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The behaviour of both Listeria monocytogenes and rat ciliated ependymal cells is altered during their co-culture.

Fadaee-Shohada MJ, Hirst RA, Rutman A, Roberts IS, O'Callaghan C, Andrew PW - PLoS ONE (2010)

Bottom Line: Ciliated ependymal cells line the cerebral ventricles and aqueducts separating the infected CSF from the brain parenchyma in meningitis.Investigation of the interaction of Listeria monocytogenes with cultured rat brain ependymal cells showed that certain strains reduced the beat frequency of the cilia but all the strains studied significantly reduced the ciliary beat amplitude (the linear distance travelled by the tip of each cilium per beat cycle).The presence of the ependyma caused aggregation of some listeria strains and in some cases extracellular material also was seen in association with bacterial aggregates.

View Article: PubMed Central - PubMed

Affiliation: Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, United Kingdom.

ABSTRACT

Background: Ciliated ependymal cells line the cerebral ventricles and aqueducts separating the infected CSF from the brain parenchyma in meningitis.

Principal findings: Investigation of the interaction of Listeria monocytogenes with cultured rat brain ependymal cells showed that certain strains reduced the beat frequency of the cilia but all the strains studied significantly reduced the ciliary beat amplitude (the linear distance travelled by the tip of each cilium per beat cycle).

Conclusion: The presence of the ependyma caused aggregation of some listeria strains and in some cases extracellular material also was seen in association with bacterial aggregates. These observations were dependent on the expression of genes required for invasion, intracellular survival and listerial cell to cell spread that are regulated by the transcriptional activator, positive regulatory factor A (PrfA).

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Related in: MedlinePlus

Scanning electron microscope image of listeria and extracellular material.L. monocytogenes strain 10403S was embedded within the extracellular material. The arrow is pointing to a strand of extracellular material between the bacteria. Scale bar represents 1 µm.
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pone-0010450-g002: Scanning electron microscope image of listeria and extracellular material.L. monocytogenes strain 10403S was embedded within the extracellular material. The arrow is pointing to a strand of extracellular material between the bacteria. Scale bar represents 1 µm.

Mentions: After three hours incubation with EGDe, there was no decrease in ciliary beat frequency compared to the control (Table 2, Video S1). Incubation with the EGDe strain initially resulted in long chains of bacteria attaching to the cilia, moving at the same beat frequency as the cilia (Video S3). After three hours there were large areas of bacterial aggregates in an extracellular material (Video S4). Approximately 61% of the surface area of the ependymal cell culture was covered by listerial aggregates in association with extracellular material (Table 2, Fig. 1E and 1F). The SEM image in Figure 2 shows strands of material between the wild type EGDe strain. Where aggregates were present, there was a 64% reduction in ciliary amplitude (P<0.001, Table 3). Where cilia were not covered by listerial aggregates, the ciliary beat amplitude was also decreased by 19% (P<0.001, Table 3). The reduction in ciliary amplitude appeared to be dependent on the size of the bacterial aggregates. The ciliary amplitude at 3 hours co-culture was markedly reduced compared to the 2 hour amplitude, at which time the bacterial aggregates were smaller (data not shown). From confocal images we occasionally saw bacteria inside the cells, but the majority of cells were not seen to be infected (data not shown). With each strain, bacterial aggregates and extracellular material formed only during contact between the ependymal cells and the bacteria. No aggregates or extracellular material were visible after incubation of the bacteria and medium taken from the ependymal cell cultures.


The behaviour of both Listeria monocytogenes and rat ciliated ependymal cells is altered during their co-culture.

Fadaee-Shohada MJ, Hirst RA, Rutman A, Roberts IS, O'Callaghan C, Andrew PW - PLoS ONE (2010)

Scanning electron microscope image of listeria and extracellular material.L. monocytogenes strain 10403S was embedded within the extracellular material. The arrow is pointing to a strand of extracellular material between the bacteria. Scale bar represents 1 µm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2864257&req=5

pone-0010450-g002: Scanning electron microscope image of listeria and extracellular material.L. monocytogenes strain 10403S was embedded within the extracellular material. The arrow is pointing to a strand of extracellular material between the bacteria. Scale bar represents 1 µm.
Mentions: After three hours incubation with EGDe, there was no decrease in ciliary beat frequency compared to the control (Table 2, Video S1). Incubation with the EGDe strain initially resulted in long chains of bacteria attaching to the cilia, moving at the same beat frequency as the cilia (Video S3). After three hours there were large areas of bacterial aggregates in an extracellular material (Video S4). Approximately 61% of the surface area of the ependymal cell culture was covered by listerial aggregates in association with extracellular material (Table 2, Fig. 1E and 1F). The SEM image in Figure 2 shows strands of material between the wild type EGDe strain. Where aggregates were present, there was a 64% reduction in ciliary amplitude (P<0.001, Table 3). Where cilia were not covered by listerial aggregates, the ciliary beat amplitude was also decreased by 19% (P<0.001, Table 3). The reduction in ciliary amplitude appeared to be dependent on the size of the bacterial aggregates. The ciliary amplitude at 3 hours co-culture was markedly reduced compared to the 2 hour amplitude, at which time the bacterial aggregates were smaller (data not shown). From confocal images we occasionally saw bacteria inside the cells, but the majority of cells were not seen to be infected (data not shown). With each strain, bacterial aggregates and extracellular material formed only during contact between the ependymal cells and the bacteria. No aggregates or extracellular material were visible after incubation of the bacteria and medium taken from the ependymal cell cultures.

Bottom Line: Ciliated ependymal cells line the cerebral ventricles and aqueducts separating the infected CSF from the brain parenchyma in meningitis.Investigation of the interaction of Listeria monocytogenes with cultured rat brain ependymal cells showed that certain strains reduced the beat frequency of the cilia but all the strains studied significantly reduced the ciliary beat amplitude (the linear distance travelled by the tip of each cilium per beat cycle).The presence of the ependyma caused aggregation of some listeria strains and in some cases extracellular material also was seen in association with bacterial aggregates.

View Article: PubMed Central - PubMed

Affiliation: Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, United Kingdom.

ABSTRACT

Background: Ciliated ependymal cells line the cerebral ventricles and aqueducts separating the infected CSF from the brain parenchyma in meningitis.

Principal findings: Investigation of the interaction of Listeria monocytogenes with cultured rat brain ependymal cells showed that certain strains reduced the beat frequency of the cilia but all the strains studied significantly reduced the ciliary beat amplitude (the linear distance travelled by the tip of each cilium per beat cycle).

Conclusion: The presence of the ependyma caused aggregation of some listeria strains and in some cases extracellular material also was seen in association with bacterial aggregates. These observations were dependent on the expression of genes required for invasion, intracellular survival and listerial cell to cell spread that are regulated by the transcriptional activator, positive regulatory factor A (PrfA).

Show MeSH
Related in: MedlinePlus