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The activity of pregnancy-associated plasma protein A (PAPP-A) as expressed by immunohistochemistry in atherothrombotic plaques obtained by aspiration thrombectomy in patients presenting with a ST-elevation myocardial infarction: a brief communication.

Brügger-Andersen T, Bostad L, Sandnes DA, Larsen AI, Bonarjee VV, Barvik S, Melberg T, Nilsen DW - Thromb J (2010)

Bottom Line: The expression of pregnancy-associated plasma protein A (PAPP-A) was identified by immunohistochemistry (IHC) in culprit atherothrombotic plaque specimens harvested from patients admitted with ST-segment elevation myocardial infarction (STEMI).The atherothrombotic samples were collected from a consecutive cohort consisting of 20 individuals admitted with STEMI to Stavanger University Hospital, Norway, from 2005-2006, presenting angiographically with an acute thrombotic occlusion of a coronary artery characterized by TIMI flow 0.The atherothrombotic plaques were obtained by aspiration thrombectomy during percutaneous coronary intervention within 12 hours from the onset of symptoms and prepared for IHC analysis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Medicine, University of Bergen, 5021 Bergen, Norway. hagerman@online.no.

ABSTRACT

Background: The expression of pregnancy-associated plasma protein A (PAPP-A) was identified by immunohistochemistry (IHC) in culprit atherothrombotic plaque specimens harvested from patients admitted with ST-segment elevation myocardial infarction (STEMI).

Methods: The atherothrombotic samples were collected from a consecutive cohort consisting of 20 individuals admitted with STEMI to Stavanger University Hospital, Norway, from 2005-2006, presenting angiographically with an acute thrombotic occlusion of a coronary artery characterized by TIMI flow 0. The atherothrombotic plaques were obtained by aspiration thrombectomy during percutaneous coronary intervention within 12 hours from the onset of symptoms and prepared for IHC analysis.

Results: In the IHC analysis staining for PAPP-A occurred in the extracellular matrix of the plaques and no evidence of staining for PAPP-A was found in the thrombi.

Conclusion: Our results indicate that in vivo PAPP-A is strongly expressed in atherothrombotic plaques harvested from patients admitted with STEMI, as documented by IHC.

Trial registration: biobankregisteret@fhi.no1846.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemistry of placental tissue (positive control). The trophoblastic layer is strongly expressing PAPP-A (reddish brown staining).
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Figure 1: Immunohistochemistry of placental tissue (positive control). The trophoblastic layer is strongly expressing PAPP-A (reddish brown staining).

Mentions: The formalin treated samples were first stored one hour in 70% ethanol, and then embedded in paraffin. Briefly, 4-μm-thick sections from the paraffin blocks were air-dried on superfrost slides, and then frozen until use. Sections from all the blocks were stained with haematoxylin and eosin for general orientation and identification of tissues and structures. The slides for IHC were deparaffinised with toluene, rehydrated in ethanol, boiled in 10 mM Citratebuffer pH 6.0 and TBS-0.025% Tweenbuffer pH 7.6, and incubated with the primary antibody; the polyclonal rabbit anti-human Pregnancy Associated Plasma Protein A: Ig fraction (A0230 Dako), Denmark. For IHC detection and visualisation we used the DakoCytomation EnVision+ System HRP, Rabbit K4010, Dako, Denmark. Furthermore, histological specimens from surgically removed human placenta tissue were used as positive controls (Figure 1).


The activity of pregnancy-associated plasma protein A (PAPP-A) as expressed by immunohistochemistry in atherothrombotic plaques obtained by aspiration thrombectomy in patients presenting with a ST-elevation myocardial infarction: a brief communication.

Brügger-Andersen T, Bostad L, Sandnes DA, Larsen AI, Bonarjee VV, Barvik S, Melberg T, Nilsen DW - Thromb J (2010)

Immunohistochemistry of placental tissue (positive control). The trophoblastic layer is strongly expressing PAPP-A (reddish brown staining).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2837619&req=5

Figure 1: Immunohistochemistry of placental tissue (positive control). The trophoblastic layer is strongly expressing PAPP-A (reddish brown staining).
Mentions: The formalin treated samples were first stored one hour in 70% ethanol, and then embedded in paraffin. Briefly, 4-μm-thick sections from the paraffin blocks were air-dried on superfrost slides, and then frozen until use. Sections from all the blocks were stained with haematoxylin and eosin for general orientation and identification of tissues and structures. The slides for IHC were deparaffinised with toluene, rehydrated in ethanol, boiled in 10 mM Citratebuffer pH 6.0 and TBS-0.025% Tweenbuffer pH 7.6, and incubated with the primary antibody; the polyclonal rabbit anti-human Pregnancy Associated Plasma Protein A: Ig fraction (A0230 Dako), Denmark. For IHC detection and visualisation we used the DakoCytomation EnVision+ System HRP, Rabbit K4010, Dako, Denmark. Furthermore, histological specimens from surgically removed human placenta tissue were used as positive controls (Figure 1).

Bottom Line: The expression of pregnancy-associated plasma protein A (PAPP-A) was identified by immunohistochemistry (IHC) in culprit atherothrombotic plaque specimens harvested from patients admitted with ST-segment elevation myocardial infarction (STEMI).The atherothrombotic samples were collected from a consecutive cohort consisting of 20 individuals admitted with STEMI to Stavanger University Hospital, Norway, from 2005-2006, presenting angiographically with an acute thrombotic occlusion of a coronary artery characterized by TIMI flow 0.The atherothrombotic plaques were obtained by aspiration thrombectomy during percutaneous coronary intervention within 12 hours from the onset of symptoms and prepared for IHC analysis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Medicine, University of Bergen, 5021 Bergen, Norway. hagerman@online.no.

ABSTRACT

Background: The expression of pregnancy-associated plasma protein A (PAPP-A) was identified by immunohistochemistry (IHC) in culprit atherothrombotic plaque specimens harvested from patients admitted with ST-segment elevation myocardial infarction (STEMI).

Methods: The atherothrombotic samples were collected from a consecutive cohort consisting of 20 individuals admitted with STEMI to Stavanger University Hospital, Norway, from 2005-2006, presenting angiographically with an acute thrombotic occlusion of a coronary artery characterized by TIMI flow 0. The atherothrombotic plaques were obtained by aspiration thrombectomy during percutaneous coronary intervention within 12 hours from the onset of symptoms and prepared for IHC analysis.

Results: In the IHC analysis staining for PAPP-A occurred in the extracellular matrix of the plaques and no evidence of staining for PAPP-A was found in the thrombi.

Conclusion: Our results indicate that in vivo PAPP-A is strongly expressed in atherothrombotic plaques harvested from patients admitted with STEMI, as documented by IHC.

Trial registration: biobankregisteret@fhi.no1846.

No MeSH data available.


Related in: MedlinePlus