Limits...
Gene expression profiling demonstrates a novel role for foetal fibrocytes and the umbilical vessels in human fetoplacental development.

Kim JS, Romero R, Tarca AL, LaJeunesse C, Han YM, Kim MJ, Suh YL, Draghici S, Mittal P, Gotsch F, Kusanovic JP, Hassan S, Kim CJ - J. Cell. Mol. Med. (2008)

Bottom Line: A significant proportion of these cells co-expressed CD45 and type I pro-collagen, and acquired CD163 or alpha-smooth muscle actin immunoreactivity in Wharton's jelly.Migrating cells were also found in the chorionic and stem villous vessels.The observations herein strongly suggest that circulating foetal fibrocytes, routing via umbilical and placental vessels, are a reservoir for key cellular subsets in the placenta.

View Article: PubMed Central - PubMed

Affiliation: Perinatology Research Branch, NICHD/NIH/DHHS, Bethesda, MD, USA.

ABSTRACT
There is a difference in the susceptibility to inflammation between the umbilical vein (UV) and the umbilical arteries (UAs). This led us to hypothesize that there is an intrinsic difference in the pro-inflammatory response between UA and UV. Real-time quantitative RT-PCR and microarray analysis revealed higher expression of interleukin (IL)-1beta and IL-8 mRNA in the UV and differential expression of 567 genes between the UA and UV associated with distinct biological processes, including the immune response. Differential expression of human leukocyte antigen (HLA)-DRA mRNA between the UA and UV was due to unexpected HLA-DR(+) cells migrating via the umbilical vessels into Wharton's jelly, more frequently in the UV. A significant proportion of these cells co-expressed CD45 and type I pro-collagen, and acquired CD163 or alpha-smooth muscle actin immunoreactivity in Wharton's jelly. Migrating cells were also found in the chorionic and stem villous vessels. Furthermore, the extent of migration increased with progression of gestation, but diminished in intrauterine growth restriction (IUGR). The observations herein strongly suggest that circulating foetal fibrocytes, routing via umbilical and placental vessels, are a reservoir for key cellular subsets in the placenta. This study reports fibrocytes in the human umbilical cord and placenta for the first time, and a novel role for both circulating foetal cells and the umbilical vessels in placental development, which is deranged in IUGR.

Show MeSH

Related in: MedlinePlus

Differential gene expression involved in the pathway of antigen presentation by MHC class II. A diagram showing the pathway of antigen presentation by MHC class II, which was most significantly associated with the differential gene expression between the UA and UV. The genes expressed higher in UV than those in the UA are indicated by red bars.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2837362&req=5

fig05: Differential gene expression involved in the pathway of antigen presentation by MHC class II. A diagram showing the pathway of antigen presentation by MHC class II, which was most significantly associated with the differential gene expression between the UA and UV. The genes expressed higher in UV than those in the UA are indicated by red bars.

Mentions: GO analysis revealed over-representation of distinct biological processes between the UA and UV (Table 1). The following biological processes were enriched: N-acetylglucosamine metabolism, immune response, response to wounding, organ morphogenesis, apoptosis and smooth muscle contraction. The analysis of the metabolic and signalling pathways contained in the Metacore database yielded five pathways significantly associated with the condition under study (Table 2). These pathways are antigen presentation by major histocompatibility complex (MHC) class II (Fig. 5), reverse signalling by ephrin B, CXCR4 signalling pathway, immunological synapse formation and Notch signalling pathway. The impact analysis using pathway express identified two highly impacted pathways: (i) cell adhesion molecules and (ii) antigen processing and presentation.


Gene expression profiling demonstrates a novel role for foetal fibrocytes and the umbilical vessels in human fetoplacental development.

Kim JS, Romero R, Tarca AL, LaJeunesse C, Han YM, Kim MJ, Suh YL, Draghici S, Mittal P, Gotsch F, Kusanovic JP, Hassan S, Kim CJ - J. Cell. Mol. Med. (2008)

Differential gene expression involved in the pathway of antigen presentation by MHC class II. A diagram showing the pathway of antigen presentation by MHC class II, which was most significantly associated with the differential gene expression between the UA and UV. The genes expressed higher in UV than those in the UA are indicated by red bars.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2837362&req=5

fig05: Differential gene expression involved in the pathway of antigen presentation by MHC class II. A diagram showing the pathway of antigen presentation by MHC class II, which was most significantly associated with the differential gene expression between the UA and UV. The genes expressed higher in UV than those in the UA are indicated by red bars.
Mentions: GO analysis revealed over-representation of distinct biological processes between the UA and UV (Table 1). The following biological processes were enriched: N-acetylglucosamine metabolism, immune response, response to wounding, organ morphogenesis, apoptosis and smooth muscle contraction. The analysis of the metabolic and signalling pathways contained in the Metacore database yielded five pathways significantly associated with the condition under study (Table 2). These pathways are antigen presentation by major histocompatibility complex (MHC) class II (Fig. 5), reverse signalling by ephrin B, CXCR4 signalling pathway, immunological synapse formation and Notch signalling pathway. The impact analysis using pathway express identified two highly impacted pathways: (i) cell adhesion molecules and (ii) antigen processing and presentation.

Bottom Line: A significant proportion of these cells co-expressed CD45 and type I pro-collagen, and acquired CD163 or alpha-smooth muscle actin immunoreactivity in Wharton's jelly.Migrating cells were also found in the chorionic and stem villous vessels.The observations herein strongly suggest that circulating foetal fibrocytes, routing via umbilical and placental vessels, are a reservoir for key cellular subsets in the placenta.

View Article: PubMed Central - PubMed

Affiliation: Perinatology Research Branch, NICHD/NIH/DHHS, Bethesda, MD, USA.

ABSTRACT
There is a difference in the susceptibility to inflammation between the umbilical vein (UV) and the umbilical arteries (UAs). This led us to hypothesize that there is an intrinsic difference in the pro-inflammatory response between UA and UV. Real-time quantitative RT-PCR and microarray analysis revealed higher expression of interleukin (IL)-1beta and IL-8 mRNA in the UV and differential expression of 567 genes between the UA and UV associated with distinct biological processes, including the immune response. Differential expression of human leukocyte antigen (HLA)-DRA mRNA between the UA and UV was due to unexpected HLA-DR(+) cells migrating via the umbilical vessels into Wharton's jelly, more frequently in the UV. A significant proportion of these cells co-expressed CD45 and type I pro-collagen, and acquired CD163 or alpha-smooth muscle actin immunoreactivity in Wharton's jelly. Migrating cells were also found in the chorionic and stem villous vessels. Furthermore, the extent of migration increased with progression of gestation, but diminished in intrauterine growth restriction (IUGR). The observations herein strongly suggest that circulating foetal fibrocytes, routing via umbilical and placental vessels, are a reservoir for key cellular subsets in the placenta. This study reports fibrocytes in the human umbilical cord and placenta for the first time, and a novel role for both circulating foetal cells and the umbilical vessels in placental development, which is deranged in IUGR.

Show MeSH
Related in: MedlinePlus