: For B-scan frames containing 256 A-lines, the current PAOM has a frame rate of 93 Hz, which is comparable with state-of-the-art commercial spectral-domain optical coherence tomography (SD-OCT).By integrating PAOM with SD-OCT, we further achieved OCT-guided PAOM, which can provide multi-modal retinal imaging simultaneously.The capabilities of this novel technology were demonstrated by imaging both the microanatomy and microvasculature of the rat retina in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.

ABSTRACT
We have developed a non-invasive photoacoustic ophthalmoscopy (PAOM) for in vivo retinal imaging. PAOM detects the photoacoustic signal induced by pulsed laser light shined onto the retina. By using a stationary ultrasonic transducer in contact with the eyelids and scanning only the laser light across the retina, PAOM provides volumetric imaging of the retinal micro-vasculature and retinal pigment epithelium at a high speed. For B-scan frames containing 256 A-lines, the current PAOM has a frame rate of 93 Hz, which is comparable with state-of-the-art commercial spectral-domain optical coherence tomography (SD-OCT). By integrating PAOM with SD-OCT, we further achieved OCT-guided PAOM, which can provide multi-modal retinal imaging simultaneously. The capabilities of this novel technology were demonstrated by imaging both the microanatomy and microvasculature of the rat retina in vivo.

Show MeSH
Related in: MedlinePlus